efeda1376567182a2c1c26fd1acaae28 appsci_a2019v9p4611.pdf ce92eb67f89fa25c91556bb2a286e375dc182da8 appsci_a2019v9p4611.pdf 51ec91888dc23d910f6ead1d84c1cb2c2d780d5ed9354d884e73819bb530dcd9 appsci_a2019v9p4611.pdf Title: Microbial Ecology Evaluation of an Iberian Pig Processing Plant through Implementing SCH Sensors and the Influence of the Resident Microbiota on Listeria monocytogenes Subject: There is a whole community of microorganisms capable of surviving the cleaning and disinfection processes in the food industry. These persistent microorganisms can enhance or inhibit biofilm formation and the proliferation of foodborne pathogens. Cleaning and disinfection protocols will never reduce the contamination load to 0; however, it is crucial to know which resident species are present and the risk they represent to pathogens, such as Listeria monocytogenes, as they can be further used as a complementary control strategy. The aim of this study was to evaluate the resident surface microbiota in an Iberian pig processing plant after carrying out the cleaning and disinfection processes. To do so, surface sensors were implemented, sampled, and evaluated by culture plate count. Further, isolated microorganisms were identified through biochemical tests. The results show that the surfaces are dominated by Bacillus spp., Pseudomonas spp., different enterobacteria, Mannheimia haemolytica, Rhizobium radiobacter, Staphylococcus spp., Aeromonas spp., lactic acid bacteria, and yeasts and molds. Moreover, their probable relationship with the presence of L. monocytogenes in three areas of the plant is also explained. Further studies of the resident microbiota and their interaction with pathogens such as L. monocytogenes are required. New control strategies that promote the most advantageous profile of microorganisms in the resident microbiota could be a possible alternative for pathogen control in the food industry. To this end, the understanding of the resident microbiota on the surfaces of the food industry and its relation with pathogen presence is crucial. Keywords: food industry; surface sensors; resident microbiota; microbial ecology; Listeria monocytogenes; ecological interrelations Author: Anne-Sophie Hascoët, Carolina Ripolles-Avila, Alfons Eduard Guerrero-Navarro and José Juan Rodríguez-Jerez Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Wed Oct 30 09:45:25 2019 CET ModDate: Wed Oct 30 09:45:25 2019 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2693675 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UMFMZN+URWPalladioL-Ital Type 1 Custom yes yes no 82 0 JAFPVW+URWPalladioL-Bold Type 1 Custom yes yes no 83 0 FNTQRE+URWPalladioL-BoldItal Type 1 Custom yes yes no 84 0 YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 85 0 HJRPXX+URWPalladioL-Roma Type 1 Custom yes yes no 86 0 CIQLLF+VnURWPalladioL Type 1 Custom yes yes no 87 0 WOCYLK+Rpxr Type 1 Builtin yes yes no 88 0 CRVMKC+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 93 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 110 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 111 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 112 0 QLZXDY+Pxsy Type 1 Builtin yes yes no 140 0 YICMPF+Rpcxr Type 1 Builtin yes yes no 177 0 LFGKGH+PalatinoLinotype TrueType WinAnsi yes yes no 182 0 LFGKLH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 183 0 LFGKMJ+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 184 0 GBLQZB+Rpxi Type 1 Builtin yes yes no 258 0 LFGKGH+PalatinoLinotype TrueType WinAnsi yes yes no 266 0 LFGKLH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 267 0 LFGKMJ+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 268 0 LFGKGH+PalatinoLinotype TrueType WinAnsi yes yes no 300 0 LFGKLH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 301 0 LFGKMJ+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 302 0 LFGKGH+PalatinoLinotype TrueType WinAnsi yes yes no 351 0 LFGKLH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 352 0 LFGKMJ+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 353 0 LFGKGH+PalatinoLinotype TrueType WinAnsi yes yes no 368 0 LFGKLH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 369 0 LFGKMJ+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 370 0 RDUGED+VnURWPalladioL-Italic Type 1 Custom yes yes no 460 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-01-29 05:29:01 CET RepresentationInformation: appsci_a2019v9p4611.