88516c63a3d887e252728220c9ddadf0 microorganisms_a2019v7p655.pdf 64bd8ea47261c278bdc7e268610fc9d03e42148e microorganisms_a2019v7p655.pdf 1d05f045acdbf26f2ba01c3a11641d3791212948f9ee2724ff35f0c7644f0958 microorganisms_a2019v7p655.pdf Title: Quantitative and Compositional Study of Monospecies Biofilms of Spoilage Microorganisms in the Meat Industry and Their Interaction in the Development of Multispecies Biofilms Subject: Food spoilage is a serious problem in the food industry, since it leads to significant economic losses. One of its main causes is the cross-contamination of food products from industrial surfaces. Three spoilage bacterial species which are highly present in meat and the gastrointestinal tract of chickens were selected: Pseudomonas fragi, Leuconostoc gasicomitatum, and Lactobacillus reuteri. The dual aim was to determine their ability to form monospecies biofilms and to examine how they interact when they coexist together. To do so, mature monospecies biofilms were produced statically for seven days at a temperature of 30 C. L. gasicomitatum was also used to investigate the behavior of P. fragi and L. reuteri in the formation of multispecies biofilms. The structure and composition of the monospecies biofilms were evaluated by direct epifluorescence microscopy, and the multispecies biofilms were evaluated by plate counting. Both L. gasicomitatum and L. reuteri were able to form biofilms, with counts of approximately 7 Log CFU/cm2 and a defined structure. However, P. fragi obtained counts to the order of 4 Log CFU/cm2, which is significantly different from the previous species (P < 0.05), and it had no network of cell conglomerates. The content of the L. gasicomitatum and L. reuteri biofilm matrices were 70–80% protein, unlike P. fragi, which presented a higher polysaccharide content (P < 0.05). In the multispecies biofilms, the presence of P. fragi did not affect the growth of L. gasicomitatum, which remained at between 5.76 to 6.1 Log CFU/cm2. However, L. reuteri was able to displace L. gasicomitatum growth after 24 h of coexisting in a mixed biofilm, presenting differences in counts of approximately 2 Log CFU/cm2. The study of the biofilms constructed by food industry resident microbiota can help to understand the ecological relations that exist between species, characterize them, and propose strategies to eliminate them. The name of genes and species should be written in italic. Keywords: spoilage bacteria; biofilms; ecological interactions; multispecies; control; food contact surfaces Author: Carolina Ripolles-Avila, Nerea García-Hernández, Brayan H. Cervantes-Huamán, Tina Mazaheri and José Juan Rodríguez-Jerez Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Thu Dec 5 09:40:53 2019 CET ModDate: Thu Dec 5 09:40:53 2019 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1947036 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OMEMAO+URWPalladioL-Ital Type 1 Custom yes yes no 104 0 UHEBQS+URWPalladioL-Bold Type 1 Custom yes yes no 105 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 106 0 AVCOCV+URWPalladioL-Roma Type 1 Custom yes yes no 107 0 MWHIBA+VnURWPalladioL Type 1 Custom yes yes no 108 0 GGBCKI+Rpxr Type 1 Builtin yes yes no 109 0 FCYHNG+Pxsy Type 1 Builtin yes yes no 110 0 SIXRFS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 115 0 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no 398 0 BDKBIM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 399 0 BDKBJN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 400 0 BDKCIJ+TimesNewRoman CID TrueType Identity-H yes yes yes 401 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 533 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-01-17 05:29:03 CET RepresentationInformation: microorganisms_a2019v7p655.