a




                                                                                            Intracellular fluorescence (units)
Intracellular fluorescence (units)

                                                  HeLa                                                                                                  U87MG




                                                                C       CTX-KRKRK-GFP-H6   C
                                                   CTX-GFP-H6
                                                                C+S                        C+S


                                                       Protein concentration(µM)                                                                                                                              Protein concentration(µM)

b                                                                                                                                c


                                                                                                                                     Intracellular fluorescence (units)
                                                                                                                                                                                                       3.5
           Intracellular fluorescence (%)




                                            100                                                                                                                                               303.0




                                                                                                                                                                  Intracellular fluorescence (units)
                                            80                                                                                                                                                         2.5



                                            60                                                                                                                                                202.0
                                                                                                                                                                                                       1.5
                                            40
                                                                                                                                                                                              101.0
                                            20
                                                                                                                                                                                                       0.5


                                             0                                                                                                                                                         00.0
                                                           C          C+S         C        C+S                                                                                                                       -H6          AMD+      -H6            AMD+
                                                                                                                                                                                                                  GFP -     -H6 +        GFP -       -H6 +        AMD3100
                                                                                                                                                                                                              T22-      -GFP                      GFP
                                                                                                                                                                                                                     T22
                                                           CTX-GFP-H6           CTX-KRKRK-GFP-H6                                                                                                                    T22-GFP-H6                    GFP-H6