85f09bc5bf1495b8514df891585482f2 materials_a2019v12n3168.pdf adab52fe68032152bad6bc47b3d907cd7e9c15a7 materials_a2019v12n3168.pdf 6d4e786938389100153dc5dd990466de5933abd9043c7e936a664cb460934845 materials_a2019v12n3168.pdf Title: Biofunctionalization with a TGF-1 Inhibitor Peptide in the Osseointegration of Synthetic Bone Grafts: An In Vivo Study in Beagle Dogs Subject: Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial—one untreated and another submitted to biofunctionalization with a TGF-1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial’s granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial’s granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant’s osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials. Keywords: osseointegration; biofunctionalization; histomorphometrical analysis; scanning electron microscopy; TGF-1; synthetic bone graft Author: Andrea Cirera, Maria Cristina Manzanares, Pablo Sevilla, Monica Ortiz-Hernandez, Pablo Galindo-Moreno and Javier Gil Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.19 CreationDate: Fri Sep 27 11:34:22 2019 CEST ModDate: Fri Sep 27 11:34:22 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 23 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 6087714 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FBNIBR+URWPalladioL-Ital Type 1 Custom yes yes no 76 0 AFPDEA+URWPalladioL-Bold Type 1 Custom yes yes no 77 0 ZYYJXI+EURB10 Type 1 Builtin yes yes no 78 0 AOIDSV+URWPalladioL-Roma Type 1 Custom yes yes no 79 0 GJQQCT+Rpxr Type 1 Builtin yes yes no 80 0 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