dcb7a0ab96ae7caddbcedec9a19e08fd 253294.pdf 1bc06aefc6e85a511e6dd5d0909efe1558d79bc0 253294.pdf 72c5b9cc713ebb80fec0220b4dfd1aab23c5929b168602005180d77db487790a 253294.pdf Title: Plasma microRNA Profiling Reveals Novel Biomarkers of Epicardial Adipose Tissue: A Multidetector Computed Tomography Study Subject: Epicardial adipose tissue (EAT) constitutes a novel parameter for cardiometabolic risk assessment and a target for therapy. Here, we evaluated for the first time the plasma microRNA (miRNA) profile as a source of biomarkers for epicardial fat volume (EFV). miRNAs were profiled in plasma samples from 180 patients whose EFV was quantified using multidetector computed tomography. In the screening study, 54 deregulated miRNAs were identified in patients with high EFV levels (highest tertile) compared with matched patients with low EFV levels (lowest tertile). After filtering, 12 miRNAs were selected for subsequent validation. In the validation study, miR-15b-3p, miR-22-3p, miR-148a-3p miR-148b-3p and miR-590-5p were directly associated with EFV, even after adjustment for confounding factors (p value < 0.05 for all models). The addition of miRNA combinations to a model based on clinical variables improved the discrimination (area under the receiver-operating-characteristic curve (AUC) from 0.721 to 0.787). miRNAs correctly reclassified a significant proportion of patients with an integrated discrimination improvement (IDI) index of 0.101 and a net reclassification improvement (NRI) index of 0.650. Decision tree models used miRNA combinations to improve their classification accuracy. These results were reproduced using two proposed clinical cutoffs for epicardial fat burden. Internal validation corroborated the robustness of the models. In conclusion, plasma miRNAs constitute novel biomarkers of epicardial fat burden. Keywords: biomarker; cardiometabolic disease; epicardial adipose tissue; epicardial fat; epicardial fat volume; microRNA Author: David de Gonzalo-Calvo, David Vilades, Pablo Martínez-Camblor, Àngela Vea, Andreu Ferrero-Gregori, Laura Nasarre, Olga Bornachea, Jesus Sanchez Vega, Rubén Leta, Núria Puig, Sonia Benítez, Jose Luis Sanchez-Quesada, Francesc Carreras and Vicenta Llorente-Cortés Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Sat Jun 1 10:25:35 2019 CEST ModDate: Sat Jun 1 10:25:35 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2166567 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QWNFHV+URWPalladioL-Ital Type 1 Custom yes yes no 93 0 XCFZWD+URWPalladioL-Bold Type 1 Custom yes yes no 94 0 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2166567 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 2 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: ぅへばづひどねづのぴちぬぜ〴ぜ〰こづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰こぴふつべぜ〴ぜ〰ぐはばふぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぃはひはのちひべぜ〴ぜ〰ぃはねばふぴづつぜ〴ぜ〰ごはねはでひちばとべぜ〴ぜ〰ぁのでどはでひちばとべぜ〴ぜ〰ぜ〵ぜ〰ぃぃごぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰あぬははつぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぅばどっちひつどちぬぜ〴ぜ〰ぁつどばはびづぜ〴ぜ〰ごどびびふづぜ〴ぜ〰ぜ〵ぜ〰ぅぁごぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰えどでと〭こづのびどぴどぶづぜ〴ぜ〰ぃ〭げづちっぴどぶづぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぜ〵ぜ〰ぃげぐぜ〵ㅜ〰ぜ〴ぜ〰ぃはのっづのぴひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きどっひはげぎぁぜ〴ぜ〰ぉびはぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: