01bc702e3c4b1d48d076652283648763 ijms-20-00059.pdf 6aa98bacd397bc8b1f167ee43f7e21a908e0e596 ijms-20-00059.pdf 9626580b5b7198f6f66c0acb8b0e5f69bf4664a65146b67dec9e5e46351dcdc1 ijms-20-00059.pdf Title: We Are What We Eat: A Stoichiometric and Ecometabolomic Study of Caterpillars Feeding on Two Pine Subspecies of Pinus sylvestris Subject: Many studies have addressed several plant-insect interaction topics at nutritional, molecular, physiological, and evolutionary levels. However, it is still unknown how flexible the metabolism and the nutritional content of specialist insect herbivores feeding on different closely related plants can be. We performed elemental, stoichiometric, and metabolomics analyses on leaves of two coexisting Pinus sylvestris subspecies and on their main insect herbivore; the caterpillar of the processionary moth (Thaumetopoea pityocampa). Caterpillars feeding on different pine subspecies had distinct overall metabolome structure, accounting for over 10% of the total variability. Although plants and insects have very divergent metabolomes, caterpillars showed certain resemblance to their plant-host metabolome. In addition, few plant-related secondary metabolites were found accumulated in caterpillar tissues which could potentially be used for self-defense. Caterpillars feeding on N and P richer needles had lower N and P tissue concentration and higher C:N and C:P ratios, suggesting that nutrient transfer is not necessarily linear through trophic levels and other plant-metabolic factors could be interfering. This exploratory study showed that little chemical differences between plant food sources can impact the overall metabolome of specialist insect herbivores. Significant nutritional shifts in herbivore tissues could lead to larger changes of the trophic web structure. Keywords: plant-insect; metabolomics; stoichiometry; processionary moth; scots pine; secondary metabolites; herbivory Author: Albert Rivas-Ubach, Josep Peñuelas, José Antonio Hódar, Michal Oravec, Ljiljana Paša-Tolić, Otmar Urban and Jordi Sardans Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Mon Dec 24 05:55:18 2018 CET ModDate: Mon Dec 24 05:55:18 2018 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5264998 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- CEDWTK+URWPalladioL-Ital Type 1 Custom yes yes no 87 0 WZESTU+URWPalladioL-Bold Type 1 Custom yes yes no 88 0 BYLZCQ+URWPalladioL-BoldItal Type 1 Custom yes yes no 89 0 HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes no 90 0 FCUSIS+URWPalladioL-Roma Type 1 Custom yes yes no 91 0 UFWDLZ+VnURWPalladioL Type 1 Custom yes yes no 92 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 97 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 98 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 117 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 118 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 119 0 ZZJXSH+EURM10 Type 1 Builtin yes yes no 216 0 XRAKTS+CMSY10 Type 1 Builtin yes yes no 218 0 NFHGHI+PalatinoLinotype CID TrueType Identity-H yes yes yes 241 0 NFHGHJ+PalatinoLinotype TrueType WinAnsi yes yes no 242 0 NFHGPI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 243 0 NFHGPK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 244 0 NFHHKJ+TimesNewRoman CID TrueType Identity-H yes yes yes 245 0 NFHGHI+PalatinoLinotype CID TrueType Identity-H yes yes yes 279 0 NFHGHJ+PalatinoLinotype TrueType WinAnsi yes yes no 280 0 NFHGPI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 281 0 NFHGPK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 282 0 NFHHKJ+TimesNewRoman CID TrueType Identity-H yes yes yes 283 0 NFHGHJ+PalatinoLinotype TrueType WinAnsi yes yes no 321 0 NFHGPI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 322 0 NFHGPK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 323 0 NFHHKJ+TimesNewRoman CID TrueType Identity-H yes yes yes 324 0 NFHGHJ+PalatinoLinotype TrueType WinAnsi yes yes no 374 0 NFHGPI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 375 0 NFHGPK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 376 0 NFHHKJ+TimesNewRoman CID TrueType Identity-H yes yes yes 377 0 SYFPBV+CMMI10 Type 1 Builtin yes yes no 544 0 FERHKG+CMR10 Type 1 Builtin yes yes no 545 0 java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary at edu.harvard.hul.ois.jhove.module.PdfModule.readDocCatalogDict(PdfModule.java:1284) at edu.harvard.hul.ois.jhove.module.PdfModule.parse(PdfModule.java:525) at edu.harvard.hul.ois.jhove.JhoveBase.processFile(JhoveBase.java:825) at edu.harvard.hul.ois.jhove.JhoveBase.process(JhoveBase.java:614) at edu.harvard.hul.ois.jhove.JhoveBase.dispatch(JhoveBase.java:465) at Jhove.main(Jhove.java:296) Jhove (Rel. 1.6, 2011-01-04) Date: 2019-01-19 04:53:04 CET RepresentationInformation: ijms-20-00059.pdf ReportingModule: BYTESTREAM, Rel. 1.3 (2007-04-10) LastModified: 2019-01-18 14:37:30 CET Size: 5264998 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: a48dbdd0 Type: CRC32 Checksum: 01bc702e3c4b1d48d076652283648763 Type: MD5 Checksum: 6aa98bacd397bc8b1f167ee43f7e21a908e0e596 Type: SHA-1