A   B               C            N T22              GFP    H6            C
                               RRWCYRKCYKGYCYRKCRGGSSRSSSKGEELFTGVVPILVE
                               LDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                               PTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQER
    Ni2+                       TISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILG
                               HKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSGHH
                               HHHHGSGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQS
                               ALSKDPNEKRDHMVLLEFVTAAGITHGMDELY
             Ni2+
    Ni2+
                                     T22-GFP-H6(Loop)
           Ni2+                      Peak: 6.5 nm
                                     Pdi: 0.522




                        Volume (%)
                                                             Size (nm)