こぴふつべぜ〴ぜ〰ぐはばふぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぃはひはのちひべぜ〴ぜ〰ぃはねばふぴづつぜ〴ぜ〰ごはねはでひちばとべぜ〴ぜ〰ぁのでどはでひちばとべぜ〴ぜ〰ぜ〵ぜ〰ぃぃごぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰あぬははつぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぅばどっちひつどちぬぜ〴ぜ〰ぁつどばはびづぜ〴ぜ〰ごどびびふづぜ〴ぜ〰ぜ〵ぜ〰ぅぁごぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰えどでと〭こづのびどぴどぶづぜ〴ぜ〰ぃ〭げづちっぴどぶづぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぜ〵ぜ〰ぃげぐぜ〵ㅜ〰ぜ〴ぜ〰ぃはのっづのぴひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きどっひはげぎぁぜ〴ぜ〰ぉびはぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃはひはのちひべぜ〴ぜ〰ぃはねばふぴづつぜ〴ぜ〰ごはねはでひちばとべぜ〴ぜ〰ぁのでどはでひちばとべぜ〴ぜ〰ぜ〵ぜ〰ぃぃごぁぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰あぬははつぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぅばどっちひつどちぬぜ〴ぜ〰ぁつどばはびづぜ〴ぜ〰ごどびびふづぜ〴ぜ〰ぜ〵ぜ〰ぅぁごぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰えどでと〭こづのびどぴどぶづぜ〴ぜ〰ぃ〭げづちっぴどぶづぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぜ〵ぜ〰ぃげぐぜ〵ㅜ〰ぜ〴ぜ〰ぃはのっづのぴひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きどっひはげぎぁぜ〴ぜ〰ぉびはぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: あぬははつぜ〴ぜ〰ぃはぬぬづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぅばどっちひつどちぬぜ〴ぜ〰ぁつどばはびづぜ〴ぜ〰ごどびびふづぜ〴ぜ〰ぜ〵ぜ〰ぅぁごぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰えどでと〭こづのびどぴどぶづぜ〴ぜ〰ぃ〭げづちっぴどぶづぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぜ〵ぜ〰ぃげぐぜ〵ㅜ〰ぜ〴ぜ〰ぃはのっづのぴひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きどっひはげぎぁぜ〴ぜ〰ぉびはぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぅばどっちひつどちぬぜ〴ぜ〰ぁつどばはびづぜ〴ぜ〰ごどびびふづぜ〴ぜ〰ぜ〵ぜ〰ぅぁごぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰えどでと〭こづのびどぴどぶづぜ〴ぜ〰ぃ〭げづちっぴどぶづぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぜ〵ぜ〰ぃげぐぜ〵ㅜ〰ぜ〴ぜ〰ぃはのっづのぴひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きどっひはげぎぁぜ〴ぜ〰ぉびはぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: えどでと〭こづのびどぴどぶづぜ〴ぜ〰ぃ〭げづちっぴどぶづぜ〴ぜ〰ぐひはぴづどのぜ〴ぜ〰ぜ〵ぜ〰ぃげぐぜ〵ㅜ〰ぜ〴ぜ〰ぃはのっづのぴひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰きどっひはげぎぁぜ〴ぜ〰ぉびはぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: きどっひはげぎぁぜ〴ぜ〰ぉびはぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: けふちのぴどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰きどっひはげぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰こぴふつべぜ〴ぜ〰ぐはばふぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぐひはてどぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぐづひてはひねちのっづぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちびぜ〴ぜ〰あどはねちひにづひびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ざちぬどつちぴどはのぜ〴ぜ〰さびどのでぜ〴ぜ〰ぁぬぴづひのちぴどぶづぜ〴ぜ〰ぃふぴはててびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: こぴふつべぜ〴ぜ〰ぐはばふぬちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぐひはてどぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぐづひてはひねちのっづぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちびぜ〴ぜ〰あどはねちひにづひびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ざちぬどつちぴどはのぜ〴ぜ〰さびどのでぜ〴ぜ〰ぁぬぴづひのちぴどぶづぜ〴ぜ〰ぃふぴはててびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぐひはてどぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぐづひてはひねちのっづぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちびぜ〴ぜ〰あどはねちひにづひびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ざちぬどつちぴどはのぜ〴ぜ〰さびどのでぜ〴ぜ〰ぁぬぴづひのちぴどぶづぜ〴ぜ〰ぃふぴはててびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ぐづひてはひねちのっづぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちびぜ〴ぜ〰あどはねちひにづひびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ざちぬどつちぴどはのぜ〴ぜ〰さびどのでぜ〴ぜ〰ぁぬぴづひのちぴどぶづぜ〴ぜ〰ぃふぴはててびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぐづひてはひねちのっづぜ〴ぜ〰はてぜ〴ぜ〰ぐぬちびねちぜ〴ぜ〰きどっひはげぎぁびぜ〴ぜ〰ちびぜ〴ぜ〰あどはねちひにづひびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㔩‾㸊⡜㌷㙜㌷㝜〰ざちぬどつちぴどはのぜ〴ぜ〰さびどのでぜ〴ぜ〰ぁぬぴづひのちぴどぶづぜ〴ぜ〰ぃふぴはててびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ざちぬどつちぴどはのぜ〴ぜ〰さびどのでぜ〴ぜ〰ぁぬぴづひのちぴどぶづぜ〴ぜ〰ぃふぴはててびぜ〴ぜ〰はてぜ〴ぜ〰ぅばどっちひつどちぬぜ〴ぜ〰うちぴぜ〴ぜ〰ざはぬふねづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: ALNGKL+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 228 FontDescriptor: FontName: ALNGKL+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: 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