add46d1dac6ae960fd07a1ef729fc23b pmc_29373502.pdf bf75164563aab68e721a21f59230bfacad1b1363 pmc_29373502.pdf 1afe12512baffa9f6b507a77ad4371d45e4b88f66514c088d4f7fb584bc48314 pmc_29373502.pdf Title: Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy Subject: This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by electrochemical grafting. The detection was performed using [Fe(CN)6]3-/[Fe(CN)6]4- as redox probe. After recording the frequency response, values were fitted to its electric model using the principle of equivalent circuits. The aptasensor showed a linear response up to 5 M for lysozyme and a limit of detection of 1.67 M. The sensitivity of the established method was 0.090 M-1 in relative charge transfer resistance values. The interference response by main proteins, such as bovine serum albumin and cytochrome c, has been also characterized. To finally verify the performance of the developed aptasensor, it was applied to wine analysis. Keywords: aptamer; biosensor; electrochemical impedance spectroscopy; electrochemical grafting Author: Dionisia Ortiz-Aguayo and Manel del Valle Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Jan 26 09:39:10 2018 CET ModDate: Fri Jan 26 09:39:10 2018 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2220998 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BOCKWD+URWPalladioL-Ital Type 1 Custom yes yes no 114 0 NDTJZN+URWPalladioL-Bold Type 1 Custom yes yes no 115 0 OXBKYU+TeXGyreAdventor-Bold Type 1 Custom yes yes no 116 0 BXGOKX+URWPalladioL-Roma Type 1 Custom yes yes no 117 0 LNTTSH+VnURWPalladioL Type 1 Custom yes yes no 118 0 DYVFWR+CMSY10 Type 1 Builtin yes yes no 119 0 QWWHCD+EURM10 Type 1 Builtin yes yes no 120 0 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yes 436 0 KCHCBI+PalatinoLinotype CID TrueType Identity-H yes yes yes 487 0 KCHCCJ+PalatinoLinotype TrueType WinAnsi yes yes no 488 0 KCHCGJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 489 0 KCHCGL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 490 0 KCHDBI+TimesNewRoman CID TrueType Identity-H yes yes yes 491 0 KCHCCJ+PalatinoLinotype TrueType WinAnsi yes yes no 549 0 KCHCGJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 550 0 KCHCGK+PalatinoLinotype,Bold CID TrueType Identity-H yes yes yes 551 0 KCHCGL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 552 0 KCHDBI+TimesNewRoman CID TrueType Identity-H yes yes yes 553 0 XIRVNA+EURB10 Type 1 Builtin yes yes no 610 0 PDXFNG+PazoMath-Bold Type 1 Builtin yes yes no 611 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-01-19 07:03:44 CET RepresentationInformation: pmc_29373502.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2018-03-26 03:50:17 CEST Size: 2220998 Format: PDF Version: 1.5 Status: Well-Formed 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