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-01-16 11:10:55 CET Size: 1947036 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰こふひてちっづびぜ〴ぜ〰ぴはぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぬぜ〴ぜ〰こぴひちどのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Children: Item: Title: こふひてちっづびぜ〴ぜ〰ぴはぜ〴ぜ〰ごづびぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰あちっぴづひどちぬぜ〴ぜ〰こぴひちどのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Item: Title: あちっぴづひどちぬぜ〴ぜ〰こぴひちどのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Item: Title: きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Children: Item: Title: ぉのはっふぬふねぜ〴ぜ〰ぐひづばちひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸲ Item: Title: あどはてどぬねぜ〴ぜ〰うはひねちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸳ Item: Title: ぅぶちぬふちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぃづぬぬぜ〴ぜ〰ざどちぢどぬどぴべぜ〴ぜ〰ちのつぜ〴ぜ〰きちぴひどへぜ〴ぜ〰ぃはねばはびどぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰あどはてどぬねびぜ〴ぜ〰ぢべぜ〴ぜ〰いどひづっぴぜ〴ぜ〰ぅばどてぬふはひづびっづのぴぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰いぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰が〮ぜ〴ぜ〰でちびどっはねどぴちぴふねぜ〴ぜ〰ぐひづどねばぬちのぴちぴどはのぜ〴ぜ〰はのぜ〴ぜ〰こぴちどのぬづびびぜ〴ぜ〰こぴづづぬぜ〴ぜ〰こふひてちっづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぉぴびぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はのぜ〴ぜ〰こふぢびづぱふづのぴぜ〴ぜ〰ぃはぬはのどぺちぴどはのぜ〴ぜ〰ぢべぜ〴ぜ〰ぐ〮ぜ〴ぜ〰てひちでどぜ〴ぜ〰ちのつぜ〴ぜ〰が〮ぜ〴ぜ〰ひづふぴづひどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸴⸱ Item: Title: が〮ぜ〴ぜ〰でちびどっはねどぴちぴふねぜ〴ぜ〰ぐひづどねばぬちのぴちぴどはのぜ〴ぜ〰はのぜ〴ぜ〰こぴちどのぬづびびぜ〴ぜ〰こぴづづぬぜ〴ぜ〰こふひてちっづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぉぴびぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はのぜ〴ぜ〰こふぢびづぱふづのぴぜ〴ぜ〰ぃはぬはのどぺちぴどはのぜ〴ぜ〰ぢべぜ〴ぜ〰ぐ〮ぜ〴ぜ〰てひちでどぜ〴ぜ〰ちのつぜ〴ぜ〰が〮ぜ〴ぜ〰ひづふぴづひどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸴⸱ Children: Item: Title: ぉのはっふぬふねぜ〴ぜ〰ぐひづばちひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸴⸲ Item: Title: が〮ぜ〴ぜ〰でちびどっはねどぴちぴふねぜ〴ぜ〰ぐひづどねばぬちのぴちぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰こふぢびづぱふづのぴぜ〴ぜ〰ぃはぬはのどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸴⸳ Item: Title: きふぬぴどびばづっどづびぜ〴ぜ〰あどはてどぬねぜ〴ぜ〰ぅぶちぬふちぴどはのぜ〴ぜ〰ぢべぜ〴ぜ〰ぐぬちぴづぜ〴ぜ〰ぃはふのぴぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴ぜ〰ちのつぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぅぶちぬふちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰うはひねちぴどはのぜ〴ぜ〰ぃちばちっどぴべぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰きちぴひどへぜ〴ぜ〰ぃはねばはびどぴどはのぜ〴ぜ〰どのぜ〴ぜ〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづどねばぬちのぴちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰でちびどっはねどぴちぴふねぜ〴ぜ〰ちのつぜ〴ぜ〰ぉのてぬふづのっづぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰はてぜ〴ぜ〰ぐ〮ぜ〴ぜ〰てひちでどぜ〴ぜ〰ちのつぜ〴ぜ〰が〮ぜ〴ぜ〰ひづふぴづひどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ぅぶちぬふちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰うはひねちぴどはのぜ〴ぜ〰ぃちばちっどぴべぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰きちぴひどへぜ〴ぜ〰ぃはねばはびどぴどはのぜ〴ぜ〰どのぜ〴ぜ〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづどねばぬちのぴちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰でちびどっはねどぴちぴふねぜ〴ぜ〰ちのつぜ〴ぜ〰ぉのてぬふづのっづぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰はてぜ〴ぜ〰ぐ〮ぜ〴ぜ〰てひちでどぜ〴ぜ〰ちのつぜ〴ぜ〰が〮ぜ〴ぜ〰ひづふぴづひどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: きちぴひどへぜ〴ぜ〰ぃはねばはびどぴどはのぜ〴ぜ〰どのぜ〴ぜ〰きはのはびばづっどづびぜ〴ぜ〰あどはてどぬねびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづどねばぬちのぴちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰でちびどっはねどぴちぴふねぜ〴ぜ〰ちのつぜ〴ぜ〰ぉのてぬふづのっづぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰はてぜ〴ぜ〰ぐ〮ぜ〴ぜ〰てひちでどぜ〴ぜ〰ちのつぜ〴ぜ〰が〮ぜ〴ぜ〰ひづふぴづひどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぐひづどねばぬちのぴちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰が〮ぜ〴ぜ〰でちびどっはねどぴちぴふねぜ〴ぜ〰ちのつぜ〴ぜ〰ぉのてぬふづのっづぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰ぇひはぷぴとぜ〴ぜ〰はてぜ〴ぜ〰ぐ〮ぜ〴ぜ〰てひちでどぜ〴ぜ〰ちのつぜ〴ぜ〰が〮ぜ〴ぜ〰ひづふぴづひどぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: VSHNAE+Rpcxr FontSubset: true FirstChar: 169 LastChar: 169 FontDescriptor: FontName: VSHNAE+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: JNLIIF+Rpxi FontSubset: true FirstChar: 11 LastChar: 47 FontDescriptor: FontName: JNLIIF+Rpxi Flags: Symbolic FontBBox: -162, -280, 856, 733 FontFile: true Font: BaseFont: OMEMAO+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 151 FontDescriptor: FontName: OMEMAO+URWPalladioL-Ital Flags: 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