037bda6b4d872007ca50edc58dea3e44 pmc_30214484.pdf bff6431bd2ce04f4c8338aba89d692aa1e006091 pmc_30214484.pdf 0f24cf41003d7d9cee17501e543bc2caacbda290c7e5766d444f0f14312d568c pmc_30214484.pdf Title: Engineering and monitoring cellular barrier models Subject: Journal of Biological Engineering, 2018, doi:10.1186/s13036-018-0108-5 Keywords: Biological barriers,Cell barrier function,Microphysiological systems,Transepithelial electrical properties Author: Jose Yeste Creator: Arbortext Advanced Print Publisher 9.1.440/W Unicode Producer: Acrobat Distiller 10.1.5 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Wed Aug 15 02:08:22 2018 CEST ModDate: Wed Sep 12 06:57:53 2018 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 790.866 pts Page rot: 0 File size: 4570967 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MyriadPro-Regular Type 1 Custom yes no no 11 0 CBMHOM+HwlrvsAdvTT99c4c969 Type 1C WinAnsi yes yes no 14 0 CBMHLL+TvmkynAdvTTe45e47d2 Type 1C WinAnsi yes yes no 16 0 CBMHON+YyxslvAdvTT86d47313 Type 1C WinAnsi yes yes no 18 0 CBMHLM+WjbxfvAdvTTb5929f4c Type 1C WinAnsi yes yes no 12 0 CBMHPO+MkghwsAdvTT86d47313+20 Type 1C WinAnsi yes yes no 13 0 CBMILK+GxsgqtAdvTT7329fd89.I Type 1C WinAnsi yes yes no 17 0 CBMHJL+JwrhqnAdvTTaf7f9f4f.B Type 1C WinAnsi yes yes no 15 0 CBNDOI+MsgdxyAdvTT86d47313+03 Type 1C Custom yes yes yes 33 0 CBNEBI+RwggpdAdvTT83913201.I Type 1C WinAnsi yes yes no 88 0 CBNIPL+BmyjnxAdvTT86d47313+22 Type 1C Custom yes yes yes 100 0 CBNIPM+DvxmxtAdvTT8861b38f.I Type 1C WinAnsi yes yes no 101 0 CBNIPN+DhnwfyAdvP4C4E74 Type 1C WinAnsi yes yes no 102 0 CBNIPO+GwthhxAdvTT8861b38f.I+03 Type 1C Custom yes yes yes 103 0 CBNIPP+HkmhqgAdvP4C4E46 Type 1C Custom yes yes no 104 0 CBOAIC+HbfnpyAdvTTb5929f4c+20 Type 1C WinAnsi yes yes no 155 0 CBOAID+GwcqlbAdvTTb5929f4c+03 Type 1C Custom yes yes yes 156 0 CBOLAD+FnmdrtAdvP4C4E51 Type 1C WinAnsi yes yes no 201 0 CBPOKL+FrnlhmAdvTT99c4c969+20 Type 1C WinAnsi yes yes no 376 0 CBPOLM+MlwdmgAdvTTaf7f9f4f.B+20 Type 1C WinAnsi yes yes no 377 0 CBPONM+JwfrdqAdvTTb5929f4c+01 Type 1C Custom yes yes yes 386 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-01-19 06:18:04 CET RepresentationInformation: pmc_30214484.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2020-07-06 04:17:32 CEST Size: 4570967 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 783 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Abstract Destination: sectionAbs1 Item: Title: Background Destination: sectionSec1 Item: Title: Epithelial and endothelial tissues Destination: sectionSec2 Children: Item: Title: Barrier function Destination: sectionSec3 Item: Title: Cell microenvironment Destination: sectionSec4 Children: Item: Title: Extracellular matrix (ECM) Destination: sectionSec5 Item: Title: Cell-cell communication Destination: sectionSec6 Item: Title: Biochemical cues Destination: sectionSec7 Item: Title: Physical cues Destination: sectionSec8 Item: Title: Barrier function quantification Destination: sectionSec9 Children: Item: Title: Permeability assays Destination: sectionSec10 Item: Title: Transepithelial electrical measurements Destination: sectionSec11 Children: Item: Title: Transepithelial voltage Destination: sectionSec12 Item: Title: Transepithelial electrical resistance Destination: sectionSec13 Item: Title: Short-circuit current Destination: sectionSec14 Item: Title: Equivalent electric circuit and impedance analysis Destination: sectionSec15 Item: Title: Measurement techniques Destination: sectionSec16 Children: Item: Title: Ussing chamber Destination: sectionSec17 Item: Title: Transwells Destination: sectionSec18 Item: Title: ECIS Destination: sectionSec19 Item: Title: Engineered biological barrier models Destination: sectionSec20 Children: Item: Title: Membrane-based devices Destination: sectionSec21 Item: Title: Microchannels Destination: sectionSec22 Item: Title: Gel-liquid interface Destination: sectionSec23 Item: Title: Tubules and vessels embedded within an ECM Destination: sectionSec24 Item: Title: Conclusions and future perspectives Destination: sectionSec25 Item: Title: Abbreviations Destination: sectionabr1 Item: Title: Acknowledgments Destination: section1 Item: Title: Funding Destination: sectionFPar1 Item: Title: Authors’ contributions Destination: section2 Item: Title: Ethics approval and consent to participate Destination: sectionFPar2 Item: Title: Consent for publication Destination: sectionFPar3 Item: Title: Competing interests Destination: sectionFPar4 Item: Title: Publisher’s Note Destination: sectionFPar5 Item: Title: