a CTX-GFP-H6 CTX-KRKRK-GFP-H6 CTX GFP H6 CTX GFP H6 MCMPCFTTDHQMARKCDDCCGGKGRGK MCMPCFTTDHQMARKCDDCCGGKGRGKCY CYGPQCLCRGGSSRSSSKGEELFTGVVPI GPQCLCRKRKRKGGSSRSSSKGEELFTGVV LVELDGDVNGHKFSVSGEGEGDATYGKLT PILVELDGDVNGHKFSVSGEGEGDATYGKLTL LKFICTTGKLPVPWPTLVTTLTYGVQCFSR KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPD YPDHMKRHDFFKSAMPEGYVQERTISFKD HMKRHDFFKSAMPEGYVQERTISFKDDGNY DGNYKTRAEVKFEGDTLVNRIELKGIDFKE KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGH DGNILGHKLEYNYNSHNVYITADKQKNGIK KLEYNYNSHNVYITADKQKNGIKANFKIRHNIE ANFKIRHNIEDGSVQLADHYQQNTPIGDGP DGSVQLADHYQQNTPIGDGPVLLPDNHYLST VLLPDNHYLSTQSALSKDPNEKRDHMVLL QSALSKDPNEKRDHMVLLEFVTAAGITHGMD EFVTAAGITHGMDELYKHHHHHH 31.842 KDa ELYKHHHHHH 32.583 KDa b C C+S C C+S c 25 20 Volume (%) Volume (%) 20 11.36 ± 0.1 nm 11.31 ± 0.1 nm 12.75 ± 0.4 nm 11.81 ± 0.1 nm 15 Pdi: 0.6 Pdi: 0.8 Pdi: 0.5 Pdi: 0.6 15 10 10 5 C C+S 5 C C+S 0 0 1 10 1 100 10 1 10 1100 10 Size (nm) d 4.93 7.44 10.13 5.29 7.66 9.82 + SDS 1% BSA 10 % Optipro 20 5.42 GFP-H6 3.89 7.80 11.78 4.16 7.50 10.23 15 Volume (%) 10 5 0 1 10 Size (nm)