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Destination: 1 Item: Title: Follow-up of treated chronic CD patients in DNDi-CH-E1224-001 and MSF-DNDi PCR sampling optimization studies Destination: 1 Item: Title: DISCUSSION Destination: 1 Children: Item: Title: Impact of serial sampling strategies on qPCR sensitivity. Destination: 1 Item: Title: Distribution of DTUs and parasitic loads. Destination: 1 Item: Title: Dynamics of bloodstream parasite burden in chronic CD. Destination: 1 Item: Title: qPCR as surrogate marker of therapeutic failure in CD clinical trials. Destination: 1 Item: Title: MATERIALS AND METHODS Destination: 1 Children: Item: Title: Ethics statement. Destination: 1 Item: Title: Subjects and samples. Destination: 1 Item: Title: DNA extraction. Destination: 1 Item: Title: Quantitative real-time PCR procedure. Destination: 1 Item: Title: Genotyping of T. cruzi discrete typing units. Destination: 1 Item: Title: Statistical analysis. 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