ed4b4022c745833935134b4f9c5fc97d pmc_30874584.pdf a22db70ffd18401da0a1af410a9f89bdab310cef pmc_30874584.pdf 977fdb2d45a4883e15fcbe1feec0833ff1620eb38b9c631173fca3d1ef658c73 pmc_30874584.pdf Title: Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization Subject: Scientific Reports, doi:10.1038/s41598-019-39571-x Keywords: Author: Josamp#x000E9; Luis Toramp#x000E1;n Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Thu Mar 7 07:48:50 2019 CET ModDate: Mon Mar 11 11:33:02 2019 CET Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 2990616 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LPMBLM+Corbel-Bold CID TrueType Identity-H yes yes yes 99 0 DQPMDC+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 518 0 MinionPro-Regular Type 1C Custom yes no yes 101 0 KAPMDS+Corbel-Bold2 Type 1C WinAnsi yes yes yes 102 0 EuclidSymbol Type 1C Custom yes no yes 103 0 RSLRKP+Corbel2 Type 1C WinAnsi yes yes yes 104 0 SHCWFY+Corbel CID TrueType Identity-H yes yes yes 105 0 MyriadPro-It Type 1C WinAnsi yes no yes 106 0 RSLRKP+Corbel2 Type 1C WinAnsi yes yes yes 451 0 MinionPro-It Type 1C Custom yes no yes 100 0 MinionPro-Bold Type 1C WinAnsi yes no yes 127 0 JNJYEQ+STIXGeneral-Regular Type 1C Custom yes yes yes 168 0 EuclidSymbol-Italic Type 1C Custom yes no yes 376 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:50:07 CEST RepresentationInformation: pmc_30874584.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-03-02 04:24:42 CET Size: 2990616 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 2. Martin-Puig, S., Wang, Z. & Chien, K. R. Lives of a heart cell: tracing the origins of cardiac progenitors. Cell Stem Cell 2, 320–331 (2008). :35' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 17. Moscoso, I. et al. Podocalyxin-like protein 1 is a relevant marker for human c-kit⁜琀⬠挀愀爀搀椀愀挀 猀琀攀洀 挀攀氀氀猀⸀⃾＀䫾＀⸀⃾＀吀椀猀猀甀攀 䔀渀柾＀⸀⃾＀刀攀最攀滾＀⸀⃾＀䴀攀擾＀⸀Ⰰ⃾￾＀栀琀琀瀀猀㨀⼀⼀搀漀椀⸀漀爀最⼀㄀　⸀㄀　　㈀⼀琀攀爀洀⸀㄀㜀㤀㔀⻾￾￾￾＀ ㈀　㄀㌀⻾＀㨀㔀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌹ㄮ㠳㤠㔴㐮㠸″㤷⸴㘸‵㌲⸶㈲崯䠯丯䅐㰼⽎‷ㄠ〠刾㸾㸊敮摯扪ਲ㤠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾＀￾＀㄀㠀⻾￾＀￾￾￾￾＀䰀愀甀搀攀渀Ⰰ 䰀⸀⃾＀攀琀 愀泾＀⸀ 䄀氀氀漀最攀渀椀挀椀琀礀 漀昀 栀甀洀愀渀 挀愀爀搀椀愀挀 猀琀攀洀⼀瀀爀漀最攀渀椀琀漀爀 挀攀氀氀猀 漀爀挀栀攀猀琀爀愀琀攀搀 戀礀 瀀爀漀最爀愀洀洀攀搀 搀攀愀琀栀 氀椀最愀渀搀 ㄀⸀⃾＀䌀椀爀挀 刀攀猀⻾＀⃾＀㄀㄀㋾＀Ⰰ 㐀㔀ㄠጀ㐀㘀㐀 ㈀　㄀㌀⻾＀㨀㔀㔩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌹㠮㠰㠠㔴㐮㠸‴〴⸴㌷‵㌲⸶㈲崯䠯丯䅐㰼⽎‷㈠〠刾㸾㸊敮摯扪ਲ਼〠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾￾＀￾＀㄀㠀⻾￾＀￾￾￾￾＀䰀愀甀搀攀渀Ⰰ 䰀⸀⃾＀攀琀 愀泾＀⸀ 䄀氀氀漀最攀渀椀挀椀琀礀 漀昀 栀甀洀愀渀 挀愀爀搀椀愀挀 猀琀攀洀⼀瀀爀漀最攀渀椀琀漀爀 挀攀氀氀猀 漀爀挀栀攀猀琀爀愀琀攀搀 戀礀 瀀爀漀最爀愀洀洀攀搀 搀攀愀琀栀 氀椀最愀渀搀 ㄀⸀⃾＀䌀椀爀挀 刀攀猀⻾＀⃾＀㄀㄀㋾＀Ⰰ 㐀㔀ㄠጀ㐀㘀㐀 ㈀　㄀㌀⻾＀㨀㔀㘩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔰㤮〹㐠㔳㐮㜶㌠㔱㐮㜱‵㈲⸶㈲崯䠯丯䅐㰼⽎‷㌠〠刾㸾㸊敮摯扪ਲ਼ㄠ〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾＀￾＀㄀㤀⻾￾＀￾￾￾￾＀䔀渀最氀椀猀栀Ⰰ 䬀⸀ 䴀攀挀栀愀渀椀猀洀猀 漀昀 洀攀猀攀渀挀栀礀洀愀氀 猀琀爀漀洀愀氀 挀攀氀氀 椀洀洀甀渀漀ⴀ洀漀搀甀氀愀琀椀漀渀⸀⃾＀䤀洀洀甀渀漀氀 䌀攀氀氀 䈀椀漀氀⻾＀⃾＀㤀ㇾ＀Ⰰ ㄀㤠ጀ㈀㘀 ㈀　㄀㌀⻾＀㨀㔀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔱㘮〴㌠㔳㐮㜶㌠㔲ㄮ㘵㤠㔲㈮㘲㉝⽈⽎⽁值㰯丠㜴‰⁒㸾㸾੥湤潢樊㌲‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd: 20. Makkar, R. et al. Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial. Lancet 9819, 895–904 (2012). :58' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 18. Lauden, L. et al. Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1. Circ Res. 112, 451–464 (2013). :55' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 18. Lauden, L. et al. Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1. Circ Res. 112, 451–464 (2013). :56' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 19. English, K. Mechanisms of mesenchymal stromal cell immuno-modulation. Immunol Cell Biol. 91, 19–26 (2013). :57' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 20. Makkar, R. et al. Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial. Lancet 9819, 895–904 (2012). :58' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 22. Ishigami, S. et al. Intracoronary autologous cardiac progenitor cell transfer in patients with hypoplastic left heart syndrome: the TICAP prospective phase 1 controlled trial. Circ Res. 116, 653–664 (2015). :59' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 23. Marbán, E. Breakthroughs in cell therapy for heart disease: focus on cardiosphere-derived cells. Mayo Clin. Proc. 89, 850–858 (2014). :60' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 24. Crisostomo, V., Casado, J. G., Baez-Diaz, C., Blazquez, R. & Sanchez-Margallo, F. M. Allogeneic cardiac stem cell administration for acute myocardial infarction. Expert Rev Cardiovasc Ther. 3, 285–299 (2015). :61' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 25. Sanz-Ruiz, R. et al. Rationale and Design of a Clinical Trial to Evaluate the Safety and Efficacy of Intracoronary Infusion of Allogeneic Human Cardiac Stem Cells in Patients With Acute Myocardial Infarction and Left Ventricular Dysfuncti:62' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 26. Fernández-Avilés, F. et al. Safety and Efficacy of Intracoronary Infusion of Allogeneic Human Cardiac Stem Cells in Patients with ST-segment Elevation Myocardial Infarction and Left Ventricular Dysfunction: A Multicenter Randomized, Double-B:63' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 17. Moscoso, I. et al. Podocalyxin-like protein 1 is a relevant marker for human c-kit⁜琀⬠挀愀爀搀椀愀挀 猀琀攀洀 挀攀氀氀猀⸀⃾＀䫾＀⸀⃾＀吀椀猀猀甀攀 䔀渀柾＀⸀⃾＀刀攀最攀滾＀⸀⃾＀䴀攀擾＀⸀Ⰰ⃾￾＀栀琀琀瀀猀㨀⼀⼀搀漀椀⸀漀爀最⼀㄀　⸀㄀　　㈀⼀琀攀爀洀⸀㄀㜀㤀㔀⻾￾￾￾＀ ㈀　㄀㌀⻾＀㨀㘀㐩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐷㤮㜲㔠㐵㐮㜶㌠㐸㔮㐰㔠㐴㈮㘲㉝⽈⽎⽁值㰯丠㠱‰⁒㸾㸾੥湤潢樊㌹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd: 18. Lauden, L. et al. Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1. Circ Res. 112, 451–464 (2013). :65' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 18. Lauden, L. et al. Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1. Circ Res. 112, 451–464 (2013). :65' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 17. Moscoso, I. et al. Podocalyxin-like protein 1 is a relevant marker for human c-kit⁜琀⬠挀愀爀搀椀愀挀 猀琀攀洀 挀攀氀氀猀⸀⃾＀䫾＀⸀⃾＀吀椀猀猀甀攀 䔀渀柾＀⸀⃾＀刀攀最攀滾＀⸀⃾＀䴀攀擾＀⸀Ⰰ⃾￾＀栀琀琀瀀猀㨀⼀⼀搀漀椀⸀漀爀最⼀㄀　⸀㄀　　㈀⼀琀攀爀洀⸀㄀㜀㤀㔀⻾￾￾￾＀ ㈀　㄀㌀⻾＀㨀㘀㘩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐲㠮㐠㌱㈮㜶㌠㐳㐮〵″〰⸶㈲崯䠯丯䅐㰼⽎‸㌠〠刾㸾㸊敮摯扪਴ㄠ〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾￾￾￾＀￾＀㄀㠀⻾￾＀￾￾￾￾＀䰀愀甀搀攀渀Ⰰ 䰀⸀⃾＀攀琀 愀泾＀⸀ 䄀氀氀漀最攀渀椀挀椀琀礀 漀昀 栀甀洀愀渀 挀愀爀搀椀愀挀 猀琀攀洀⼀瀀爀漀最攀渀椀琀漀爀 挀攀氀氀猀 漀爀挀栀攀猀琀爀愀琀攀搀 戀礀 瀀爀漀最爀愀洀洀攀搀 搀攀愀琀栀 氀椀最愀渀搀 ㄀⸀⃾＀䌀椀爀挀 刀攀猀⻾＀⃾＀㄀㄀㋾＀Ⰰ 㐀㔀ㄠጀ㐀㘀㐀 ㈀　㄀㌀⻾＀㨀㘀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐳㔮㐰ㄠ㌱㈮㜶㌠㐴ㄮ〵″〰⸶㈲崯䠯丯䅐㰼⽎‸㐠〠刾㸾㸊敮摯扪਴㈠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾￾＀￾＀㈀㔀⻾￾＀￾￾￾￾＀匀愀渀稀ⴀ刀甀椀稀Ⰰ 刀⸀⃾＀攀琀 愀泾＀⸀ 刀愀琀椀漀渀愀氀攀 愀渀搀 䐀攀猀椀最渀 漀昀 愀 䌀氀椀渀椀挀愀氀 吀爀椀愀氀 琀漀 䔀瘀愀氀甀愀琀攀 琀栀攀 匀愀昀攀琀礀 愀渀搀 䔀昀昀椀挀愀挀礀 漀昀 䤀渀琀爀愀挀漀爀漀渀愀爀礀 䤀渀昀甀猀椀漀渀 漀昀 䄀氀氀漀最攀渀攀椀挀 䠀甀洀愀渀 䌀愀爀搀椀愀挀 匀琀攀洀 䌀攀氀氀猀 椀渀 倀愀琀椀攀渀琀猀 圀椀琀栀 䄀挀甀琀攀 䴀礀漀挀愀爀搀椀愀氀 䤀渀昀愀爀挀琀椀漀渀 愀渀搀 䰀攀昀琀 嘀攀渀琀爀椀挀甀氀愀爀 䐀礀猀昀甀渀挀琀㨀㘀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔴㘮㤷′㠲⸷㘳‵㔲⸵㠶′㜰⸶㈲崯䠯丯䅐㰼⽎‸㔠〠刾㸾㸊敮摯扪਴㌠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾＀￾＀㈀㜀⻾￾＀￾￾￾￾＀刀椀琀挀栀椀攀Ⰰ 䴀⸀ 䔀⸀⃾＀攀琀 愀泾＀⸀ 氀椀洀洀愀 瀀漀眀攀爀猀 搀椀昀昀攀爀攀渀琀椀愀氀 攀砀瀀爀攀猀猀椀漀渀 愀渀愀氀礀猀攀猀 昀漀爀 刀一䄀ⴀ猀攀焀甀攀渀挀椀渀最 愀渀搀 洀椀挀爀漀愀爀爀愀礀 猀琀甀搀椀攀猀⸀⃾＀一甀挀氀攀椀挀 䄀挀椀搀猀 刀攀猀攀愀爀挀棾＀⃾＀㐀㏾＀Ⰰ 攀㐀㜀 ㈀　㄀㔀⻾＀㨀㘀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈰㠮㤷㌠㈲㈮㜶㌠㈱㐮㔶㘠㈱〮㘲㉝⽈⽎⽁值㰯丠㠶‰⁒㸾㸾੥湤潢樊㐴‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of three CPC i:70' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 18. Lauden, L. et al. Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1. Circ Res. 112, 451–464 (2013). :67' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 25. Sanz-Ruiz, R. et al. Rationale and Design of a Clinical Trial to Evaluate the Safety and Efficacy of Intracoronary Infusion of Allogeneic Human Cardiac Stem Cells in Patients With Acute Myocardial Infarction and Left Ventricular Dysfunct:68' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 27. Ritchie, M. E. et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Research 43, e47 (2015). :69' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of three CPC :71' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of three CPC:72' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of three CP:73' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of three C:74' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of three :75' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of three:76' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classified (8,8:78' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classified (8,:79' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classified (8:80' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classified (:81' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classified :82' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classified:83' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 28. Ziegler, A. N., Chidambaram, S., Forbes, B. E., Wood, T. L. & Levison, S. W. Insulin-like growth factor-II (IGF-II) and IGF-II analogs with enhanced insulin receptor-a binding affinity promote neural stem cell expansion. J. Biol. Chem. 2:84' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classifie:85' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸠㔀─㨀㠀㘩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐲㔮㘶㠠㈰㌮㜶㌠㐳㌮㤳㤠ㄹㄮ㘲㉝⽈⽎⽁值㰯丠ㄶ〠〠刾㸾㸊敮摯扪਱㐹‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸠㔀㨀㠀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔴㈮㘳㈠㈰㌮㜶㌠㔵ㄮ㔲㐠ㄹㄮ㘲㉝⽈⽎⽁值㰯丠ㄶㄠ〠刾㸾㸊敮摯扪਱㔰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸠㨀㠀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐶㌮㤰㘠ㄷ㌮㜶㌠㐷㈮ㄷ㜠ㄶㄮ㘲㉝⽈⽎⽁值㰯丠ㄶ㈠〠刾㸾㸊敮摯扪਱㔱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸀㨀㠀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈴ㄮ㠶㜠ㄵ㌮㠸′㔰⸰ㄴ‱㐱⸶㈲崯䠯丯䅐㰼⽎‱㘳‰⁒㸾㸾੥湤潢樊ㄵ㈠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾￾￾￾￾￾￾＀䘀椀最甀爀攀 ㏾￾＀⻾￾＠˾￾￾＀嘀愀氀椀搀愀琀椀漀渀 漀昀 瀀爀漀琀攀椀渀猀 椀搀攀渀琀椀昀椀攀搀 戀礀 挀漀洀瀀愀爀愀琀椀瘀攀 瀀爀漀琀攀漀洀椀挀猀 愀渀搀 猀礀猀琀攀洀 戀椀漀氀漀最礀 愀渀愀氀礀猀椀猀⸀ ＀懾＀⳾＀拾＀ 䌀倀䌀 甀瀀爀攀最甀氀愀琀攀搀 ＀懾＀ 愀渀搀 搀漀眀渀爀攀最甀氀愀琀攀搀 挀愀琀攀最漀爀椀攀猀 ＀拾＀⃾＀瘀現＀⸀ 䴀匀䌀⸀ 倀爀漀琀攀椀渀猀 爀攀瀀爀攀猀攀渀琀攀搀 戀礀 琀栀爀攀攀 瀀攀瀀琀椀搀攀猀 瀀攀爀 瀀爀漀琀攀椀渀 漀爀 氀攀猀猀 䘀刀䐠㨀㤀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌲㔮㜷ㄠ㤳⸸㠰㈠㌳㐮㠴㌠㠱⸶㈲㉝⽈⽎⽁值㰯丠ㄶ㐠〠刾㸾㸊敮摯扪਱㔳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classifi:91' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸠㔀㨀㠀㜩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔴㈮㘳㈠㈰㌮㜶㌠㔵ㄮ㔲㐠ㄹㄮ㘲㉝⽈⽎⽁值㰯丠ㄶㄠ〠刾㸾㸊敮摯扪਱㔰‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸠㨀㠀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐶㌮㤰㘠ㄷ㌮㜶㌠㐷㈮ㄷ㜠ㄶㄮ㘲㉝⽈⽎⽁值㰯丠ㄶ㈠〠刾㸾㸊敮摯扪਱㔱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸀㨀㠀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈴ㄮ㠶㜠ㄵ㌮㠸′㔰⸰ㄴ‱㐱⸶㈲崯䠯丯䅐㰼⽎‱㘳‰⁒㸾㸾੥湤潢樊ㄵ㈠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾￾￾￾￾￾￾＀䘀椀最甀爀攀 ㏾￾＀⻾￾＠˾￾￾＀嘀愀氀椀搀愀琀椀漀渀 漀昀 瀀爀漀琀攀椀渀猀 椀搀攀渀琀椀昀椀攀搀 戀礀 挀漀洀瀀愀爀愀琀椀瘀攀 瀀爀漀琀攀漀洀椀挀猀 愀渀搀 猀礀猀琀攀洀 戀椀漀氀漀最礀 愀渀愀氀礀猀椀猀⸀ ＀懾＀⳾＀拾＀ 䌀倀䌀 甀瀀爀攀最甀氀愀琀攀搀 ＀懾＀ 愀渀搀 搀漀眀渀爀攀最甀氀愀琀攀搀 挀愀琀攀最漀爀椀攀猀 ＀拾＀⃾＀瘀現＀⸀ 䴀匀䌀⸀ 倀爀漀琀攀椀渀猀 爀攀瀀爀攀猀攀渀琀攀搀 戀礀 琀栀爀攀攀 瀀攀瀀琀椀搀攀猀 瀀攀爀 瀀爀漀琀攀椀渀 漀爀 氀攀猀猀 䘀刀䐠㨀㤀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌲㔮㜷ㄠ㤳⸸㠰㈠㌳㐮㠴㌠㠱⸶㈲㉝⽈⽎⽁值㰯丠ㄶ㐠〠刾㸾㸊敮摯扪਱㔳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classifi:91' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸠㨀㠀㠩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㐶㌮㤰㘠ㄷ㌮㜶㌠㐷㈮ㄷ㜠ㄶㄮ㘲㉝⽈⽎⽁值㰯丠ㄶ㈠〠刾㸾㸊敮摯扪਱㔱‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸀㨀㠀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈴ㄮ㠶㜠ㄵ㌮㠸′㔰⸰ㄴ‱㐱⸶㈲崯䠯丯䅐㰼⽎‱㘳‰⁒㸾㸾੥湤潢樊ㄵ㈠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾￾￾￾￾￾￾＀䘀椀最甀爀攀 ㏾￾＀⻾￾＠˾￾￾＀嘀愀氀椀搀愀琀椀漀渀 漀昀 瀀爀漀琀攀椀渀猀 椀搀攀渀琀椀昀椀攀搀 戀礀 挀漀洀瀀愀爀愀琀椀瘀攀 瀀爀漀琀攀漀洀椀挀猀 