e17cbe739fcbedd795e93487b00605fc pmc_30143657.pdf 5f569a61b9b12eb7b6f1ff0bebfddb8f55afa795 pmc_30143657.pdf 1b563237c825e82cc2cf8a2dd1906f3c29a343c43483e4b65d8fe166d1f6db12 pmc_30143657.pdf Title: Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions Subject: Scientific Reports, doi:10.1038/s41598-018-30932-6 Keywords: Author: Daniel Crespo-Piazuelo Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Fri Aug 17 20:03:48 2018 CEST ModDate: Mon Aug 20 12:53:07 2018 CEST Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 3966756 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZNWREA+Corbel CID TrueType Identity-H yes yes yes 450 0 OCGJTG+Corbel-BoldItalic Type 1C WinAnsi yes yes yes 451 0 CEAURE+Corbel-Bold2 Type 1C WinAnsi yes yes yes 54 0 KKMJHS+Corbel2 Type 1C WinAnsi yes yes yes 56 0 KOEFCE+Corbel-Bold CID TrueType Identity-H yes yes yes 57 0 MinionPro-Regular Type 1C Custom yes no yes 58 0 GOYXYM+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 452 0 CEAURE+Corbel-Bold2 Type 1C WinAnsi yes yes yes 342 0 KKMJHS+Corbel2 Type 1C WinAnsi yes yes yes 343 0 ZNWREA+Corbel CID TrueType Identity-H yes yes yes 485 0 MyriadPro-It Type 1C WinAnsi yes no yes 345 0 MinionPro-It Type 1C Custom yes no yes 53 0 EuclidSymbol Type 1C Custom yes no yes 55 0 ZNWREA+Corbel CID TrueType Identity-H yes yes yes 344 0 MinionPro-Bold Type 1C WinAnsi yes no yes 75 0 CEAURE+Corbel-Bold2 Type 1C WinAnsi yes yes yes 355 0 KKMJHS+Corbel2 Type 1C WinAnsi yes yes yes 356 0 ZNWREA+Corbel CID TrueType Identity-H yes yes yes 357 0 PFHKUU+STIXGeneral-Regular Type 1C Custom yes yes yes 306 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-10-18 02:34:10 CEST RepresentationInformation: pmc_30143657.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-02-05 11:43:52 CET Size: 3966756 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 1. (a) Stacked area plot of the OTUs grouped by phyla for the 65 samples sorted by intestinal section. (b) Percentage evolution along the gut of the ten most abundant bacterial genera in the dataset. Segments represent the standard er:53' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 1. (a) Stacked area plot of the OTUs grouped by phyla for the 65 samples sorted by intestinal section. (b) Percentage evolution along the gut of the ten most abundant bacterial genera in the dataset. Segments represent the standard e:54' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal section. (:55' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal section. :56' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal section.:57' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 3. Five-part Venn diagram for each of the 13 subjects, showing the OTUs shared among the intestinal sections: duodenum (red), jejunum (yellow), ileum (green), proximal colon (blue), and distal colon (purple). The numbers in the diagrams r:59' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 4. SparCC55 PCIT56 (SPCIT13) prokaryotic genus network for partial correlations with an absolute value above 0.65 between log-transformed genus abundances performed in the (a) duodenum, (b) jejunum, (c) ileum, (d) prox:61' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 4. SparCC55 PCIT56 (SPCIT13) prokaryotic genus network for partial correlations with an absolute value above 0.65 between log-transformed genus abundances performed in the (a) duodenum, (b) jejunum, (c) ileum, (d) pro:65' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 4. SparCC55 PCIT56 (SPCIT13) prokaryotic genus network for partial correlations with an absolute value above 0.65 between log-transformed genus abundances performed in the (a) duodenum, (b) jejunum, (c) ileum, (d) pr:66' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 4. SparCC55 PCIT56 (SPCIT13) prokaryotic genus network for partial correlations with an absolute value above 0.65 between log-transformed genus abundances performed in the (a) duodenum, (b) jejunum, (c) ileum, (d) :68' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 4. SparCC55 PCIT56 (SPCIT13) prokaryotic genus network for partial correlations with an absolute value above 0.65 between log-transformed genus abundances performed in the (a) duodenum, (b) jejunum, (c) ileum, (d):69' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 5. Non-metric multidimensional scaling (NMDS) plot based on Bray-Curtis dissimilarities for the metagenome (KEGG26 orthologies (KOs) counts) predicted through PICRUSt25 for the 65 samples from the 13 pigs in each of the 5 intestin:73' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal sectio:74' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 5. Non-metric multidimensional scaling (NMDS) plot based on Bray-Curtis dissimilarities for the metagenome (KEGG26 orthologies (KOs) counts) predicted through PICRUSt25 for the 65 samples from the 13 pigs in each of the 5 intesti:75' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal secti:79' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal sect:82' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal sec:84' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 1. (a) Stacked area plot of the OTUs grouped by phyla for the 65 samples sorted by intestinal section. (b) Percentage