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-01-14 10:36:18 CET Size: 2693675 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 3 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰こぴふつべぜ〴ぜ〰ぁばばひはちっとぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰こふひてちっづぜ〴ぜ〰こづのびはひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰ぐひはっづつふひづぜ〴ぜ〰ちのつぜ〴ぜ〰げづっはぶづひべぜ〴ぜ〰はてぜ〴ぜ〰ぁつとづひづつぜ〴ぜ〰きどっひははひでちのどびねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰きづぴとはつはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: こぴふつべぜ〴ぜ〰ぁばばひはちっとぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰こふひてちっづぜ〴ぜ〰こづのびはひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰ぐひはっづつふひづぜ〴ぜ〰ちのつぜ〴ぜ〰げづっはぶづひべぜ〴ぜ〰はてぜ〴ぜ〰ぁつとづひづつぜ〴ぜ〰きどっひははひでちのどびねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰きづぴとはつはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こふひてちっづぜ〴ぜ〰こづのびはひびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰こちねばぬどのでぜ〴ぜ〰ぐひはっづつふひづぜ〴ぜ〰ちのつぜ〴ぜ〰げづっはぶづひべぜ〴ぜ〰はてぜ〴ぜ〰ぁつとづひづつぜ〴ぜ〰きどっひははひでちのどびねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰きづぴとはつはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こちねばぬどのでぜ〴ぜ〰ぐひはっづつふひづぜ〴ぜ〰ちのつぜ〴ぜ〰げづっはぶづひべぜ〴ぜ〰はてぜ〴ぜ〰ぁつとづひづつぜ〴ぜ〰きどっひははひでちのどびねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰きづぴとはつはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぉつづのぴどてどっちぴどはのぜ〴ぜ〰きづぴとはつはぬはでべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いちぴちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴ぜ〰ちのつぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぉつづのぴどてどづつぜ〴ぜ〰こばづっどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぅっはぬはでどっちぬぜ〴ぜ〰ぐひはてどぬづびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰ぁひづちび〺ぜ〴ぜ〰ぃぬふびぴづひぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰さのふびふちぬぜ〴ぜ〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きちののとづどねどちぜ〴ぜ〰とちづねはぬべぴどっちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのてぬふづのっづぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰きどっひはぢどはぴちぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぐひづびづのっづぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰きはのはっべぴはでづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ぉつづのぴどてどづつぜ〴ぜ〰こばづっどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぅっはぬはでどっちぬぜ〴ぜ〰ぐひはてどぬづびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰ぁひづちび〺ぜ〴ぜ〰ぃぬふびぴづひぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰さのふびふちぬぜ〴ぜ〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きちののとづどねどちぜ〴ぜ〰とちづねはぬべぴどっちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのてぬふづのっづぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰きどっひはぢどはぴちぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぐひづびづのっづぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰きはのはっべぴはでづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぅっはぬはでどっちぬぜ〴ぜ〰ぐひはてどぬづびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰ぁひづちび〺ぜ〴ぜ〰ぃぬふびぴづひぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰さのふびふちぬぜ〴ぜ〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きちののとづどねどちぜ〴ぜ〰とちづねはぬべぴどっちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのてぬふづのっづぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰きどっひはぢどはぴちぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぐひづびづのっづぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰きはのはっべぴはでづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: さのふびふちぬぜ〴ぜ〰ぉつづのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きちののとづどねどちぜ〴ぜ〰とちづねはぬべぴどっちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぉのてぬふづのっづぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰きどっひはぢどはぴちぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぐひづびづのっづぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰きはのはっべぴはでづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぉのてぬふづのっづぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰きどっひはぢどはぴちぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぐひづびづのっづぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰きはのはっべぴはでづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: