A                    Peak: 11.7 nm
                                    Pdi: 0.553
                                    Peak: 7.5 nm
                                                                                Peak: 11.7 nm
                                                                                Pdi: 0.492
                                                                                Peak: 11.7 nm




                       Volume (%)
                                    Pdi: 0.46                                   Pdi: 0.512
                                    Peak: 6.5 nm                                Peak: 11.7 nm
                                    Pdi: 0.453                                  Pdi: 0.488
                                                              His                                      Arg
                                                                                                    Size (nm)
                                     Peak: 11.7 nm                              Peak: 11.7 nm
                                     Pdi: 0.541                                 Pdi: 0.491
                       Volume (%)

                                     Peak: 11.7 nm                              Peak: 11.7 nm
                                     Pdi: 0.492                                 Pdi: 0.518
                                     Peak: 11.7 nm                              Peak: 11.7 nm
                                     Pdi: 0.498                                 Pdi: 0.496
                                                             Trp                                        Tyr
                                                                                                    Size (nm)
                                    T22-GFP-H6       Molar Ratio 1:1             Molar Ratio 3:1
                                                                                           *                **             ns
               B                                                            C                      Metallic divalent
                                                                                                                  Non-metallic divalent
                                                                                NP
             T22-GFP-H6 +                      T22-GFP-H6
                                               Peak: 11.7 nm                    BB
             EDTA                                                                                                      Monovalent
             Peak: 6.5 nm                      Pdi: 0.492
Volume (%)




                                                                Size (nm)




             Pdi: 0.504




                                                        Size (nm)           GFP-H6 -EDTA +EDTA Ni2+ Cu2+ Zn2+ Ca2+ Cs+          K+
                                                                                                        T22-GFP-H6