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Title:          Analysis of the Long-Lived Responses Induced by Immunostimulants and Their Effects on a Viral Infection in Zebrafish (Danio rerio)
Subject:        In recent years, the innate immune response has gained importance since evidence indicates that after an adequate priming protocol, it is possible to obtain some prolonged and enhanced immune responses.
Keywords:       zebrafish, immunostimulants, β-glucans, stress, tolerance, IFN-γ, kynurenine, TDO
Author:         Beatriz Novoa
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     Item: 
      Title: Analysis of the Long-Lived Responses Induced by Immunostimulants and Their Effects on a Viral Infection in Zebrafish (Danio rerio)
      Children: 
       Item: 
        Title: Introduction
       Item: 
        Title: Materials and Methods
        Children: 
         Item: 
          Title: Fish, Viruses, and Bacteria
         Item: 
          Title: Plasmid Construction
         Item: 
          Title: Reagents
         Item: 
          Title: Stimulation With PAMPs and Challenge With SVCV
         Item: 
          Title: RNA Isolation and cDNA Transcription
         Item: 
          Title: Microarray Analysis
         Item: 
          Title: Microarray Validation and qPCR
         Item: 
          Title: Panther and Blast2GO Transcriptome Analysis
         Item: 
          Title: Overexpression of ifng1-2 in Zebrafish Larvae
         Item: 
          Title: Effect of Ifng1-2 and TDO and IDO Inhibitors on Mortality After an SVCV Challenge in Adult Zebrafish
         Item: 
          Title: Statistical Analyses
       Item: 
        Title: Results
        Children: 
         Item: 
          Title: Effect of Different PAMPs on the Zebrafish Transcriptome After 35 Days
         Item: 
          Title: Differential Responses to SVCV Challenge After Previous Administration of Different PAMPs and Effects on Survival
         Item: 
          Title: The Modulated Transcriptome of β-Glucan-Treated Fish at 35 Days Poststimulation Suggests an Immunosupressive State
         Item: 
          Title: β-Glucans Modify Lipid Metabolism by Inhibiting the Synthesis of Fatty Acids and Cholesterol Before and After Viral Infection
         Item: 
          Title: Effect of Ifng1-2 on Macrophage Activation and Survival After an SVCV Challenge
         Item: 
          Title: The Alteration of the Tryptophan–Kynurenine Pathway Influences the Survival Rate After SVCV Infection
         Item: 
          Title: Validation of the Microarray Data by qPCR
       Item: 
        Title: Discussion
       Item: 
        Title: Ethics Statement
       Item: 
        Title: Author Contributions
       Item: 
        Title: Acknowledgments
       Item: 
        Title: Funding
       Item: 
        Title: Supplementary Material
       Item: 
        Title: References
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   Type: MD5
  Checksum: e8a73ac1d311058b0081a9069ca499598e148720
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