YICMPF+Rpcxr FontSubset: true FirstChar: 151 LastChar: 174 FontDescriptor: FontName: YICMPF+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: UMFMZN+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: UMFMZN+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GBLQZB+Rpxi FontSubset: true FirstChar: 11 LastChar: 11 FontDescriptor: FontName: GBLQZB+Rpxi Flags: Symbolic FontBBox: -162, -280, 856, 733 FontFile: true Font: BaseFont: JAFPVW+URWPalladioL-Bold FontSubset: true FirstChar: 2 LastChar: 235 FontDescriptor: FontName: JAFPVW+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: FNTQRE+URWPalladioL-BoldItal FontSubset: true FirstChar: 46 LastChar: 121 FontDescriptor: FontName: FNTQRE+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: YSPQJI+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 189 FontDescriptor: FontName: YSPQJI+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HJRPXX+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: HJRPXX+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: CIQLLF+VnURWPalladioL FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: CIQLLF+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WOCYLK+Rpxr FontSubset: true FirstChar: 11 LastChar: 47 FontDescriptor: FontName: WOCYLK+Rpxr Flags: Symbolic FontBBox: -40, -283, 904, 728 FontFile: true Font: BaseFont: QLZXDY+Pxsy FontSubset: true FirstChar: 6 LastChar: 14 FontDescriptor: FontName: QLZXDY+Pxsy Flags: Symbolic FontBBox: -21, -994, 1292, 912 FontFile: true Font: BaseFont: RDUGED+VnURWPalladioL-Italic FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: RDUGED+VnURWPalladioL-Italic Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 75, 757, 216, 793 Annotation: Subtype: Link Rect: 474, 757, 519, 793 Annotation: Subtype: Link Rect: 293, 619, 304, 633 Annotation: Subtype: Link Rect: 193, 606, 204, 619 Annotation: Subtype: Link Rect: 466, 527, 518, 546 Annotation: Subtype: Link Rect: 315, 85, 322, 94 ActionDest: 426 Annotation: Subtype: Link Rect: 171, 38, 242, 50 Annotation: Subtype: Link Rect: 405, 38, 519, 50 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 334, 743, 341, 752 ActionDest: 426 Annotation: Subtype: Link Rect: 344, 743, 351, 752 ActionDest: 426 Annotation: Subtype: Link Rect: 461, 729, 468, 738 ActionDest: 426 Annotation: Subtype: Link Rect: 212, 675, 219, 684 ActionDest: 426 Annotation: Subtype: Link Rect: 287, 634, 294, 644 ActionDest: 426 Annotation: Subtype: Link Rect: 385, 621, 397, 630 ActionDest: 426 Annotation: Subtype: Link Rect: 503, 607, 515, 617 ActionDest: 426 Annotation: Subtype: Link Rect: 197, 553, 209, 562 ActionDest: 426 Annotation: Subtype: Link Rect: 117, 513, 129, 522 ActionDest: 426 Annotation: Subtype: Link Rect: 269, 486, 281, 495 ActionDest: 426 Annotation: Subtype: Link Rect: 363, 472, 375, 481 ActionDest: 426 Annotation: Subtype: Link Rect: 116, 405, 128, 414 ActionDest: 426 Annotation: Subtype: Link Rect: 231, 364, 238, 373 ActionDest: 426 Annotation: Subtype: Link Rect: 449, 283, 461, 292 ActionDest: 426 Annotation: Subtype: Link Rect: 443, 242, 455, 252 ActionDest: 426 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 226, 619, 238, 628 ActionDest: 426 Annotation: Subtype: Link Rect: 405, 535, 412, 547 ActionDest: 163 Annotation: Subtype: Link Rect: 284, 524, 296, 533 ActionDest: 426 Annotation: Subtype: Link Rect: 99, 486, 110, 495 ActionDest: 426 Annotation: Subtype: Link Rect: 111, 205, 118, 217 ActionDest: 174 Annotation: Subtype: Link Rect: 245, 181, 257, 190 ActionDest: 457 Annotation: Subtype: Link Rect: 168, 140, 180, 149 ActionDest: 457 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 433, 547, 444, 556 ActionDest: 457 Annotation: Subtype: Link Rect: 503, 399, 515, 408 ActionDest: 457 Annotation: Subtype: Link Rect: 407, 194, 419, 203 ActionDest: 457 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 