References Destination: sectionBib1 Info: Title: Engineering and monitoring cellular barrier models Author: Jose Yeste Subject: Journal of Biological Engineering, 2018, doi:10.1186/s13036-018-0108-5 Keywords: Biological barriers,Cell barrier function,Microphysiological systems,Transepithelial electrical properties Creator: Arbortext Advanced Print Publisher 9.1.440/W Unicode Producer: Acrobat Distiller 10.1.5 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Wed Aug 15 02:08:22 CEST 2018 ModDate: Wed Sep 12 06:57:53 CEST 2018 ID: 0xdd9ffefc7747c7c58982228a993af6cf, 0xdb88951433f11281269066a1254e4bb0 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 326 ImageHeight: 87 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 688 ImageHeight: 126 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 95 ImageHeight: 91 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 54 ImageHeight: 103 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 176 ImageHeight: 182 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1949 ImageHeight: 1162 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1420 ImageHeight: 1137 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1420 ImageHeight: 1152 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: Perceptual Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1420 ImageHeight: 1069 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1183 ImageHeight: 774 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: Perceptual Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1420 ImageHeight: 1661 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1420 ImageHeight: 1661 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1100 ImageHeight: 90 ColorSpace: black is zero BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode Fonts: Type1: Font: BaseFont: CBNDOI+MsgdxyAdvTT86d47313+03 FontSubset: true FirstChar: 1 LastChar: 7 FontDescriptor: FontName: CBNDOI+MsgdxyAdvTT86d47313+03 Flags: Symbolic FontBBox: -281, -250, 906, 885 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: CBPONM+JwfrdqAdvTTb5929f4c+01 FontSubset: true FirstChar: 1 LastChar: 158 FontDescriptor: FontName: CBPONM+JwfrdqAdvTTb5929f4c+01 Flags: Symbolic FontBBox: -41, -218, 1114, 1062 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: CBNIPL+BmyjnxAdvTT86d47313+22 FontSubset: true FirstChar: 2 LastChar: 22 FontDescriptor: FontName: CBNIPL+BmyjnxAdvTT86d47313+22 Flags: Symbolic FontBBox: -166, -260, 1031, 906 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: CBNIPM+DvxmxtAdvTT8861b38f.I FontSubset: true FirstChar: 32 LastChar: 116 FontDescriptor: FontName: CBNIPM+DvxmxtAdvTT8861b38f.I Flags: Nonsymbolic FontBBox: -187, -239, 1062, 875 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBNIPN+DhnwfyAdvP4C4E74 FontSubset: true FirstChar: 32 LastChar: 240 FontDescriptor: FontName: CBNIPN+DhnwfyAdvP4C4E74 Flags: Nonsymbolic FontBBox: -20, -958, 1114, 770 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBNIPO+GwthhxAdvTT8861b38f.I+03 FontSubset: true FirstChar: 1 LastChar: 2 FontDescriptor: FontName: CBNIPO+GwthhxAdvTT8861b38f.I+03 Flags: Symbolic FontBBox: -291, -239, 937, 885 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: CBNIPP+HkmhqgAdvP4C4E46 FontSubset: true FirstChar: 1 LastChar: 4 FontDescriptor: FontName: CBNIPP+HkmhqgAdvP4C4E46 Flags: Symbolic FontBBox: 207, -2359, 700, 40 FontFile3: true EncodingDictionary: Differences: true Font: BaseFont: CBOLAD+FnmdrtAdvP4C4E51 FontSubset: true FirstChar: 32 LastChar: 59 FontDescriptor: FontName: CBOLAD+FnmdrtAdvP4C4E51 Flags: Nonsymbolic FontBBox: -31, -239, 1041, 750 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: MyriadPro-Regular FirstChar: 1 LastChar: 255 FontDescriptor: FontName: MyriadPro-Regular Flags: Nonsymbolic FontBBox: -46, -250, 1126, 879 FontFile: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: CBMHLM+WjbxfvAdvTTb5929f4c FontSubset: true FirstChar: 32 LastChar: 252 FontDescriptor: FontName: CBMHLM+WjbxfvAdvTTb5929f4c Flags: Nonsymbolic FontBBox: -41, -250, 812, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBMHPO+MkghwsAdvTT86d47313+20 FontSubset: true FirstChar: 32 LastChar: 151 FontDescriptor: FontName: CBMHPO+MkghwsAdvTT86d47313+20 Flags: Nonsymbolic FontBBox: -218, -218, 1291, 812 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBMHOM+HwlrvsAdvTT99c4c969 FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: CBMHOM+HwlrvsAdvTT99c4c969 Flags: Nonsymbolic FontBBox: -41, -250, 854, 885 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBMHJL+JwrhqnAdvTTaf7f9f4f.B FontSubset: true FirstChar: 32 LastChar: 118 FontDescriptor: FontName: CBMHJL+JwrhqnAdvTTaf7f9f4f.B Flags: Nonsymbolic FontBBox: -218, -250, 875, 895 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBMHLL+TvmkynAdvTTe45e47d2 FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: CBMHLL+TvmkynAdvTTe45e47d2 Flags: Nonsymbolic FontBBox: -218, -250, 833, 875 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBMILK+GxsgqtAdvTT7329fd89.I FontSubset: true FirstChar: 32 LastChar: 117 FontDescriptor: FontName: CBMILK+GxsgqtAdvTT7329fd89.I Flags: Nonsymbolic FontBBox: -218, -250, 885, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBMHON+YyxslvAdvTT86d47313 FontSubset: true FirstChar: 32 LastChar: 126 FontDescriptor: FontName: CBMHON+YyxslvAdvTT86d47313 Flags: Serif, Nonsymbolic FontBBox: -218, -239, 1020, 875 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBNEBI+RwggpdAdvTT83913201.I FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: CBNEBI+RwggpdAdvTT83913201.I Flags: Nonsymbolic FontBBox: -93, -250, 906, 875 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBPOKL+FrnlhmAdvTT99c4c969+20 FontSubset: true FirstChar: 32 LastChar: 146 FontDescriptor: FontName: CBPOKL+FrnlhmAdvTT99c4c969+20 Flags: Nonsymbolic FontBBox: -447, -197, 1583, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBPOLM+MlwdmgAdvTTaf7f9f4f.B+20 FontSubset: true FirstChar: 32 LastChar: 146 FontDescriptor: FontName: CBPOLM+MlwdmgAdvTTaf7f9f4f.B+20 Flags: Nonsymbolic FontBBox: -468, -197, 1656, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBOAIC+HbfnpyAdvTTb5929f4c+20 FontSubset: true FirstChar: 32 LastChar: 150 FontDescriptor: FontName: CBOAIC+HbfnpyAdvTTb5929f4c+20 Flags: Nonsymbolic FontBBox: -447, -208, 1375, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: CBOAID+GwcqlbAdvTTb5929f4c+03 FontSubset: true FirstChar: 1 LastChar: 6 FontDescriptor: FontName: CBOAID+GwcqlbAdvTTb5929f4c+03 Flags: Symbolic FontBBox: -531, -260, 802, 968 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true XMP: Biological barriers,Cell barrier function,Microphysiological systems,Transepithelial electrical properties Acrobat Distiller 10.1.5 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) application/pdf 10.1186/s13036-018-0108-5 Journal of Biological Engineering Journal of Biological Engineering, 2018, doi:10.1186/s13036-018-0108-5 Biological barriers Cell barrier function Microphysiological systems Transepithelial electrical properties Engineering and monitoring cellular barrier models Jose Yeste Xavi Illa Mar Alvarez Rosa Villa 2018-09-12T06:57:53+02:00 2018-08-15T08:08:22+08:00 Arbortext Advanced Print Publisher 9.1.440/W Unicode 2018-09-12T06:57:53+02:00 uuid:a1b7c7de-c10d-45a8-aaf3-dc26a13a47f4 uuid:5d929e01-b896-4da2-bc19-2346f569513b default 1 converted uuid:2ac1e082-0d1f-454e-9a68-c1ee319cd490 converted to PDF/A-2b pdfToolbox 2018-08-15T08:15:09+08:00 2 B Jose Yeste http://orcid.org/0000-0001-7540-7305 Springer Nature ORCID Schema http://springernature.com/ns/xmpExtensions/2.0/ sn authorInfo Bag AuthorInformation external Author information: contains the name of each author and his/her ORCID (ORCiD: Open Researcher and Contributor ID). An ORCID is a persistent identifier (a non-proprietary alphanumeric code) to uniquely identify scientific and other academic authors. editorInfo Bag EditorInformation external Editor information: contains the name of each editor and his/her ORCID identifier. seriesEditorInfo Bag SeriesEditorInformation external Series editor information: contains the name of each series editor and his/her ORCID identifier. AuthorInformation http://springernature.com/ns/xmpExtensions/2.0/authorInfo/ author Specifies the types of author information: name and ORCID of an author. name Text Gives the name of an author. orcid URI Gives the ORCID of an author. EditorInformation http://springernature.com/ns/xmpExtensions/2.0/editorInfo/ editor Specifies the types of editor information: name and ORCID of an editor. name Text Gives the name of an editor. orcid URI Gives the ORCID of an editor. SeriesEditorInformation http://springernature.com/ns/xmpExtensions/2.0/seriesEditorInfo/ seriesEditor Specifies the types of series editor information: name and ORCID of a series editor. name Text Gives the name of a series editor. orcid URI Gives the ORCID of a series editor. http://ns.adobe.com/pdf/1.3/ pdf Adobe PDF Schema internal A name object indicating whether the document has been modified to include trapping information Trapped Text http://ns.adobe.com/xap/1.0/mm/ xmpMM XMP Media Management Schema internal