c8d5d3ac57c372fd7769a5d5d9f25eb3 microorganisms_a2019v9p292.pdf 5c3bf42dc3a3fb7eef3d7218e81434892e5ee2d7 microorganisms_a2019v9p292.pdf 24474eb3d85774530e3d31834aac1ad69b34d10bb578cddfedba7ba9e32c2ecf microorganisms_a2019v9p292.pdf Title: A Coordinated Response at The Transcriptome and Interactome Level is Required to Ensure Uropathogenic Escherichia coli Survival during Bacteremia Subject: Localized infections or disruption of the skin barrier can enable the entry of bacteria into the bloodstream, possibly leading to acute inflammation and sepsis. There is currently no holistic view on how bacteria can survive and spread in the bloodstream. In this context, we combined transposon mutagenesis, gene-expression profiling and a protein interaction network analysis to examine how uropathogenic Escherichia coli can proliferate in blood. Our results indicate that, upon migration from the urea to serum, E. coli reacts to the osmolarity difference, triggering a transcriptomic response in order to express survival genes. The proteins codified by these genes are precisely organized at the interactome level and specifically target short linear motifs located in disordered regions of host proteins. Such a coordinated response helps to explain how bacteria can adapt to and survive environmental changes within the host. Overall, our results provide a general framework for the study of bacteremia and reveal new targets for potential study as novel antimicrobials. Keywords: Escherichia coli; urinary tract infection; bacteremia; antimicrobials Author: Natalia Sanchez de Groot and Marc Torrent Burgas Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Sun Aug 25 09:39:23 2019 CEST ModDate: Sun Aug 25 09:39:23 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2499330 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- GIRLXX+URWPalladioL-Ital Type 1 Custom yes yes no 89 0 RVCRFI+URWPalladioL-Bold Type 1 Custom yes yes no 90 0 SFUDNE+URWPalladioL-BoldItal Type 1 Custom yes yes no 91 0 RLZZGA+URWPalladioL-Roma Type 1 Custom yes yes no 92 0 FDPKDP+VnURWPalladioL Type 1 Custom yes yes no 93 0 KTGNWG+Rpxr Type 1 Builtin yes yes no 94 0 SIXRFS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 99 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CID TrueType Identity-H yes no yes 300 0 CIDFont+F5 CID TrueType Identity-H yes no yes 301 0 CIDFont+F1 CID TrueType Identity-H yes no yes 357 0 CIDFont+F2 CID TrueType Identity-H yes no yes 358 0 CIDFont+F3 CID TrueType Identity-H yes no yes 359 0 CIDFont+F5 CID TrueType Identity-H yes no yes 360 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 453 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-02-15 05:33:28 CET RepresentationInformation: microorganisms_a2019v9p292.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-01-13 11:23:15 CET Size: 2499330 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰いちぴちぢちびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰うふのっぴどはのちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぇづのはねづぜ〴ぜ〰ぅのひどっとねづのぴぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰いどびはひつづひぜ〴ぜ〰ぐひはばづのびどぴべぜ〴ぜ〰ちのつぜ〴ぜ〰ことはひぴぜ〴ぜ〰がどのづちひぜ〴ぜ〰きはぴどてぜ〴ぜ〰ぃちぬっふぬちぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎづぴぷはひにぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: いちぴちぢちびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰うふのっぴどはのちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぇづのはねづぜ〴ぜ〰ぅのひどっとねづのぴぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰いどびはひつづひぜ〴ぜ〰ぐひはばづのびどぴべぜ〴ぜ〰ちのつぜ〴ぜ〰ことはひぴぜ〴ぜ〰がどのづちひぜ〴ぜ〰きはぴどてぜ〴ぜ〰ぃちぬっふぬちぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎづぴぷはひにぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: うふのっぴどはのちぬぜ〴ぜ〰ちのつぜ〴ぜ〰ぇづのはねづぜ〴ぜ〰ぅのひどっとねづのぴぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰いどびはひつづひぜ〴ぜ〰ぐひはばづのびどぴべぜ〴ぜ〰ちのつぜ〴ぜ〰ことはひぴぜ〴ぜ〰がどのづちひぜ〴ぜ〰きはぴどてぜ〴ぜ〰ぃちぬっふぬちぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎづぴぷはひにぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: いどびはひつづひぜ〴ぜ〰ぐひはばづのびどぴべぜ〴ぜ〰ちのつぜ〴ぜ〰ことはひぴぜ〴ぜ〰がどのづちひぜ〴ぜ〰きはぴどてぜ〴ぜ〰ぃちぬっふぬちぴどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎづぴぷはひにぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎづぴぷはひにぜ〴ぜ〰ぐちひちねづぴづひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴ぜ〰ちのつぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぃとちひちっぴづひどびちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴ぜ〰どのぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰ぉのてづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰いどててづひづのぴどちぬぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぇづのはねづぜ〴ぜ〰はひでちのどびちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのぴづひちっぴはねづぜ〴ぜ〰ぃはののづっぴどぶどぴべぜ〴ぜ〰はてぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぃははひつどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰えはびぴ〭ぐちぴとはでづのぜ〴ぜ〰ぉのぴづひちっぴはねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ぃとちひちっぴづひどびちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴ぜ〰どのぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰ぉのてづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰いどててづひづのぴどちぬぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぇづのはねづぜ〴ぜ〰はひでちのどびちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのぴづひちっぴはねづぜ〴ぜ〰ぃはののづっぴどぶどぴべぜ〴ぜ〰はてぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぃははひつどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰えはびぴ〭ぐちぴとはでづのぜ〴ぜ〰ぉのぴづひちっぴはねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどててづひづのぴどちぬぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぇづのはねづぜ〴ぜ〰はひでちのどびちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのぴづひちっぴはねづぜ〴ぜ〰ぃはののづっぴどぶどぴべぜ〴ぜ〰はてぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぃははひつどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰えはびぴ〭ぐちぴとはでづのぜ〴ぜ〰ぉのぴづひちっぴはねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぇづのはねづぜ〴ぜ〰はひでちのどびちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃうご〰〷〳ぜ〴ぜ〰あちっぴづひづねどちぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのぴづひちっぴはねづぜ〴ぜ〰ぃはののづっぴどぶどぴべぜ〴ぜ〰はてぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぃははひつどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰えはびぴ〭ぐちぴとはでづのぜ〴ぜ〰ぉのぴづひちっぴはねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぉのぴづひちっぴはねづぜ〴ぜ〰ぃはののづっぴどぶどぴべぜ〴ぜ〰はてぜ〴ぜ〰うどぴのづびび〭げづぬちぴづつぜ〴ぜ〰ぇづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ぃははひつどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰えはびぴ〭ぐちぴとはでづのぜ〴ぜ〰ぉのぴづひちっぴはねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃははひつどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰えはびぴ〭ぐちぴとはでづのぜ〴ぜ〰ぉのぴづひちっぴはねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: VSHNAE+Rpcxr FontSubset: true FirstChar: 169 LastChar: 169 FontDescriptor: FontName: VSHNAE+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: GBLQZB+Rpxi FontSubset: true FirstChar: 11 LastChar: 11 FontDescriptor: FontName: GBLQZB+Rpxi Flags: Symbolic FontBBox: -162, -280, 856, 733 FontFile: true 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