B+Ni2+
                                                                                                                                             Ultrastructural morphometry
                                                                                                                                                                                            T22 NPs
                                                                                                                                                                           Heavy atom localization
                                                                                Figure 2b
A                                                   B                                                          C




                                                                                                                                     -EDTA
GFP-H6           T22-GFP-H6                         14




                                                                                                                    T22-GFP-H6
             a      b    c                                                           *              *
                                                    12   NP
                                                         BB




                                                                                                                                     +EDTA
                                                    10
                                Size (nm)


                                                    8
                                       Volume (%)



                                                                                                                                                                                   *
                                                    6




                                                                                                                   µg Ni / mg prot
                                                    4
    Supramolecular structures

                         BBs                        2


                                                    0
                                                              GFP-H6 T22-GFP-H6 BPs         +EDTA       +BSF
                                                                                     C
                                                                           H6




                                                                                            E1




                                                                                                        R1
                                                              H6




                                                                                  C.
                                                                         P-
                                                            P-



                                                                       GF
                                                          GF




                                                                                             T22-GFP-H6
                                                                     2-
                                                                   T2




                                                                                                                                                       B              -EDTA               +EDTA