122ac6c10adb51ae5d190fa73c377d07 pmc_30518772.pdf d16af493c6a95a9d766fae8053930ca0441d440e pmc_30518772.pdf 71a020f10be90ffaeb824b236969aa787823dae60f29ade076bdc61a7d3a6ce0 pmc_30518772.pdf Title: Effects of near infrared focused laser on the fluorescence of labelled cell membrane Subject: Scientific Reports, doi:10.1038/s41598-018-36010-1 Keywords: Author: Remy Avila Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Thu Nov 22 08:33:25 2018 CET ModDate: Thu Nov 29 19:27:56 2018 CET Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 4769528 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZFJLBK+Corbel CID TrueType Identity-H yes yes yes 345 0 PQPPRE+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 346 0 YRCROU+Corbel-BoldItalic Type 1C WinAnsi yes yes yes 196 0 WYXXPT+Corbel2 Type 1C WinAnsi yes yes yes 38 0 OCVNDG+Corbel-Bold CID TrueType Identity-H yes yes yes 39 0 MinionPro-Regular Type 1C Custom yes no yes 40 0 FDDHOH+Corbel-Bold2 Type 1C WinAnsi yes yes yes 258 0 WYXXPT+Corbel2 Type 1C WinAnsi yes yes yes 259 0 ZFJLBK+Corbel CID TrueType Identity-H yes yes yes 379 0 MyriadPro-It Type 1C WinAnsi yes no yes 260 0 EuclidSymbol Type 1C Custom yes no yes 34 0 MinionPro-Bold Type 1C WinAnsi yes no yes 35 0 VGEMXS+STIXGeneral-Regular Type 1C Custom yes yes yes 36 0 MinionPro-BoldIt Type 1C WinAnsi yes no yes 37 0 ZFJLBK+Corbel CID TrueType Identity-H yes yes yes 247 0 EuclidSymbol-Italic Type 1C Custom yes no yes 76 0 MinionPro-It Type 1C Custom yes no yes 77 0 ZFJLBK+Corbel CID TrueType Identity-H yes yes yes 273 0 OQANDG+EuclidExtra CID TrueType Identity-H yes yes yes 197 0 OQANDG+EuclidExtra CID TrueType Identity-H yes yes yes 198 0 OQANDG+EuclidExtra CID TrueType Identity-H yes yes yes 199 0 EuclidSymbol-Bold Type 1C Custom yes no yes 200 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-01-19 05:25:12 CET RepresentationInformation: pmc_30518772.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2019-08-12 03:38:42 CEST Size: 4769528 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 445 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Effects of near infrared focused laser on the fluorescence of labelled cell membrane Children: Item: Title: Results Children: Item: Title: Observed phenomena. Item: Title: Control measurements. Item: Title: Importance of free dye molecules in medium. Item: Title: Characterization. Item: Title: Fluorescence recovery after photobleaching. Item: Title: Discussion Item: Title: Conclusion Item: Title: Methods Children: Item: Title: Reagents. Item: Title: Cell culture. Item: Title: Sample preparation. Item: Title: Optical Setup. Item: Title: Measurements. Children: Item: Title: Fluorescence Intensity measurements. Item: Title: NIR laser power measurements. Item: Title: Data analysis and fitting. Children: Item: Title: Log-normal probability density function. Item: Title: FRAP. Item: Title: Demonstration of insensitivity of quantum yields Φa and Φb to the NIR laser. Children: Item: Title: Measurements out of the cell. Item: Title: Measurements on the cell. Item: Title: Analysis Software. Item: Title: Acknowledgements Item: Title: Figure 1 Effect of a 810-nm continuous wave focused laser on fluorescence intensity. Item: Title: Figure 2 Statistical analysis of the fluorescence rise upon exposure to NIR laser. Item: Title: Figure 3 Fluorescence Recovery After Photobleaching (FRAP). Item: Title: Figure 4 Plasma membrane stained with the fluorescent dye FM4-64. Item: Title: Figure 5 Timescale of fluorescence increase (a) and decay (b) as a result of NIR laser irradiation. Item: Title: Figure 6 Optical layout of the experiments. 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