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Offset: 6449342 MIMEtype: application/pdf PDFMetadata: Objects: 874 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: ATR is required to complete meiotic recombination in mice Destination: title_link Children: Item: Title: Results Destination: A1 Children: Item: Title: Using the Seckel mouse model to study meiotic ATR functions Destination: B1 Item: Title: Modest recombination defects of Seckel mouse spermatocytes Destination: B2 Item: Title: ATR inhibition in’vivo impairs meiotic recombination Destination: B3 Item: Title: In vitro ATR inhibition impairs meiotic prophase progression Destination: B4 Item: Title: CHK1 function is required for proper RAD51 loading Destination: B5 Item: Title: ATR promotes SC elongation Destination: B6 Item: Title: Accumulation of recombination markers on unsynapsed axes Destination: B7 Item: Title: Discussion Destination: A2 Item: Title: Methods Destination: A3 Children: Item: Title: Mice and genotyping Destination: B8 Item: Title: RNA expression analysis Destination: B9 Item: Title: Cytology, immunostaining, and FISH Destination: B10 Item: Title: RNA-FISH and immunofluorescence Destination: B11 Item: Title: SPO11-oligonucleotide complex detection and western blotting Destination: B12 Item: Title: In vivo drug administration Destination: B13 Item: Title: Neonatal testis organ culture Destination: B14 Item: Title: SC length, MLH1 distribution, and recombination focus density Destination: B15 Item: Title: Statistical analysis Destination: B16 Item: Title: Image processing and data analysis Destination: B17 Item: Title: Data availability Destination: B18 Item: Title: References Destination: A4 Item: Title: Acknowledgements Destination: A5 Item: Title: Author contributions Destination: A6 Item: Title: Competing interests Destination: A7 Item: Title: ACKNOWLEDGEMENTS Destination: M1 Info: Title: ATR is required to complete meiotic recombination in mice 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