UUID based identifier for specific incarnation of a document InstanceID URI internal The common identifier for all versions and renditions of a document. OriginalDocumentID URI internal A reference to the original document from which this one is derived. It is a minimal reference; missing components can be assumed to be unchanged. For example, a new version might only need to specify the instance ID and version number of the previous version, or a rendition might only need to specify the instance ID and rendition class of the original. DerivedFrom ResourceRef Identifies a portion of a document. This can be a position at which the document has been changed since the most recent event history (stEvt:changed). For a resource within an xmpMM:Ingredients list, the ResourceRef uses this type to identify both the portion of the containing document that refers to the resource, and the portion of the referenced resource that is referenced. http://ns.adobe.com/xap/1.0/sType/Part# stPart Part http://ns.adobe.com/xap/1.0/t/pg/ xmpTPg XMP Paged-Text internal A structure containing the characteristics of a font used in a document Fonts Bag Font internal XMP08 Spec: An ordered array of plate names that are needed to print the document (including any in contained documents). PlateNames Seq Text A structure containing the characteristics of a font used in a document. http://ns.adobe.com/xap/1.0/sType/Font# stFnt Font http://ns.adobe.com/pdfx/1.3/ pdfx PDF/X ID Schema internal ID of PDF/X standard GTS_PDFXVersion Text internal Conformance level of PDF/X standard GTS_PDFXConformance Text internal Company creating the PDF Company Text internal Date when document was last modified SourceModified Text http://www.aiim.org/pdfa/ns/id/ pdfaid PDF/A ID Schema internal Part of PDF/A standard part Integer internal Amendment of PDF/A standard amd Text internal Conformance level of PDF/A standard conformance Text Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 484, 657, 538, 679 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 111, 599, 120, 608 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 280, 326, 285, 336 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 190, 182, 195, 192 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 201, 182, 206, 192 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 236, 146, 241, 156 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 249, 146, 254, 156 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 307, 290, 312, 300 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 307, 278, 312, 288 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 356, 278, 360, 288 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 417, 278, 427, 288 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 523, 230, 533, 240 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 114, 126, 166, 134 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 239, 65, 357, 72 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 182, 41, 321, 48 Flags: Print AppearanceDictionary: true Thumb: true Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 204, 537, 214, 546 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 220, 537, 229, 546 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 324, 597, 333, 606 Flags: Print AppearanceDictionary: true Destination: 3 Annotation: Subtype: Link Rect: 467, 441, 477, 450 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 332, 417, 341, 426 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 166, 63, 169, 71 Flags: Print AppearanceDictionary: true Destination: 383 Thumb: true Page: Label: 3 Annotations: Annotation: Subtype: Link Rect: 146, 681, 155, 690 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 97, 633, 107, 642 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 231, 573, 241, 582 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 237, 513, 246, 522 Flags: Print AppearanceDictionary: true Destination: 3 Annotation: Subtype: Link Rect: 252, 513, 257, 522 Flags: Print AppearanceDictionary: true Destination: 3 Annotation: Subtype: Link Rect: 95, 405, 105, 414 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 109, 405, 119, 414 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 234, 393, 244, 402 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 97, 321, 107, 330 Flags: Print AppearanceDictionary: true Destination: 3 Annotation: Subtype: Link Rect: 162, 249, 172, 258 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 217, 201, 227, 210 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 233, 201, 243, 210 