7afff344085930b2ee806ceb887818e8 pdf_222918.pdf 2cf84a4f72a94a6e455a49da48cb31fffdb51546 pdf_222918.pdf f5cf46cec0d5c4694bb060e0af8ffbc90b678bb945a372bd03af46b6c79f6c0b pdf_222918.pdf Title: Competitive USB-Powered Hand-Held Potentiostat for POC Applications: An HRP Detection Case Subject: Considerable efforts are made to develop Point-of-Care (POC) diagnostic tests. POC devices have the potential to match or surpass conventional systems regarding time, accuracy, and cost, and they are significantly easier to operate by or close to the patient. This strongly depends on the availability of miniaturized measurement equipment able to provide a fast and sensitive response. This paper presents a low-cost, portable, miniaturized USB-powered potentiostat for electrochemical analysis, which has been designed, fabricated, characterized, and tested against three forms of high-cost commercial equipment. The portable platform has a final size of 10.5 5.8 2.5 cm, a weight of 41 g, and an approximate manufacturing cost of $85 USD. It includes three main components: the power module which generates a stable voltage and a negative supply, the front-end module that comprises a dual-supply potentiostat, and the back-end module, composed of a microcontroller unit and a LabVIEW-based graphic user interface, granting plug-and-play and easy-to-use operation on any computer. The performance of this prototype was evaluated by detecting chronoamperometrically horseradish peroxidase (HRP), the enzymatic label most widely used in electrochemical biosensors. As will be shown, the miniaturized platform detected HRP at concentrations ranging from 0.01 ngmL-1 to 1 gmL-1, with results comparable to those obtained with the three commercial electrochemical systems. Keywords: horseradish peroxidase (HRP) chronoamperometry; electrochemical biosensor; reconfigurable potentiostat; portable; low-cost electronics; USB-powered Author: Yaiza Montes-Cebrián, Albert Álvarez-Carulla, Gisela Ruiz-Vega, Jordi Colomer-Farrarons, Manel Puig-Vidal, Eva Baldrich and Pere Ll. Miribel-Català Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Dec 6 11:00:25 2019 CET ModDate: Tue Feb 16 11:30:36 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2739139 bytes Optimized: yes PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- CMFVHJ+VnURWPalladioL Type 1 Custom yes yes no 522 0 DLBJBR+URWPalladioL-Ital Type 1 Custom yes yes no 526 0 TWXJKR+URWPalladioL-Bold Type 1 Custom yes yes no 529 0 WREYOJ+URWPalladioL-Roma Type 1 Custom yes yes no 532 0 SVVJCD+Rpxr Type 1 Builtin yes yes no 535 0 BZABPC+Pxsy Type 1 Builtin yes yes no 538 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 541 0 SWEGHG+VnURWPalladioL-Bold Type 1 Custom yes yes no 544 0 RVSEMT+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 548 0 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yes 278 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 329 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-04-29 02:36:20 CEST RepresentationInformation: pdf_222918.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-02-16 11:30:56 CET Size: 2739139 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Portable Potentiostat Children: Item: Title: Front-End Module (FEM): Signal Acquisition Module Description Item: Title: Back-End Module (BEM): Processing and Display Module Description Item: Title: Power Module (PM): Power Supply Module Description Item: Title: Electrochemical Measurements Children: Item: Title: Electrochemical HRP Detection Procedure Item: Title: Data Analysis Item: Title: Results and Discussion Children: Item: Title: Test and Calibration of the Electronic Platform Item: Title: System Verification by HRP Electrochemical Detection Item: Title: Conclusions Item: Title: References Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: SWEGHG+VnURWPalladioL-Bold FontSubset: true FirstChar: 129 LastChar: 161 FontDescriptor: FontName: SWEGHG+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TWXJKR+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: TWXJKR+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true 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Miribel-Català Considerable efforts are made to develop Point-of-Care (POC) diagnostic tests. POC devices have the potential to match or surpass conventional systems regarding time, accuracy, and cost, and they are significantly easier to operate by or close to the patient. This strongly depends on the availability of miniaturized measurement equipment able to provide a fast and sensitive response. This paper presents a low-cost, portable, miniaturized USB-powered potentiostat for electrochemical analysis, which has been designed, fabricated, characterized, and tested against three forms of high-cost commercial equipment. The portable platform has a final size of 10.5 5.8 2.5 cm, a weight of 41 g, and an approximate manufacturing cost of $85 USD. It includes three main components: the power module which generates a stable voltage and a negative supply, the front-end module that comprises a dual-supply potentiostat, and the back-end module, composed of a microcontroller unit and a LabVIEW-based graphic user interface, granting plug-and-play and easy-to-use operation on any computer. The performance of this prototype was evaluated by detecting chronoamperometrically horseradish peroxidase (HRP), the enzymatic label most widely used in electrochemical biosensors. As will be shown, the miniaturized platform detected HRP at concentrations ranging from 0.01 ngmL-1 to 1 gmL-1, with results comparable to those obtained with the three commercial electrochemical systems. Competitive USB-Powered Hand-Held Potentiostat for POC Applications: An HRP Detection Case 2019-12-06T18:00:25+08:00 LaTeX with hyperref package 2021-02-16T11:30:36+01:00 2021-02-16T11:30:36+01:00 horseradish peroxidase (HRP) chronoamperometry; electrochemical biosensor; reconfigurable potentiostat; portable; low-cost electronics; USB-powered pdfTeX-1.40.18 False This is pdfTeX, Version 3.14159265-2.6-1.40.18 (TeX Live 2017/W32TeX) kpathsea version 6.2.3 uuid:77c85422-f200-4e43-ac45-b1b4ff6d56be uuid:9f91a42b-7599-4ad8-b421-0055342809ba Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 75, 757, 194, 793 Annotation: Subtype: Link Rect: 474, 757, 519, 793 Annotation: Subtype: Link Rect: 189, 660, 200, 674 Annotation: Subtype: Link Rect: 314, 660, 325, 674 Annotation: Subtype: Link Rect: 284, 646, 295, 660 Annotation: Subtype: Link Rect: 368, 646, 379, 660 Annotation: Subtype: Link Rect: 508, 646, 519, 660 Annotation: Subtype: Link Rect: 466, 532, 518, 551 Annotation: 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