0fdb6a1529d5b3ed0d20786d96f09d8a SR_a2019v9p6545.pdf 873671fafb95d4500cda7efa84cd62f9e63e9988 SR_a2019v9p6545.pdf fcc98ce1d7f27a432f3ac64d089261e257aafc393df5e3d9c01e878dcaff5ba4 SR_a2019v9p6545.pdf Title: Antimicrobial removal on piglets promotes health and higher bacterial diversity in the nasal microbiota Subject: Scientific Reports, doi:10.1038/s41598-019-43022-y Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Thu Apr 18 20:00:38 2019 CEST ModDate: Fri Apr 19 15:11:20 2019 CEST Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 2283749 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WFHXXA+Corbel-Bold CID TrueType Identity-H yes yes yes 34 0 MEJHLG+Corbel-BoldItalic Type 1C WinAnsi yes yes yes 308 0 MinionPro-Regular Type 1C Custom yes no yes 36 0 DWSIRS+Corbel-Bold2 Type 1C WinAnsi yes yes yes 37 0 JBJSME+Corbel2 Type 1C WinAnsi yes yes yes 40 0 HIVQVE+Corbel CID TrueType Identity-H yes yes yes 41 0 MyriadPro-It Type 1C WinAnsi yes no yes 42 0 HJUSQE+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 309 0 JBJSME+Corbel2 Type 1C WinAnsi yes yes yes 237 0 MinionPro-It Type 1C Custom yes no yes 35 0 MinionPro-Bold Type 1C WinAnsi yes no yes 38 0 EuclidSymbol Type 1C Custom yes no yes 39 0 JBJSME+Corbel2 Type 1C WinAnsi yes yes yes 230 0 MinionPro-BoldIt Type 1C WinAnsi yes no yes 111 0 MEMKIU+STIXGeneral-Regular Type 1C Custom yes yes yes 112 0 STIXGeneral-Bold Type 1C Custom yes no yes 153 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-02-13 02:34:09 CET RepresentationInformation: SR_a2019v9p6545.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-02-12 13:07:22 CET Size: 2283749 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 1. Nasal microbiota of piglets from farms MT and MC before (1) and after (2) antimicrobial treatment removal. Microbiota rarefaction curve generated using Shannon diversity estimator with samples from farms MT (A) and MC (B). Samples :32' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 1. Nasal microbiota of piglets from farms MT and MC before (1) and after (2) antimicrobial treatment removal. Microbiota rarefaction curve generated using Shannon diversity estimator with samples from farms MT (A) and MC (B). Samples:33' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 1. Nasal microbiota of piglets from farms MT and MC before (1) and after (2) antimicrobial treatment removal. Microbiota rarefaction curve generated using Shannon diversity estimator with samples from farms MT (A) and MC (B). Sample:34' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 1. Nasal microbiota of piglets from farms MT and MC before (1) and after (2) antimicrobial treatment removal. Microbiota rarefaction curve generated using Shannon diversity estimator with samples from farms MT (A) and MC (B). Sampl:35' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 2. Principal Component Plots (jackknifed) representing beta diversity on rarefied samples. Beta diversity of nasal samples of piglets was computed through unweighted UniFrac analysis for both farms, MT and MC, in two different sampling t:38' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 3. Stability of the nasal microbiota of piglets from farm MT through three different sampling time-points. (A) Alpha diversity using Shannon diversity index over rarefied samples is compared before perinatal antibiotic elimination (MT1:43' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 3. Stability of the nasal microbiota of piglets from farm MT through three different sampling time-points. (A) Alpha diversity using Shannon diversity index over rarefied samples is compared before perinatal antibiotic elimination (MT:44' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd:Figure 3. Stability of the nasal microbiota of piglets from farm MT through three different sampling time-points. (A) Alpha diversity using Shannon diversity index over rarefied samples is compared before perinatal antibiotic elimination (M:46' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd: 10. Looft, T. et al. Bacteria, phages and pigs: the effects of in-feed antibiotics on the microbiome at different gut locations. ISME J. 8, 1566–1576 (2014). :53' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd: 12. Janczyk, P. et al. Parenteral long-acting amoxicillin reduces intestinal bacterial community diversity in piglets even 5 weeks after the administration. ISME J. 1, 180–183 (2007). :56' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd: 11. Schokker, D. et al. Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. PloS One 9, e100040 (2014). :69' cannot be found Offset: 1914446 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_43022.indd: 6. Correa-Fiz, F., Fraile, L. & Aragon, V. Piglet nasal microbiota at weaning may influence the development of Glässer’s disease during the rearing period. BMC Genomics 17 (2016). :77' cannot be found Offset: 1914446 MIMEtype: application/pdf PDFMetadata: Objects: 381 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Antimicrobial removal on piglets promotes health and higher bacterial diversity in the nasal microbiota Children: Item: Title: Results Children: Item: Title: Diversity and species richness from the nasal microbiota. Item: Title: Stability of microbiota composition after antimicrobial elimination. Item: Title: Health and production parameters in the nursery phase. Item: Title: Discussion Item: Title: Material and Methods Children: Item: Title: Farms and sampling. Item: Title: DNA extraction and sequencing. Item: Title: Read filtering and data analysis. Item: Title: Acknowledgements Item: Title: Figure 1 Nasal microbiota of piglets from farms MT and MC before (1) and after (2) antimicrobial treatment removal. Item: Title: Figure 2 Principal Component Plots (jackknifed) representing beta diversity on rarefied samples. Item: Title: Figure 3 Stability of the nasal microbiota of piglets from farm MT through three different sampling time-points. Item: Title: Table 1 Relative abundance of OTUs before and after elimination of antibiotic treatment in two different farms (>1% relative abundance). Item: Title: Table 2 Relative abundance of OTUs at third sampling time in MT farm compared with the previous samplings. Item: Title: Table 3 Production data collected from the nursery phases of MT and MC farms in three consecutive years. 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in the nasal microbiota 10.1038/s41598-019-43022-y http://dx.doi.org/10.1038/s41598-019-43022-y journal Scientific Reports © 2019, The Author(s) 2045-2322 10.1038/s41598-019-43022-y Springer 2019-04-19T15:11:20+02:00 2019-04-18T23:30:38+05:30 2019-04-19T15:11:20+02:00 True iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) VoR uuid:67befe2d-ec10-4929-bc66-a10e1becc389 uuid:db2bd4ee-6bf8-48d9-a9f6-495e197cc867 default 1 converted uuid:3992e2f8-e754-4b12-9d0c-86773fe67f93 converted to PDF/A-2b pdfToolbox 2019-04-18T23:31:21+05:30 2 B http://ns.adobe.com/xap/1.0/mm/ xmpMM XMP Media Management Schema internal UUID based identifier for specific incarnation of a document InstanceID URI internal The common identifier for all versions and renditions of a document. OriginalDocumentID URI http://ns.adobe.com/pdf/1.3/ pdf Adobe PDF Schema internal A name object indicating whether the document has been modified to include trapping information Trapped Text http://ns.adobe.com/pdfx/1.3/ pdfx pdfx internal ID of PDF/X standard GTS_PDFXVersion Text internal Conformance level of PDF/X standard GTS_PDFXConformance Text internal Company creating the PDF Company Text internal Date when document was last modified SourceModified Text internal Mirrors crossmark:DOI doi Text http://www.aiim.org/pdfa/ns/id/ pdfaid PDF/A ID Schema internal Part of PDF/A standard part Integer internal Amendment of PDF/A standard amd Text internal Conformance level of PDF/A standard conformance Text http://prismstandard.org/namespaces/basic/2.0/ prism Prism external The aggregation type specifies the unit of aggregation for a content collection. Comment PRISM recommends that the PRISM Aggregation Type Controlled Vocabulary be used to provide values for this element. 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