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 88, 57, 93, 66 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 449, 681, 458, 690 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 463, 681, 472, 690 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 523, 573, 533, 582 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 496, 549, 506, 558 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 511, 549, 521, 558 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 307, 525, 317, 534 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 372, 345, 382, 354 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 451, 297, 460, 306 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 465, 297, 474, 306 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 508, 273, 517, 282 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 512, 225, 522, 234 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 307, 177, 317, 186 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 503, 105, 512, 114 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 449, 57, 454, 66 Flags: Print AppearanceDictionary: true Destination: 85 Thumb: true Page: Label: 4 Annotations: Annotation: Subtype: Link Rect: 59, 549, 69, 558 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 259, 417, 269, 426 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 275, 417, 285, 426 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 420, 597, 430, 606 Flags: Print AppearanceDictionary: true Destination: 383 Thumb: true Page: Label: 5 Annotations: Annotation: Subtype: Link Rect: 140, 657, 150, 666 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 137, 645, 147, 654 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 117, 621, 127, 630 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 132, 621, 142, 630 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 135, 477, 145, 486 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 151, 477, 161, 486 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 160, 417, 170, 426 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 59, 381, 69, 390 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 76, 381, 86, 390 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 165, 345, 175, 354 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 180, 345, 189, 354 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 59, 249, 69, 258 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 94, 213, 104, 222 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 118, 189, 128, 198 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 179, 81, 189, 90 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 196, 81, 205, 90 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 243, 57, 253, 66 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 425, 477, 435, 486 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 307, 194, 317, 203 Flags: Print AppearanceDictionary: true Destination: 390 Thumb: true Page: Label: 6 Annotations: Annotation: Subtype: Link Rect: 224, 585, 234, 594 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 129, 513, 139, 522 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 130, 477, 140, 486 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 145, 477, 154, 486 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 453, 441, 463, 450 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 342, 429, 352, 438 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 349, 381, 358, 390 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 461, 345, 471, 354 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 434, 93, 444, 102 Flags: Print AppearanceDictionary: true Destination: 152 Thumb: true Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 253, 645, 264, 654 Flags: Print AppearanceDictionary: true Destination: 152 Annotation: Subtype: Link Rect: 191, 609, 201, 618 Flags: Print AppearanceDictionary: true Destination: 152 Annotation: Subtype: Link Rect: 123, 585, 133, 594 Flags: Print AppearanceDictionary: true Destination: 152 Annotation: Subtype: Link Rect: 160, 585, 165, 594 Flags: Print AppearanceDictionary: true Destination: 152 Annotation: Subtype: Link Rect: 447, 609, 457, 618 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 489, 549, 499, 558 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 529, 501, 538, 510 Flags: Print AppearanceDictionary: true Destination: 173 Annotation: Subtype: Link Rect: 323, 489, 328, 498 Flags: Print AppearanceDictionary: true Destination: 173 Thumb: true Page: Label: 8 Annotations: Annotation: Subtype: Link Rect: 185, 297, 195, 306 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 277, 261, 287, 270 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 80, 237, 90, 246 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 159, 213, 169, 222 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 254, 213, 264, 222 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 91, 201, 101, 210 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 335, 261, 344, 270 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 502, 153, 512, 162 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 307, 141, 317, 150 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 322, 141, 332, 150 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 466, 141, 476, 150 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 481, 141, 491, 150 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 369, 93, 379, 102 Flags: Print AppearanceDictionary: true Destination: 173 Annotation: Subtype: Link Rect: 178, 415, 186, 423 Flags: Print AppearanceDictionary: true Destination: 390 Thumb: true Page: Label: 9 Annotations: Annotation: Subtype: Link Rect: 115, 477, 125, 487 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 204, 321, 214, 330 Flags: Print AppearanceDictionary: true Destination: 173 Annotation: Subtype: Link Rect: 226, 321, 236, 330 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 176, 189, 186, 198 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 195, 141, 205, 150 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 103, 105, 113, 114 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 159, 93, 169, 102 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 347, 681, 356, 690 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 453, 381, 463, 390 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 468, 381, 477, 390 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 374, 177, 378, 186 Flags: Print AppearanceDictionary: true Destination: 221 Thumb: true Page: Label: 10 Annotations: Annotation: Subtype: Link Rect: 131, 381, 141, 390 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 146, 381, 156, 390 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 162, 381, 172, 390 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 165, 285, 175, 294 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 99, 261, 109, 270 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 116, 261, 126, 270 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 263, 261, 268, 270 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 275, 261, 285, 270 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 144, 249, 154, 258 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 159, 249, 174, 258 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 59, 237, 74, 246 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 81, 237, 96, 246 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 112, 189, 127, 198 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 270, 129, 285, 138 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 415, 429, 419, 438 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 446, 237, 460, 246 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 307, 105, 322, 114 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 365, 81, 380, 90 Flags: Print AppearanceDictionary: true Destination: 395 Thumb: true Page: Label: 11 Annotations: Annotation: Subtype: Link Rect: 100, 657, 110, 666 Flags: Print AppearanceDictionary: true Destination: 253 Annotation: Subtype: Link Rect: 307, 669, 322, 678 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 258, 173, 269, 181 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 380, 143, 390, 151 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 506, 123, 517, 131 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 442, 113, 453, 121 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 403, 93, 410, 101 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 197, 63, 208, 71 Flags: Print AppearanceDictionary: true Destination: 395 Thumb: true Page: Label: 12 Annotations: Annotation: Subtype: Link Rect: 259, 681, 273, 690 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 59, 645, 74, 654 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 275, 633, 285, 642 Flags: Print AppearanceDictionary: true Destination: 253 Annotation: Subtype: Link Rect: 99, 501, 108, 510 Flags: Print AppearanceDictionary: true Destination: 253 Annotation: Subtype: Link Rect: 120, 501, 135, 510 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 135, 297, 150, 