d480f389cc4cf793a644ff6332f7c009 SCR_a2019v9p13616.pdf fca6935e48088074b6b352928e10fc514fa728ca SCR_a2019v9p13616.pdf 6436a10ccce5c4b24dd04177278a1e4737a1f14849473603f1c19c3ca1a29c99 SCR_a2019v9p13616.pdf Title: Comparative analysis of the fecal microbiota from different species of domesticated and wild suids Subject: Scientific Reports, doi:10.1038/s41598-019-49897-1 Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Thu Sep 12 23:49:55 2019 CEST ModDate: Mon Sep 16 12:06:53 2019 CEST Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 3166978 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- NTEHND+Corbel-Bold CID TrueType Identity-H yes yes yes 135 0 DBGAXW+Corbel-BoldItalic Type 1C WinAnsi yes yes yes 133 0 MinionPro-Regular Type 1C Custom yes no yes 66 0 MDNQMP+Corbel-Bold2 Type 1C WinAnsi yes yes yes 67 0 LYSOWF+Corbel2 Type 1C WinAnsi yes yes yes 69 0 TMFWTR+Corbel CID TrueType Identity-H yes yes yes 70 0 MyriadPro-It Type 1C WinAnsi yes no yes 71 0 YPTCLH+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 500 0 LYSOWF+Corbel2 Type 1C WinAnsi yes yes yes 536 0 EuclidSymbol Type 1C Custom yes no yes 65 0 MinionPro-It Type 1C Custom yes no yes 68 0 MinionPro-Bold Type 1C WinAnsi yes no yes 90 0 STIXGeneral-Bold Type 1C Custom yes no yes 100 0 MinionPro-BoldIt Type 1C WinAnsi yes no yes 101 0 STIXGeneral-BoldItalic Type 1C Custom yes no yes 102 0 TLHHOR+STIXGeneral-Regular Type 1C Custom yes yes yes 134 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-01-27 02:21:43 CET RepresentationInformation: SCR_a2019v9p13616.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-01-26 08:52:30 CET Size: 3166978 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 1. GIT microbiota composition of domestic pigs (sus scrofa) obtained from Africa (AFR pig), from a conventional commercial farm (COM pig) and from SPF facilities (SPF pig). The mean relative abundance (%) of OTUs found in feces is pres:58' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 1. GIT microbiota composition of domestic pigs (sus scrofa) obtained from Africa (AFR pig), from a conventional commercial farm (COM pig) and from SPF facilities (SPF pig). The mean relative abundance (%) of OTUs found in feces is pre:60' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 1. GIT microbiota composition of domestic pigs (sus scrofa) obtained from Africa (AFR pig), from a conventional commercial farm (COM pig) and from SPF facilities (SPF pig). The mean relative abundance (%) of OTUs found in feces is pr:62' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 2. GIT microbiota composition of warthogs (Phacochoerus africanus) coming from Africa (AFR warthog) and from captive animals from Barcelona (Spain) zoo (SPA warthog). The mean relative abundance (%) of OTUs found in feces is presented.:69' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 2. GIT microbiota composition of warthogs (Phacochoerus africanus) coming from Africa (AFR warthog) and from captive animals from Barcelona (Spain) zoo (SPA warthog). The mean relative abundance (%) of OTUs found in feces is presented:70' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 3. Alpha diversity on rarefied fecal samples from pigs (AFR, COM and SPF) and warthogs (AFR and SPA). Alpha diversity was compared between groups by measuring the Shannon-Wiener’s metrics (A). Species richness was computed through Chao:73' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 3. Alpha diversity on rarefied fecal samples from pigs (AFR, COM and SPF) and warthogs (AFR and SPA). Alpha diversity was compared between groups by measuring the Shannon-Wiener’s metrics (A). Species richness was computed through Cha:74' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 3. Alpha diversity on rarefied fecal samples from pigs (AFR, COM and SPF) and warthogs (AFR and SPA). Alpha diversity was compared between groups by measuring the Shannon-Wiener’s metrics (A). Species richness was computed through Ch:75' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 4. Principal Coordinate Analysis plots (jackknifed) representing beta diversity on rarefied samples. Beta diversity of fecal samples from pigs (AFR, COM and SPF) and warthogs (AFR and SPA) was computed through weighted (A) and unweighted:78' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 4. Principal Coordinate Analysis plots (jackknifed) representing beta diversity on rarefied samples. Beta diversity of fecal samples from pigs (AFR, COM and SPF) and warthogs (AFR and SPA) was computed through weighted (A) and unweighte:79' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 