evolution along the gut of the ten most abundant bacterial genera in the dataset. Segments represent the standard :85' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 2. Descriptive plots made from the OTUs obtained in each sample. (a) Boxplot of the Shannon α-diversity for the 13 pigs in each intestinal section. (b) Boxplot of the Whittaker β-diversity for the 13 pigs in each intestinal se:86' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 1. (a) Stacked area plot of the OTUs grouped by phyla for the 65 samples sorted by intestinal section. (b) Percentage evolution along the gut of the ten most abundant bacterial genera in the dataset. Segments represent the standard:93' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd: 17. Zhao, W. et al. The dynamic distribution of porcine microbiota across different ages and gastrointestinal tract segments. PLoS One 10, 1–13 (2015). :104' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 1. (a) Stacked area plot of the OTUs grouped by phyla for the 65 samples sorted by intestinal section. (b) Percentage evolution along the gut of the ten most abundant bacterial genera in the dataset. Segments represent the standar:110' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd:Figure 1. (a) Stacked area plot of the OTUs grouped by phyla for the 65 samples sorted by intestinal section. (b) Percentage evolution along the gut of the ten most abundant bacterial genera in the dataset. Segments represent the standa:127' cannot be found Offset: 3575851 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_30932.indd: 13. Ramayo-Caldas, Y. et al. Phylogenetic network analysis applied to pig gut microbiota identifies an ecosystem structure linked with growth traits. ISME J. 10, 2973–2977 (2016). :153' cannot be found Offset: 3575851 MIMEtype: application/pdf PDFMetadata: Objects: 531 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functio ... Children: Item: Title: Results Children: Item: Title: Microbial taxonomic composition shows major differences along the intestine. Item: Title: Microbiota interaction network reconstruction among the gut sections. Item: Title: Presence/absence and differential abundance analysis of genera between consecutive sections. Item: Title: Functional analysis of the gut metagenome along the intestine. Item: Title: Discussion Item: Title: Methods Children: Item: Title: Ethics statement. Item: Title: Animals and lumen content collection. Item: Title: DNA extraction and 16S rRNA gene sequencing. Item: Title: Taxonomic classification of the gut samples. Item: Title: Diversity studies and differences in the abundances of the gut microbiota. Item: Title: Metagenome prediction and functional differences amongst gut sections. Item: Title: Network prediction with the SPCIT approach. Item: Title: Acknowledgements Item: Title: Figure 1 (a) Stacked area plot of the OTUs grouped by phyla for the 65 samples sorted by intestinal section. Item: Title: Figure 2 Descriptive plots made from the OTUs obtained in each sample. Item: Title: Figure 3 Five-part Venn diagram for each of the 13 subjects, showing the OTUs shared among the intestinal sections: duodenum (red), jejunum (yellow), ileum (green), proximal colon (blue), and distal colon (purple). Item: Title: Figure 4 SparCC55 PCIT56 (SPCIT13) prokaryotic genus network for partial correlations with an absolute value above 0. Item: Title: Figure 5 Non-metric multidimensional scaling (NMDS) plot based on Bray-Curtis dissimilarities for the metagenome (KEGG26 orthologies (KOs) counts) predicted through PICRUSt25 for the 65 samples from the 13 pigs in each of the 5 intestinal sections (repres Info: Title: Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions Author: Daniel Crespo-Piazuelo Subject: Scientific Reports, doi:10.1038/s41598-018-30932-6 Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Fri Aug 17 20:33:48 CEST 2018 ModDate: Mon Aug 20 12:53:07 CEST 2018 ID: 0xf06c700f3aeb84b9e8c4c8b30499cafd, 0xc0c3833b60d90eb1b06d37cde601322d Filters: FilterPipeline: FlateDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1100 ImageHeight: 90 ColorSpace: black is zero BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X 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Daniel Crespo-Piazuelo Jordi Estellamp#x000E9; Manuel Revilla Lourdes Criado-Mesas Yuliaxis Ramayo-Caldas Cristina amp#x000D3;vilo Ana I. Fernamp#x000E1;ndez Maria Ballester Josep M. Folch 10.1038/s41598-018-30932-6 http://dx.doi.org/10.1038/s41598-018-30932-6 journal Scientific Reports © 2018, The Author(s) 2045-2322 10.1038/s41598-018-30932-6 Springer 2018-08-20T12:53:07+02:00 2018-08-17T23:33:48+05:30 2018-08-20T12:53:07+02:00 True iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) VoR uuid:12afa588-9ddc-4807-9caa-4e3dd5ef0ad2 uuid:d7d47664-2add-4721-b465-a379c17ab8fa default 1 converted uuid:cbdfcce3-a399-423b-a11f-a156fc64a66f converted to PDF/A-2b pdfToolbox 2018-08-17T23:34:25+05:30 2 B http://ns.adobe.com/xap/1.0/mm/ xmpMM XMP Media Management Schema internal UUID based identifier for specific incarnation of a document InstanceID URI internal The common identifier for all versions and renditions of a document. 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