306 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 82, 69, 97, 78 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 319, 693, 329, 702 Flags: Print AppearanceDictionary: true Destination: 390 Annotation: Subtype: Link Rect: 323, 513, 338, 522 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 320, 357, 325, 366 Flags: Print AppearanceDictionary: true Destination: 98 Annotation: Subtype: Link Rect: 304, 165, 314, 174 Flags: Print AppearanceDictionary: true Destination: 253 Annotation: Subtype: Link Rect: 325, 165, 339, 174 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 324, 57, 333, 66 Flags: Print AppearanceDictionary: true Destination: 253 Annotation: Subtype: Link Rect: 343, 57, 352, 66 Flags: Print AppearanceDictionary: true Destination: 383 Thumb: true Page: Label: 13 Annotations: Annotation: Subtype: Link Rect: 78, 561, 86, 570 Flags: Print AppearanceDictionary: true Destination: 253 Annotation: Subtype: Link Rect: 208, 513, 223, 522 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 228, 513, 243, 522 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 94, 429, 109, 438 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 103, 321, 112, 330 Flags: Print AppearanceDictionary: true Destination: 334 Annotation: Subtype: Link Rect: 124, 321, 138, 330 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 253, 249, 257, 258 Flags: Print AppearanceDictionary: true Destination: 98 Annotation: Subtype: Link Rect: 157, 105, 171, 114 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 467, 693, 477, 702 Flags: Print AppearanceDictionary: true Destination: 334 Annotation: Subtype: Link Rect: 486, 693, 501, 702 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 412, 513, 421, 522 Flags: Print AppearanceDictionary: true Destination: 334 Annotation: Subtype: Link Rect: 431, 513, 446, 522 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 323, 465, 337, 474 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 473, 453, 487, 462 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 507, 393, 521, 402 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 499, 237, 514, 246 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 482, 189, 497, 198 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 450, 81, 465, 90 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 474, 81, 489, 90 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 307, 57, 322, 66 Flags: Print AppearanceDictionary: true Destination: 395 Thumb: true Page: Label: 14 Annotations: Annotation: Subtype: Link Rect: 227, 273, 238, 281 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 447, 253, 458, 261 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 419, 233, 430, 241 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 324, 213, 327, 221 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 357, 203, 368, 211 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 516, 183, 527, 191 Flags: Print AppearanceDictionary: true Destination: 395 Thumb: true Page: Label: 15 Annotations: Annotation: Subtype: Link Rect: 277, 597, 287, 606 Flags: Print AppearanceDictionary: true Destination: 334 Annotation: Subtype: Link Rect: 59, 585, 64, 594 Flags: Print AppearanceDictionary: true Destination: 383 Annotation: Subtype: Link Rect: 219, 441, 228, 450 Flags: Print AppearanceDictionary: true Destination: 334 Annotation: Subtype: Link Rect: 242, 441, 257, 450 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 158, 189, 173, 198 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 90, 153, 106, 162 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 191, 81, 206, 90 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 387, 621, 402, 630 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 365, 453, 373, 462 Flags: Print AppearanceDictionary: true Destination: 334 Annotation: Subtype: Link Rect: 386, 453, 401, 462 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 370, 249, 385, 258 Flags: Print AppearanceDictionary: true Destination: 395 Annotation: Subtype: Link Rect: 307, 69, 322, 78 Flags: Print AppearanceDictionary: true Destination: 395 Thumb: true Page: Label: 16 Thumb: true Page: Label: 17 Thumb: true Page: Label: 18 Annotations: Annotation: Subtype: Link Rect: 423, 339, 514, 347 Flags: Print AppearanceDictionary: true Thumb: true Page: Label: 19 Thumb: true Checksum: 8bc8238c Type: CRC32 Checksum: 037bda6b4d872007ca50edc58dea3e44 Type: MD5 Checksum: bff6431bd2ce04f4c8338aba89d692aa1e006091 Type: SHA-1 Checksum: 0f24cf41003d7d9cee17501e543bc2caacbda290c7e5766d444f0f14312d568c Type: SHA-256