5. Venn diagram showing the number of shared OTUs among the core communities of all groups of wild and domestic pigs.:81' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 5. Venn diagram showing the number of shared OTUs among the core communities of all groups of wild and domestic pigs.:82' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 5. Venn diagram showing the number of shared OTUs among the core communities of all groups of wild and domestic pigs.:83' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd: Table 2. Mean relative abundance (%) of the OTUs exclusively found in the core microbial composition of feces from AFSV resistant animals.:84' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 6. Principal component analysis illustrating distances between predicted sample pathway profiles. PC1 accounts for 70.4% and PC2 10.8% of the variation in the PICRUSt predicted pathways. Samples are colored by either the pig group they b:87' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 6. Principal component analysis illustrating distances between predicted sample pathway profiles. PC1 accounts for 70.4% and PC2 10.8% of the variation in the PICRUSt predicted pathways. Samples are colored by either the pig group they :88' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 7. Prediction of changed KEGG pathways using PICRUSt analysis. Selected pathways found to be significantly different among the different animal groups are depicted based on their relation to infection diseases (A), metabolic pathways (:90' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 7. Prediction of changed KEGG pathways using PICRUSt analysis. Selected pathways found to be significantly different among the different animal groups are depicted based on their relation to infection diseases (A), metabolic pathways :91' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 7. Prediction of changed KEGG pathways using PICRUSt analysis. Selected pathways found to be significantly different among the different animal groups are depicted based on their relation to infection diseases (A), metabolic pathways:92' cannot be found Offset: 2814300 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_49897.indd:Figure 4. Principal Coordinate Analysis plots (jackknifed) representing beta diversity on rarefied samples. Beta diversity of fecal samples from pigs (AFR, COM and SPF) and warthogs (AFR and SPA) was computed through weighted (A) and unweight:114' cannot be found Offset: 2814300 MIMEtype: application/pdf PDFMetadata: Objects: 584 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Comparative analysis of the fecal microbiota from different species of domesticated and wild suids Children: Item: Title: Results Children: Item: Title: The fecal bacterial community in SPF and indigenous pigs from Africa. Item: Title: The fecal bacterial community in warthogs (Phacochoerus africanus). Item: Title: Diversity analysis. Item: Title: Description of the ASF resistant and susceptible core microbiota. Item: Title: Functional prediction. Item: Title: Discussion Item: Title: Methods Children: Item: Title: Animals and sample collection. Item: Title: DNA extraction and 16S rRNA sequencing. Item: Title: Sequencing and taxonomic analysis. Item: Title: Acknowledgements Item: Title: Figure 1 GIT microbiota composition of domestic pigs (sus scrofa) obtained from Africa (AFR pig), from a conventional commercial farm (COM pig) and from SPF facilities (SPF pig). Item: Title: Figure 2 GIT microbiota composition of warthogs (Phacochoerus africanus) coming from Africa (AFR warthog) and from captive animals from Barcelona (Spain) zoo (SPA warthog). Item: Title: Figure 3 Alpha diversity on rarefied fecal samples from pigs (AFR, COM and SPF) and warthogs (AFR and SPA). Item: Title: Figure 4 Principal Coordinate Analysis plots (jackknifed) representing beta diversity on rarefied samples. Item: Title: Figure 5 Venn diagram showing the number of shared OTUs among the core communities of all groups of wild and domestic pigs. Item: Title: Figure 6 Principal component analysis illustrating distances between predicted sample pathway profiles. Item: Title: Figure 7 Prediction of changed KEGG pathways using PICRUSt analysis. Item: Title: Table 1 Relative abundance of OTUs found the fecal microbiota of the different animals analyzed throughout this study. Item: Title: Table 2 Mean relative abundance (%) of the OTUs exclusively found in the core microbial composition of feces from AFSV resistant animals. 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