496, 729, 508, 738 ActionDest: 457 Annotation: Subtype: Link Rect: 317, 659, 329, 668 ActionDest: 457 Annotation: Subtype: Link Rect: 387, 455, 394, 467 ActionDest: 234 Annotation: Subtype: Link Rect: 397, 455, 404, 467 ActionDest: 255 Annotation: Subtype: Link Rect: 143, 415, 150, 427 ActionDest: 234 Annotation: Subtype: Link Rect: 460, 404, 472, 413 ActionDest: 426 Annotation: Subtype: Link Rect: 281, 364, 293, 373 ActionDest: 457 Annotation: Subtype: Link Rect: 488, 296, 500, 305 ActionDest: 457 Annotation: Subtype: Link Rect: 503, 296, 515, 305 ActionDest: 457 Annotation: Subtype: Link Rect: 391, 269, 403, 278 ActionDest: 457 Annotation: Subtype: Link Rect: 302, 242, 313, 251 ActionDest: 457 Annotation: Subtype: Link Rect: 238, 199, 246, 211 ActionDest: 234 Annotation: Subtype: Link Rect: 361, 188, 373, 197 ActionDest: 457 Annotation: Subtype: Link Rect: 373, 188, 385, 197 ActionDest: 457 Annotation: Subtype: Link Rect: 101, 147, 113, 157 ActionDest: 426 Annotation: Subtype: Link Rect: 364, 120, 376, 130 ActionDest: 457 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 321, 182, 333, 191 ActionDest: 457 Annotation: Subtype: Link Rect: 367, 155, 379, 164 ActionDest: 457 Annotation: Subtype: Link Rect: 130, 125, 137, 137 ActionDest: 234 Annotation: Subtype: Link Rect: 332, 87, 344, 96 ActionDest: 457 Annotation: Subtype: Link Rect: 345, 87, 357, 96 ActionDest: 457 Annotation: Subtype: Link Rect: 357, 87, 369, 96 ActionDest: 457 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 267, 545, 274, 557 ActionDest: 255 Annotation: Subtype: Link Rect: 491, 548, 503, 557 ActionDest: 457 Annotation: Subtype: Link Rect: 503, 548, 515, 557 ActionDest: 484 Annotation: Subtype: Link Rect: 291, 521, 303, 530 ActionDest: 484 Annotation: Subtype: Link Rect: 303, 521, 315, 530 ActionDest: 484 Annotation: Subtype: Link Rect: 271, 421, 278, 433 ActionDest: 255 Annotation: Subtype: Link Rect: 200, 95, 207, 107 ActionDest: 163 Annotation: Subtype: Link Rect: 141, 71, 153, 80 ActionDest: 426 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 404, 740, 411, 752 ActionDest: 292 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 196, 729, 208, 738 ActionDest: 457 Annotation: Subtype: Link Rect: 365, 729, 377, 738 ActionDest: 457 Annotation: Subtype: Link Rect: 377, 729, 389, 738 ActionDest: 484 Annotation: Subtype: Link Rect: 404, 715, 416, 725 ActionDest: 484 Annotation: Subtype: Link Rect: 141, 675, 153, 684 ActionDest: 484 Annotation: Subtype: Link Rect: 279, 661, 291, 671 ActionDest: 484 Annotation: Subtype: Link Rect: 303, 634, 315, 644 ActionDest: 484 Annotation: Subtype: Link Rect: 303, 618, 310, 630 ActionDest: 292 Annotation: Subtype: Link Rect: 117, 594, 129, 603 ActionDest: 484 Annotation: Subtype: Link Rect: 508, 564, 515, 576 ActionDest: 344 Annotation: Subtype: Link Rect: 338, 553, 350, 562 ActionDest: 484 Annotation: Subtype: Link Rect: 258, 486, 270, 495 ActionDest: 457 Annotation: Subtype: Link Rect: 443, 472, 455, 481 ActionDest: 484 Annotation: Subtype: Link Rect: 335, 445, 347, 454 ActionDest: 484 Annotation: Subtype: Link Rect: 190, 418, 201, 427 ActionDest: 484 Annotation: Subtype: Link Rect: 455, 364, 467, 373 ActionDest: 484 Annotation: Subtype: Link Rect: 447, 294, 454, 306 ActionDest: 344 Annotation: Subtype: Link Rect: 294, 256, 306, 265 ActionDest: 484 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 503, 71, 515, 80 ActionDest: 457 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 439, 702, 451, 711 ActionDest: 457 Annotation: Subtype: Link Rect: 353, 648, 365, 657 ActionDest: 484 Annotation: Subtype: Link Rect: 342, 621, 354, 630 ActionDest: 484 Annotation: Subtype: Link Rect: 404, 607, 416, 617 ActionDest: 484 Annotation: Subtype: Link Rect: 487, 538, 499, 547 ActionDest: 426 Annotation: Subtype: Link Rect: 508, 508, 515, 520 ActionDest: 255 Annotation: Subtype: Link Rect: 278, 484, 290, 493 ActionDest: 457 Annotation: Subtype: Link Rect: 410, 483, 417, 493 ActionDest: 426 Annotation: Subtype: Link Rect: 304, 443, 315, 452 ActionDest: 457 Annotation: