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Title:          Rice Seeds as Biofactories of Rationally Designed and Cell-Penetrating Antifungal PAF Peptides
Subject:        PAFs are short cationic and tryptophan-rich synthetic peptides with cell-penetrating antifungal activity.
Keywords:       PAF, antifungal, pathogens, fungi, rice, seed, oil bodies, protein bodies
Author:         María Coca
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     Item: 
      Title: Rice Seeds as Biofactories of Rationally Designed and Cell-Penetrating Antifungal 
PAF Peptides
      Children: 
       Item: 
        Title: Introduction
       Item: 
        Title: Materials and Methods
        Children: 
         Item: 
          Title: Preparation of Plant Expression Vectors
         Item: 
          Title: Production of Transgenic Plants
         Item: 
          Title: Protein Extraction and 
Immunoblot Analysis
         Item: 
          Title: RT-PCR Analysis of Transgene Expression
         Item: 
          Title: In situ Immunodetection of PAFs in 
Whole Seeds
         Item: 
          Title: Fungal Infection Assays on Rice Seeds
         Item: 
          Title: OBs Isolation and PAF102 Purification
         Item: 
          Title: Antifungal Assays
       Item: 
        Title: Results
        Children: 
         Item: 
          Title: Generation and Characterization of Transgenic Rice Plants
         Item: 
          Title: PAF103 Does Not Accumulate in PBs of Rice Seeds
         Item: 
          Title: PAF102 Accumulates as Fusion to the Ole18 Protein in Rice Seeds
         Item: 
          Title: Ole18-PAF102 Accumulation Has 
No Negative Impact in Rice 
Plant Performance
         Item: 
          Title: Ole18-PAF102 Accumulation Does 
Not Protect Rice Seeds Against 
Fungal Infection
         Item: 
          Title: Biologically Active PAF102 Is Recovered From pOle18:Ole18-PAF102 Seeds
       Item: 
        Title: Discussion
       Item: 
        Title: Ethics Statement
       Item: 
        Title: Author Contributions
       Item: 
        Title: Funding
     Item: 
      Title: Acknowledgments
     Item: 
      Title: References
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   Type: MD5
  Checksum: 444b08cc4dc768caed643e1d1457e988bab48d17
   Type: SHA-1