66609f2dc7a973738336f252ee2a3272 MOME_a2020v32p15.pdf 6340cac6676e64481070918ac00dc707c2f37534 MOME_a2020v32p15.pdf f7836492e7a2945c2fcc37928a56dd7547c92500560d4931bc6b9ae47bf58cd1 MOME_a2020v32p15.pdf Title: BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice Subject: Molecular Metabolism, 32 (2020) 15-26. doi:10.1016/j.molmet.2019.11.016 Author: Jenny M. 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referenced object 'bib24' cannot be found Offset: 1401540 MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 911 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Children: Item: Title: 1. Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: 2. Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Children: Item: Title: 2.1. Increased hepatic and serum BMP4 levels following AAV8 BMP4 injections, but not in peripheral tissues Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: 2.2. AAV8 BMP4 gene therapy in obese mice does not induce weight loss or browning of WAT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: 2.3. Reduced BMP signaling in subcutaneous adipose tissue but not in skeletal muscle or in liver Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: 2.4. Improved insulin sensitivity and glucose homeostasis in obese AAV8 BMP4 mice Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: 2.5. Tissue glucose uptake Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: 2.6. BMP4 also enhances hepatic insulin signaling and reduces hepatic gluconeogenic enzymes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: 3. Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: 4. Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: 5. Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Children: Item: Title: 5.1. Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: 5.2. Recombinant adeno-associated viral vectors Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: 5.3. GTT, ITT, and PTT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 5.4. Oxygen consumption and activity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 5.5. Glucose uptake Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Children: Item: Title: 5.5.1. In vivo protocol Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 5.5.2. Measurement of plasma and tissue 14C-2DG activity Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 5.5.3. Calculation of tissue-specific glucose clearance (Kg’) and utilization (Rg’) indices Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 5.6. In vivo insulin stimulation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 5.7. Serum chemistry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: 5.8. Liver triglyceride content Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: 5.9. Insulin stimulation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Children: Item: Title: 5.9.1. Isolated adipocytes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: 5.9.2. Human hepatocyte cell line (IHH) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: 5.10. Immunoprecipitation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Children: Item: Title: 5.10.1. Immunoprecipitation of BMP4 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Item: Title: 5.10.2. Immunoprecipitation and acetylation analysis of PGC1-a Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@553a3d88 Item: Title: 5.11. BMP reporter assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6 Item: Title: 5.12. Immunoblotting Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5891e32e Item: Title: 5.13. Measurement of islet size Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@cb0ed20 Item: Title: 5.14. Gene expression analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8e24743 Item: Title: 5.15. Statistical calculations and figures Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74a10858 Item: Title: Author contributions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@23fe1d71 Item: Title: Funding Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@28ac3dc3 Item: Title: Acknowledgements Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@32eebfca Item: Title: Conflict of interest Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4e718207 Item: Title: Appendix A. Supplementary data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1d371b2d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@543c6f6d Info: Title: BMP4 gene therapy enhances insulin sensitivity but not adipose tissue browning in obese mice Author: Jenny M. 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