5578d8cfdb599ba9dd5baf2a34626baf pmc_30809129.pdf 7454b3433a6144b211aa8b095923ea17ef5e3c30 pmc_30809129.pdf cae1a578ed4061e3f2f605e00c3c6a25eb7736d71e26477c2406e5fff23490f0 pmc_30809129.pdf Title: Cholesterol Depletion Regulates Axonal Growth and Enhances Central and Peripheral Nerve Regeneration Subject: Axonal growth during normal development and axonal regeneration rely on the action of many receptor signaling systems and complexes, most of them located in specialized raft membrane microdomains with a precise lipid composition. Keywords: regeneration, cholesterol, lipid rafts, axon growth, growth cone, filopodia Author: Jaume del Valle and Eduardo Soriano Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Sat Feb 9 12:38:55 2019 CET ModDate: Sat Feb 9 14:19:02 2019 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 893134 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PABIFR+MinionPro-Regular Type 1 Custom yes yes no 39 0 OGBLKS+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 40 0 ZVZYLV+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 41 0 QOGEDB+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 46 0 ZPYCCJ+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 47 0 GVVPEV+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 48 0 GDIYJU+MinionPro-It Type 1 Custom yes yes no 89 0 EWYLZG+MTGU Type 1 Builtin yes yes no 96 0 YTEWXK+MTSYN Type 1 Builtin yes yes no 97 0 SPKOBT+HelveticaNeueLTStd-Md Type 1 Custom yes yes no 104 0 PAARCL+RBLMI Type 1 Builtin yes yes no 196 0 USENBE+MinionPro-Bold Type 1 Custom yes yes no 225 0 WLTEQU+MTGUB Type 1 Builtin yes yes no 306 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:52:03 CEST RepresentationInformation: pmc_30809129.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-06 04:15:16 CEST Size: 893134 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 857 FreeObjects: 7 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Cholesterol Depletion Regulates Axonal Growth and Enhances Central and Peripheral Nerve Regeneration Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Reagents and Antibodies Item: Title: Primary Cultures Children: Item: Title: Hippocampus Item: Title: Cerebellum Item: Title: Dorsal Root Ganglion (DRG) Item: Title: Drug Treatments in vitro Children: Item: Title: Growth Cone and Filopodium Experiments Item: Title: Axon Extension and Branching Experiments in DRG Neurons Item: Title: Axotomy Experiments Item: Title: Animals and Surgical Procedures Item: Title: Electrophysiology Tests Item: Title: Locomotion Evaluation Item: Title: Mechanical Algesimetry Item: Title: Pinprick Test Item: Title: Histology, Immunohistochemistry and Immunocytochemistry Item: Title: Image Analysis Item: Title: Statistical Analysis Item: Title: Results Children: Item: Title: Cholesterol Depletion Increases Growth Cone Area and Number of Filopodia in Hippocampal and EGL Cerebellar Axons Item: Title: Cholesterol Depletion Affects Growth Cone Area, Number of Filopodia, and Neurite Extension in DRG Neurons Item: Title: Cholesterol Depletion Improves Axonal Regeneration After Axotomy in vitro and ex vivo Item: Title: Treatment With MβCD Has No Effect on Healthy Mice Item: Title: Treatment With MβCD Alters the Integrity of the Lipid Raft by Reducing the Amount of Membrane Cholesterol Item: Title: Cholesterol Depletion Induced by MβCD Increases GAP43 in Sensory Neurons After Axotomy Item: Title: Cholesterol Depletion Induced by MβCD Improves Muscle Reinnervation and Sensory Responses in Mice Item: Title: Discussion Item: Title: Author Contributions Item: Title: Funding Item: Title: Supplementary Material Item: Title: References Info: Title: Cholesterol Depletion Regulates Axonal Growth and Enhances Central and Peripheral Nerve Regeneration Author: Jaume del Valle and Eduardo Soriano Subject: Axonal growth during normal development and axonal regeneration rely on the action of many receptor signaling systems and complexes, most of them located in specialized raft membrane microdomains with a precise lipid composition. Keywords: regeneration, cholesterol, lipid rafts, axon growth, growth