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Title:          A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates
Subject:        Scientific Reports, doi:10.1038/s41598-018-32213-8
Keywords:       
Author:         L. Gifre-Renom
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     Item: 
      Title: A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates
      Children: 
       Item: 
        Title: Results
        Children: 
         Item: 
          Title: Characterization of the M-SAA3 production in L. lactis. 
         Item: 
          Title: M-SAA3 purification from the soluble fraction. 
         Item: 
          Title: Solubilization and purification of M-SAA3 aggregates. 
         Item: 
          Title: Activity of the solubilized and purified M-SAA3. 
         Item: 
          Title: IB solubilization of prone-to-aggregate proteins. 
       Item: 
        Title: Discussion
       Item: 
        Title: Conclusions
       Item: 
        Title: Methods
        Children: 
         Item: 
          Title: Bacterial strains and plasmids. 
         Item: 
          Title: M-SAA3 protein production. 
         Item: 
          Title: Protein determination. 
         Item: 
          Title: Production and purification of the M-SAA3 from the soluble fraction. 
         Item: 
          Title: Optimization of the purification process of the soluble M-SAA3. 
         Item: 
          Title: Solubilization of M-SAA3 and MMP-9 from protein aggregates. 
         Item: 
          Title: Purification of the solubilized M-SAA3 and MMP-9. 
         Item: 
          Title: Identification of the main contaminating proteins. 
         Item: 
          Title: Activity of the solubilized M-SAA3. 
         Item: 
          Title: Activity of the solubilized MMP-9. 
         Item: 
          Title: Statistical analysis. 
       Item: 
        Title: Acknowledgements
       Item: 
        Title: Figure 1 Productions of M-SAA3 in L.
       Item: 
        Title: Figure 2 Protein gels for the soluble M-SAA3 IMAC purifications at different conditions (1, 3, 5, 6 and 8 in Table 1).
       Item: 
        Title: Figure 3 Schematic protocol for the solubilization of the M-SAA3 IBs produced in L lactis.
       Item: 
        Title: Figure 4 IMAC purification of the solubilized aggregates produced in L.
       Item: 
        Title: Figure 5 CXCL8 gene expression by bovine epithelial cells from the mammary gland with solubilized and purified M-SAA3, obtained from L.
       Item: 
        Title: Figure 6 Zymography of MMP-9.
       Item: 
        Title: Table 1 Tested conditions in the process to optimize the purification of the soluble M-SAA3.
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PRISM recommends that the PRISM Aggregation Type Controlled Vocabulary be used to provide values for this element. 
Note: PRISM recommends against the use of the #other value currently allowed in this controlled vocabulary. In lieu of using #other please reach out to the PRISM group at info@prismstandard.org to request addition of your term to the Aggregation Type Controlled Vocabulary. 

</pdfaProperty:description>
                           <pdfaProperty:name>aggregationType</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Copyright </pdfaProperty:description>
                           <pdfaProperty:name>copyright</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>The Digital Object Identifier for the article.
The DOI may also be used as the dc:identifier. If used as a dc:identifier, the URI form should be captured, and the bare identifier should also be captured using prism:doi. If an alternate unique identifier is used as the required dc:identifier, then the DOI should be specified as a bare identifier within prism:doi only. 
If the URL associated with a DOI is to be specified, then prism:url may be used in conjunction with prism:doi in order to provide the service endpoint (i.e. the URL). 
</pdfaProperty:description>
                           <pdfaProperty:name>doi</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
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                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>ISSN for an electronic version of the issue in which the resource occurs. 
Permits publishers to include a second ISSN, identifying an electronic version of the issue in which the resource occurs (therefore e(lectronic)Issn. If used, prism:eIssn MUST contain the ISSN of the electronic version. See prism:issn. 
</pdfaProperty:description>
                           <pdfaProperty:name>issn</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Title of the magazine, or other publication, in which a resource was/will be published. 
Typically this will be used to provide the name of the magazine an article appeared in as metadata for the article, along with information such as the article title, the publisher, volume, number, and cover date. 

Note: Publication name can be used to differentiate between a print magazine and the online version if the names are different such as “magazine” and “magazine.com.” 
</pdfaProperty:description>
                           <pdfaProperty:name>publicationName</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
                        </rdf:li>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>This element provides the url for an article or unit of content. 
The attribute platform is optionally allowed for situations in which multiple URLs must be specified. PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. 
NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. In lieu of using #other please reach out to the PRISM group at prism-wg@yahoogroups.com to request addition of your term to the Platform Controlled Vocabulary. 
</pdfaProperty:description>
                           <pdfaProperty:name>url</pdfaProperty:name>
                           <pdfaProperty:valueType>Text</pdfaProperty:valueType>
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                  <pdfaSchema:namespaceURI>http://www.niso.org/schemas/jav/1.0/</pdfaSchema:namespaceURI>
                  <pdfaSchema:prefix>jav</pdfaSchema:prefix>
                  <pdfaSchema:schema>NISO</pdfaSchema:schema>
                  <pdfaSchema:property>
                     <rdf:Seq>
                        <rdf:li rdf:parseType="Resource">
                           <pdfaProperty:category>external</pdfaProperty:category>
                           <pdfaProperty:description>Values for Journal Article Version are one of the following:
AO = Author’s Original
SMUR = Submitted Manuscript Under Review
AM = Accepted Manuscript
P = Proof
VoR = Version of Record
CVoR = Corrected Version of Record
EVoR = Enhanced Version of Record</pdfaProperty:description>
                           <pdfaProperty:name>journal_article_version</pdfaProperty:name>
                           <pdfaProperty:valueType>Closed Choice of Text</pdfaProperty:valueType>
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