77e2a8b66d6ddeb2a87c594e4aefb009 pmc_29734798.pdf 77ac7f94638ddb9585032f55f26f66c84cbdde87 pmc_29734798.pdf Title: The Disordered C-Terminus of Yeast Hsf1 Contains a Cryptic Low-Complexity Amyloidogenic Region Subject: Response mechanisms to external stress rely on networks of proteins able to activate specific signaling pathways to ensure the maintenance of cell proteostasis. Many of the proteins mediating this kind of response contain intrinsically disordered regions, which lack a defined structure, but still are able to interact with a wide range of clients that modulate the protein function. Some of these interactions are mediated by specific short sequences embedded in the longer disordered regions. Because the physicochemical properties that promote functional and abnormal interactions are similar, it has been shown that, in globular proteins, aggregation-prone and binding regions tend to overlap. It could be that the same principle applies for disordered protein regions. In this context, we show here that a predicted low-complexity interacting region in the disordered C-terminus of the stress response master regulator heat shock factor 1 (Hsf1) protein corresponds to a cryptic amyloid region able to self-assemble into fibrillary structures resembling those found in neurodegenerative disorders. Keywords: amyloid; Hsf1; protein aggregation; intrinsically disordered regions; Q/N-rich regions; low complexity; molecular recognition features Author: Jordi Pujols, Jaime Santos, Irantzu Pallarès and Salvador Ventura Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Mon May 7 10:11:27 2018 CEST ModDate: Mon May 7 10:11:27 2018 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2717189 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HCLQQC+URWPalladioL-Ital Type 1 Custom yes yes no 103 0 QUGULU+URWPalladioL-Bold Type 1 Custom yes yes no 104 0 OXBKYU+TeXGyreAdventor-Bold Type 1 Custom yes yes no 105 0 WYEKQU+VnURWPalladioL-Bold Type 1 Custom yes yes no 106 0 IGGCGS+URWPalladioL-Roma Type 1 Custom yes yes no 107 0 DFFCNG+VnURWPalladioL Type 1 Custom yes yes no 108 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 113 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 114 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 129 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 130 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 131 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 219 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 220 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 221 0 Times New Roman TrueType WinAnsi no no no 222 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 223 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 263 0 VORBJF+CMSY10 Type 1 Builtin yes yes no 264 0 CRJHCU+PazoMath-Italic Type 1 Builtin yes yes no 265 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 269 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 270 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 271 0 Times New Roman TrueType WinAnsi no no no 272 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 273 0 ABCDEE+Palatino Linotype,Italic CID TrueType Identity-H yes yes yes 274 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 316 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 317 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 318 0 Times New Roman TrueType WinAnsi no no no 319 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 320 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 352 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 353 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 354 0 Times New Roman TrueType WinAnsi no no no 355 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 356 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 389 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 390 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 391 0 Times New Roman TrueType WinAnsi no no no 392 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 393 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 428 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 429 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 430 0 Times New Roman TrueType WinAnsi no no no 431 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 432 0 java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary at edu.harvard.hul.ois.jhove.module.PdfModule.readDocCatalogDict(PdfModule.java:1284) at edu.harvard.hul.ois.jhove.module.PdfModule.parse(PdfModule.java:525) at edu.harvard.hul.ois.jhove.JhoveBase.processFile(JhoveBase.java:825) at edu.harvard.hul.ois.jhove.JhoveBase.process(JhoveBase.java:614) at edu.harvard.hul.ois.jhove.JhoveBase.dispatch(JhoveBase.java:465) at Jhove.main(Jhove.java:296) Jhove (Rel. 1.6, 2011-01-04) Date: 2019-02-14 17:20:48 CET RepresentationInformation: pmc_29734798.pdf ReportingModule: BYTESTREAM, Rel. 1.3 (2007-04-10) LastModified: 2018-06-11 03:40:15 CEST Size: 2717189 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: 38c86798 Type: CRC32 Checksum: 77e2a8b66d6ddeb2a87c594e4aefb009 Type: MD5 Checksum: 77ac7f94638ddb9585032f55f26f66c84cbdde87 Type: SHA-1