cone, filopodia Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Sat Feb 09 13:08:55 CET 2019 ModDate: Sat Feb 09 14:49:02 CET 2019 ID: 0x675d0954b54b58346f76c94be2116712, 0x45fa62651fee2e449a5352055319a01a Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: EWYLZG+MTGU FontSubset: true FirstChar: 98 LastChar: 109 FontDescriptor: FontName: EWYLZG+MTGU Flags: Symbolic FontBBox: 25, -213, 839, 683 FontFile: true Font: BaseFont: YTEWXK+MTSYN FontSubset: true FirstChar: 0 LastChar: 67 FontDescriptor: FontName: YTEWXK+MTSYN Flags: Symbolic FontBBox: 0, -954, 1043, 900 FontFile: true Font: BaseFont: USENBE+MinionPro-Bold FontSubset: true FirstChar: 3 LastChar: 150 FontDescriptor: FontName: USENBE+MinionPro-Bold Flags: Symbolic FontBBox: -318, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: PAARCL+RBLMI FontSubset: true FirstChar: 60 LastChar: 60 FontDescriptor: FontName: PAARCL+RBLMI Flags: Symbolic FontBBox: -11, -230, 987, 750 FontFile: true Font: BaseFont: PABIFR+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 244 FontDescriptor: FontName: PABIFR+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: OGBLKS+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 2 LastChar: 150 FontDescriptor: FontName: OGBLKS+HelveticaNeueLTStd-Bd Flags: Symbolic FontBBox: -166, -218, 1078, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: SPKOBT+HelveticaNeueLTStd-Md FontSubset: true FirstChar: 40 LastChar: 121 FontDescriptor: FontName: SPKOBT+HelveticaNeueLTStd-Md Flags: Symbolic FontBBox: -165, -221, 1066, 952 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: ZVZYLV+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 2 LastChar: 243 FontDescriptor: FontName: ZVZYLV+HelveticaNeueLTStd-Lt Flags: Symbolic FontBBox: -166, -214, 1050, 967 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: QOGEDB+HelveticaNeueLTStd-BdIt FontSubset: true FirstChar: 42 LastChar: 121 FontDescriptor: FontName: QOGEDB+HelveticaNeueLTStd-BdIt Flags: Symbolic FontBBox: -166, -218, 1129, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: ZPYCCJ+HelveticaNeueLTStd-LtIt FontSubset: true FirstChar: 17 LastChar: 253 FontDescriptor: FontName: ZPYCCJ+HelveticaNeueLTStd-LtIt Flags: Symbolic FontBBox: -165, -214, 1099, 951 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GVVPEV+HelveticaNeueLTStd-MdIt FontSubset: true FirstChar: 42 LastChar: 243 FontDescriptor: FontName: GVVPEV+HelveticaNeueLTStd-MdIt Flags: Symbolic FontBBox: -165, -218, 1125, 947 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WLTEQU+MTGUB FontSubset: true FirstChar: 98 LastChar: 98 FontDescriptor: FontName: WLTEQU+MTGUB Flags: Symbolic FontBBox: 0, -205, 879, 699 FontFile: true Font: BaseFont: GDIYJU+MinionPro-It FontSubset: true FirstChar: 40 LastChar: 243 FontDescriptor: FontName: GDIYJU+MinionPro-It Flags: Symbolic FontBBox: -201, -360, 1684, 919 FontFile: true EncodingDictionary: Differences: true XMP: regeneration, cholesterol, lipid rafts, axon growth, growth cone, filopodia pdfTeX-1.40.15 1.5 application/pdf fncel-13-00040.tex Cholesterol Depletion Regulates Axonal Growth and Enhances Central and Peripheral Nerve Regeneration Axonal growth during normal development and axonal regeneration rely on the action of many receptor signaling systems and complexes, most of them located in specialized raft membrane microdomains with a precise lipid composition. 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659 Annotation: Subtype: Link Rect: 312, 640, 390, 649 Annotation: Subtype: Link Rect: 477, 612, 551, 621 Annotation: Subtype: Link Rect: 312, 604, 350, 611 Annotation: Subtype: Link Rect: 404, 527, 430, 536 Annotation: Subtype: Link Rect: 431, 527, 463, 536 Annotation: Subtype: Link Rect: 463, 527, 498, 536 Annotation: Subtype: Link Rect: 498, 527, 522, 536 Annotation: Subtype: Link Rect: 522, 527, 549, 536 Annotation: Subtype: Link Rect: 43, 29, 146, 38 Annotation: Subtype: Link Rect: 149, 29, 209, 38 Annotation: Subtype: Link Rect: 434, 29, 551, 38 Checksum: ba7bd409 Type: CRC32 Checksum: 5578d8cfdb599ba9dd5baf2a34626baf Type: MD5 Checksum: 7454b3433a6144b211aa8b095923ea17ef5e3c30 Type: SHA-1