Subtype: Link Rect: 316, 443, 328, 452 ActionDest: 484 Annotation: Subtype: Link Rect: 491, 429, 503, 439 ActionDest: 484 Annotation: Subtype: Link Rect: 503, 429, 515, 439 ActionDest: 484 Annotation: Subtype: Link Rect: 374, 389, 386, 398 ActionDest: 457 Annotation: Subtype: Link Rect: 379, 348, 391, 358 ActionDest: 484 Annotation: Subtype: Link Rect: 392, 348, 404, 358 ActionDest: 484 Annotation: Subtype: Link Rect: 436, 281, 448, 290 ActionDest: 484 Annotation: Subtype: Link Rect: 449, 281, 461, 290 ActionDest: 484 Annotation: Subtype: Link Rect: 411, 267, 423, 277 ActionDest: 426 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 284, 544, 322, 555 Annotation: Subtype: Link Rect: 328, 544, 364, 555 Annotation: Subtype: Link Rect: 308, 505, 346, 516 Annotation: Subtype: Link Rect: 352, 505, 388, 516 Annotation: Subtype: Link Rect: 127, 468, 165, 478 Annotation: Subtype: Link Rect: 171, 468, 207, 478 Annotation: Subtype: Link Rect: 375, 441, 413, 452 Annotation: Subtype: Link Rect: 336, 416, 373, 426 Annotation: Subtype: Link Rect: 310, 389, 348, 400 Annotation: Subtype: Link Rect: 378, 363, 415, 374 Annotation: Subtype: Link Rect: 303, 325, 341, 336 Annotation: Subtype: Link Rect: 172, 300, 210, 310 Annotation: Subtype: Link Rect: 441, 273, 479, 284 Annotation: Subtype: Link Rect: 158, 235, 196, 245 Annotation: Subtype: Link Rect: 136, 196, 174, 206 Annotation: Subtype: Link Rect: 291, 157, 329, 168 Annotation: Subtype: Link Rect: 335, 157, 371, 168 Annotation: Subtype: Link Rect: 286, 119, 324, 129 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 141, 728, 178, 738 Annotation: Subtype: Link Rect: 150, 638, 187, 648 Annotation: Subtype: Link Rect: 232, 573, 269, 583 Annotation: Subtype: Link Rect: 398, 546, 435, 557 Annotation: Subtype: Link Rect: 100, 508, 137, 519 Annotation: Subtype: Link Rect: 375, 482, 413, 493 Annotation: Subtype: Link Rect: 361, 443, 399, 454 Annotation: Subtype: Link Rect: 252, 405, 289, 415 Annotation: Subtype: Link Rect: 295, 405, 331, 415 Annotation: Subtype: Link Rect: 322, 366, 359, 376 Annotation: Subtype: Link Rect: 366, 366, 402, 376 Annotation: Subtype: Link Rect: 172, 328, 210, 338 Annotation: Subtype: Link Rect: 336, 288, 374, 299 Annotation: Subtype: Link Rect: 380, 288, 416, 299 Annotation: Subtype: Link Rect: 405, 249, 443, 260 Annotation: Subtype: Link Rect: 449, 249, 485, 260 Annotation: Subtype: Link Rect: 335, 224, 372, 234 Annotation: Subtype: Link Rect: 222, 186, 259, 196 Annotation: Subtype: Link Rect: 476, 159, 514, 170 Annotation: Subtype: Link Rect: 426, 120, 464, 131 Annotation: Subtype: Link Rect: 145, 82, 183, 92 Annotation: Subtype: Link Rect: 189, 82, 225, 92 Page: Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 100, 715, 137, 725 Annotation: Subtype: Link Rect: 143, 715, 179, 725 Annotation: Subtype: Link Rect: 438, 688, 475, 699 Annotation: Subtype: Link Rect: 292, 663, 330, 673 Annotation: Subtype: Link Rect: 336, 663, 372, 673 Annotation: Subtype: Link Rect: 393, 650, 519, 661 Annotation: Subtype: Link Rect: 97, 637, 247, 648 Annotation: Subtype: Link Rect: 349, 624, 387, 635 Annotation: Subtype: Link Rect: 418, 611, 519, 622 Annotation: Subtype: Link Rect: 97, 599, 271, 609 Annotation: Subtype: Link Rect: 387, 585, 424, 596 Annotation: Subtype: Link Rect: 418, 572, 519, 583 Annotation: Subtype: Link Rect: 97, 560, 270, 570 Annotation: Subtype: Link Rect: 313, 521, 350, 531 Annotation: Subtype: Link Rect: 100, 470, 137, 480 Annotation: Subtype: Link Rect: 100, 366, 137, 376 Annotation: Subtype: Link Rect: 404, 340, 441, 351 Annotation: Subtype: Link Rect: 447, 340, 483, 351 Annotation: Subtype: Link Rect: 150, 315, 187, 325 Annotation: Subtype: Link Rect: 261, 276, 298, 286 Annotation: Subtype: Link Rect: 304, 276, 340, 286 Annotation: Subtype: Link Rect: 259, 237, 297, 247 Annotation: Subtype: Link Rect: 76, 195, 166, 226 Annotation: Subtype: Link Rect: 234, 190, 407, 201 Checksum: 01d34b22 Type: CRC32 Checksum: efeda1376567182a2c1c26fd1acaae28 Type: MD5 Checksum: ce92eb67f89fa25c91556bb2a286e375dc182da8 Type: SHA-1