910abf91cf59732b5da429afba7741dc pmc_30111840.pdf 910839a3587b7bc7ad3b35590f3c8c316a3515de pmc_30111840.pdf 4d0e7922068911bc5d77fffbcc07a6956f5b52db9219440f3cb10cf7673921bd pmc_30111840.pdf Title: Autism-like phenotype and risk gene-RNA deadenylation by CPEB4 mis-splicing Author: Alberto Parras, Héctor Anta, María Santos-Galindo, Vivek Swarup, Ainara Elorza, José L. Nieto-Gonzalez, Sara Picó, Ivó H. Hernández, Juan I. Díaz-Hernández, Eulàlia Belloc, Annie Rodolosse, Neelroop N. Parikshak, Olga Peñagarikano, Rafael Fernández-Chacón, Manuel Irimia, Pilar Navarro, Daniel H. Geschwind, Raúl Méndez, José J. Lucas Creator: AH XSL Formatter V6.3 R1 for Linux64 : 6.3.1.23495 (2016/02/03 14:39JST) Producer: Antenna House PDF Output Library 6.3.752 (Linux64) CreationDate: Mon Nov 5 21:42:58 2018 CET ModDate: Mon Nov 5 21:42:58 2018 CET Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 45 Encrypted: no Page size: 612 x 792 pts (letter) Page rot: 0 File size: 6290687 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- Times-Roman Type 1 Custom no no yes 11 0 Helvetica-Bold Type 1 Custom no no yes 13 0 Helvetica Type 1 Custom no no yes 16 0 Times-Bold Type 1 Custom no no yes 65 0 Helvetica-BoldOblique Type 1 Standard no no no 73 0 QVHNNJ+Code2000 CID TrueType Identity-H yes yes yes 123 0 Courier Type 1 Standard no no no 315 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-01-19 07:09:09 CET RepresentationInformation: pmc_30111840.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2020-07-06 04:17:50 CEST Size: 6290687 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Tagged PDF PDFMetadata: Objects: 0 FreeObjects: 137 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: true NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Abstract Item: Title: ASD-risk gene mRNAs bear CPEs and bind CPEB4 Item: Title: CPEB4 alteration in idiopathic ASD brains Item: Title: Deadenylation of ASD gene mRNAs in ASD brains Item: Title: ASD-like poly(A) changes in TgCPEB4Δ4 mice Item: Title: ASD-related phenotypes of TgCPEB4Δ4 mice Item: Title: Methods Children: Item: Title: Human brain tissue samples Item: Title: Animals Item: Title: RNA immunoprecipitation (RIP) Item: Title: RNA isolation, Whole Transcriptome Amplification (WTA) and microarrays processing Item: Title: Enrichment and co-expression network analysis of CPEB4 and CPEB1 binders Item: Title: Analysis of canonical and functional CPE sequences Item: Title: Simulations Item: Title: Quantification of CPEBs gene expression in human postmortem ASD cases Item: Title: Quantification of CPEBs transcript splicing and differential splicing analysis Item: Title: RNA extraction and cDNA synthesis Item: Title: CPEB4 PCR flanking primers Item: Title: Digital-Droplet PCR Item: Title: Real-time quantitative reverse transcriptase-PCR Item: Title: Western blot Children: Item: Title: Antibodies Item: Title: Poly(U) chromatography Item: Title: Human PrimeView and GeneAtlas MG-430 PM microarrays analysis Children: Item: Title: Differential gene expression array Item: Title: High-Resolution poly(A) tail (HIRE-PAT) assay Item: Title: Gene Ontology analysis Item: Title: Human-mouse altered poly(A) tail length geneset comparison Item: Title: Generation of TgCPEB4∆4 mice Item: Title: Immunohistochemistry Children: Item: Title: Antibodies Item: Title: Golgi spine analysis Item: Title: Brain weight and volumetric analysis Item: Title: Electrophysiology Children: Item: Title: Recordings Item: Title: Cleaved caspase-3 quantification Item: Title: Stereology Item: Title: Behavioral testing Children: Item: Title: Open Field Item: Title: Ultrasonic Vocalization (UsV) Item: Title: Social Approach Item: Title: Elevated plus maze (EPM) Item: Title: Data analysis Item: Title: Extended Data Item: Title: Extended Data Fig. 1 Item: Title: Extended Data Fig. 2 Item: Title: Extended Data Fig. 3 Item: Title: Extended Data Fig. 4 Item: Title: Extended Data Fig. 5 Item: Title: Extended Data Fig. 6 Item: Title: Extended Data Fig. 7 Item: Title: Extended Data Fig. 8 Item: Title: Extended Data Fig. 9 Item: Title: References Item: Title: Fig. 1 Item: Title: Fig. 2 Item: Title: Fig. 3 Item: Title: Fig. 4 Item: Title: Fig. 5 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1944 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1800 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1984 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1760 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2032 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2096 ImageHeight: 2085 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2096 ImageHeight: 1233 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2096 ImageHeight: 1407 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1864 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2096 ImageHeight: 759 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1856 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1176 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1384 ImageHeight: 2100 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1608 ImageHeight: 2099 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png ImageWidth: 16 ImageHeight: 16 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Fonts: Type0: Font: BaseFont: QVHNNJ+Code2000 Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: Helvetica FirstChar: 32 LastChar: 133 FontDescriptor: FontName: Helvetica Flags: Nonsymbolic FontBBox: -166, -225, 1000, 931 EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: Times-Bold FirstChar: 32 LastChar: 128 FontDescriptor: FontName: Times-Bold Flags: Nonsymbolic FontBBox: -168, -218, 1000, 935 EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: Helvetica-BoldOblique Font: BaseFont: Times-Roman FirstChar: 32 LastChar: 150 FontDescriptor: FontName: Times-Roman Flags: Nonsymbolic FontBBox: -168, -218, 1000, 898 EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: Courier Font: BaseFont: Helvetica-Bold FirstChar: 32 LastChar: 137 FontDescriptor: FontName: Helvetica-Bold Flags: Nonsymbolic FontBBox: -170, -228, 1003, 962 EncodingDictionary: Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: QVHNNJ+Code2000 CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: QVHNNJ+Code2000 Flags: Symbolic FontBBox: -1111, -295, 2107, 977 FontFile2: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 230, 197, 450, 205 LastModified: D:20181105154258-05'00' Page: Sequence: 2 Page: Sequence: 3 Page: Sequence: 4 Page: Sequence: 5 Page: Sequence: 6 Page: Sequence: 7 Page: Sequence: 8 Page: Sequence: 9 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 190, 174, 290, 184 LastModified: D:20181105154259-05'00' Annotation: Subtype: Link Rect: 214, 146, 319, 156 LastModified: D:20181105154259-05'00' Annotation: Subtype: Link Rect: 234, 132, 412, 142 LastModified: D:20181105154259-05'00' Annotation: Subtype: Link Rect: 438, 104, 466, 114 LastModified: D:20181105154259-05'00' Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 150, 720, 287, 730 LastModified: D:20181105154259-05'00' Annotation: Subtype: Link Rect: 201, 130, 357, 140 LastModified: D:20181105154259-05'00' Page: Sequence: 12 Page: Sequence: 13 Page: Sequence: 14 Page: Sequence: 15 Page: Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 440, 692, 468, 702 LastModified: D:20181105154259-05'00' Annotation: Subtype: Link Rect: 150, 678, 313, 688 LastModified: D:20181105154259-05'00' Page: Sequence: 17 Annotations: Annotation: Subtype: Link Rect: 188, 706, 321, 716 LastModified: D:20181105154259-05'00' Page: Sequence: 18 Page: Sequence: 19 Page: Sequence: 20 Page: Sequence: 21 Page: Sequence: 22 Page: Sequence: 23 Page: Sequence: 24 Page: Sequence: 25 Page: Sequence: 26 Page: Sequence: 27 Page: Sequence: 28 Page: Sequence: 29 Page: Sequence: 30 Page: Sequence: 31 Page: Sequence: 32 Page: Sequence: 33 Page: Sequence: 34 Page: Sequence: 35 Page: Sequence: 36 Page: Sequence: 37 Page: Sequence: 38 Page: Sequence: 39 Page: Sequence: 40 Page: Sequence: 41 Page: Sequence: 42 Page: Sequence: 43 Page: Sequence: 44 Page: Sequence: 45 Checksum: 369cdc6a Type: CRC32 Checksum: 910abf91cf59732b5da429afba7741dc Type: MD5 Checksum: 910839a3587b7bc7ad3b35590f3c8c316a3515de Type: SHA-1 Checksum: 4d0e7922068911bc5d77fffbcc07a6956f5b52db9219440f3cb10cf7673921bd Type: SHA-256