愀渀搀 猀礀猀琀攀洀 戀椀漀氀漀最礀 愀渀愀氀礀猀椀猀⸀ ＀懾＀⳾＀拾＀ 䌀倀䌀 甀瀀爀攀最甀氀愀琀攀搀 ＀懾＀ 愀渀搀 搀漀眀渀爀攀最甀氀愀琀攀搀 挀愀琀攀最漀爀椀攀猀 ＀拾＀⃾＀瘀現＀⸀ 䴀匀䌀⸀ 倀爀漀琀攀椀渀猀 爀攀瀀爀攀猀攀渀琀攀搀 戀礀 琀栀爀攀攀 瀀攀瀀琀椀搀攀猀 瀀攀爀 瀀爀漀琀攀椀渀 漀爀 氀攀猀猀 䘀刀䐠㨀㤀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌲㔮㜷ㄠ㤳⸸㠰㈠㌳㐮㠴㌠㠱⸶㈲㉝⽈⽎⽁值㰯丠ㄶ㐠〠刾㸾㸊敮摯扪਱㔳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classifi:91' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㸀㨀㠀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈴ㄮ㠶㜠ㄵ㌮㠸′㔰⸰ㄴ‱㐱⸶㈲崯䠯丯䅐㰼⽎‱㘳‰⁒㸾㸾੥湤潢樊ㄵ㈠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㌀㤀㔀㜀㄀⸀椀渀搀搀㫾￾￾￾￾￾￾＀䘀椀最甀爀攀 ㏾￾＀⻾￾＠˾￾￾＀嘀愀氀椀搀愀琀椀漀渀 漀昀 瀀爀漀琀攀椀渀猀 椀搀攀渀琀椀昀椀攀搀 戀礀 挀漀洀瀀愀爀愀琀椀瘀攀 瀀爀漀琀攀漀洀椀挀猀 愀渀搀 猀礀猀琀攀洀 戀椀漀氀漀最礀 愀渀愀氀礀猀椀猀⸀ ＀懾＀⳾＀拾＀ 䌀倀䌀 甀瀀爀攀最甀氀愀琀攀搀 ＀懾＀ 愀渀搀 搀漀眀渀爀攀最甀氀愀琀攀搀 挀愀琀攀最漀爀椀攀猀 ＀拾＀⃾＀瘀現＀⸀ 䴀匀䌀⸀ 倀爀漀琀攀椀渀猀 爀攀瀀爀攀猀攀渀琀攀搀 戀礀 琀栀爀攀攀 瀀攀瀀琀椀搀攀猀 瀀攀爀 瀀爀漀琀攀椀渀 漀爀 氀攀猀猀 䘀刀䐠㨀㤀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌲㔮㜷ㄠ㤳⸸㠰㈠㌳㐮㠴㌠㠱⸶㈲㉝⽈⽎⽁值㰯丠ㄶ㐠〠刾㸾㸊敮摯扪਱㔳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classifi:91' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD⁜琀㨀㤀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㌲㔮㜷ㄠ㤳⸸㠰㈠㌳㐮㠴㌠㠱⸶㈲㉝⽈⽎⽁值㰯丠ㄶ㐠〠刾㸾㸊敮摯扪਱㔳‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_39571.indd:Figure 2. Distribution and classification of CPC proteome compared with MSC and HDF proteomes. (a) Distribution of CPC label-free proteomic results, using isolate hCPC3. A total of 9,645 proteins were identified by Uniprot and classifi:91' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 3. Validation of proteins identified by comparative proteomics and system biology analysis. (a,b) CPC upregulated (a) and downregulated categories (b) vs. MSC. Proteins represented by three peptides per protein or less (FRD:92' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of thre:96' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 1. RNAseq analysis of CPC compared with MSC and HDF. (a,b) mRNAseq experiments were carried out and analyzed using the Ilumina platform, with replicates and/or technical duplicates of all samples (see Methods). Analysis of thre:96' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 4. Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. (a,b) Label-free experiments comparing CPC with MSC and HDF; Venn diagram representation of differential upregula:102' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 4. Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. (a,b) Label-free experiments comparing CPC with MSC and HDF; Venn diagram representation of differential upregul:105' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 18. Lauden, L. et al. Allogenicity of human cardiac stem/progenitor cells orchestrated by programmed death ligand 1. Circ Res. 112, 451–464 (2013). :112' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 4. Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. (a,b) Label-free experiments comparing CPC with MSC and HDF; Venn diagram representation of differential upregu:116' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 4. Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. (a,b) Label-free experiments comparing CPC with MSC and HDF; Venn diagram representation of differential upreg:117' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 4. Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. (a,b) Label-free experiments comparing CPC with MSC and HDF; Venn diagram representation of differential upre:118' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 4. Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. (a,b) Label-free experiments comparing CPC with MSC and HDF; Venn diagram representation of differential up:120' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 4. Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. (a,b) Label-free experiments comparing CPC with MSC and HDF; Venn diagram representation of differential u:121' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 5. Validation of putative CPC membrane markers. (a) RT-qPCR of CACNG7, CDH5, FR11, GPR4 gene expression from independent CPC donors (CPC1–3; blue bars), two HDF (HDF and F3; red bars), and  two independent MSC (MSC19, MSC45; gre:124' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 5. Validation of putative CPC membrane markers. (a) RT-qPCR of CACNG7, CDH5, FR11, GPR4 gene expression from independent CPC donors (CPC1–3; blue bars), two HDF (HDF and F3; red bars), and  two independent MSC (MSC19, MSC45; gr:125' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 5. Validation of putative CPC membrane markers. (a) RT-qPCR of CACNG7, CDH5, FR11, GPR4 gene expression from independent CPC donors (CPC1–3; blue bars), two HDF (HDF and F3; red bars), and  two independent MSC (MSC19, MSC45; g:126' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 5. Validation of putative CPC membrane markers. (a) RT-qPCR of CACNG7, CDH5, FR11, GPR4 gene expression from independent CPC donors (CPC1–3; blue bars), two HDF (HDF and F3; red bars), and  two independent MSC (MSC19, MSC45; :127' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 5. Validation of putative CPC membrane markers. (a) RT-qPCR of CACNG7, CDH5, FR11, GPR4 gene expression from independent CPC donors (CPC1–3; blue bars), two HDF (HDF and F3; red bars), and  two independent MSC (MSC19, MSC45;:128' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 5. Validation of putative CPC membrane markers. (a) RT-qPCR of CACNG7, CDH5, FR11, GPR4 gene expression from independent CPC donors (CPC1–3; blue bars), two HDF (HDF and F3; red bars), and  two independent MSC (MSC19, MSC4:130' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 5. Validation of putative CPC membrane markers. (a) RT-qPCR of CACNG7, CDH5, FR11, GPR4 gene expression from independent CPC donors (CPC1–3; blue bars), two HDF (HDF and F3; red bars), and  two independent MSC (MSC19, MSC:131' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 6. Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. (a) Scheme for the isolation and expansion of human and porcine CPC. Cells were analyzed in passage 2 (p2) or passage 5 (p5); C:132' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 6. Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. (a) Scheme for the isolation and expansion of human and porcine CPC. Cells were analyzed in passage 2 (p2) or passage 5 (p5); :140' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 6. Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. (a) Scheme for the isolation and expansion of human and porcine CPC. Cells were analyzed in passage 2 (p2) or passage 5 (p5);:141' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 6. Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. (a) Scheme for the isolation and expansion of human and porcine CPC. Cells were analyzed in passage 2 (p2) or passage 5 (p5):142' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 6. Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. (a) Scheme for the isolation and expansion of human and porcine CPC. Cells were analyzed in passage 2 (p2) or passage 5 (p5:143' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 6. Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. (a) Scheme for the isolation and expansion of human and porcine CPC. Cells were analyzed in passage 2 (p2) or passage 5 (:145' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd:Figure 6. Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. (a) Scheme for the isolation and expansion of human and porcine CPC. Cells were analyzed in passage 2 (p2) or passage 5 :148' cannot be found Offset: 2608974 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_39571.indd: 25. Sanz-Ruiz, R. et al. Rationale and Design of a Clinical Trial to Evaluate the Safety and Efficacy of Intracoronary Infusion of Allogeneic Human Cardiac Stem Cells in Patients With Acute Myocardial Infarction and Left Ventricular Dysfunc:150' cannot be found Offset: 2608974 MIMEtype: application/pdf PDFMetadata: Objects: 612 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic an ... Children: Item: Title: Results and Discussion Children: Item: Title: Deep comparative transcriptome analysis of CPC by mRNA sequencing. Item: Title: nLC-MS/MS-based comparative proteomic analysis of CPC combined with ITRAQ. Item: Title: Data integration of the two large-scale techniques. Item: Title: CPC surface markers. Item: Title: Validation of markers for human CPC. Item: Title: Conclusions Item: Title: Methods Children: Item: Title: Cell culture. Item: Title: Label-free proteomics analysis. Item: Title: iTRAQ labeling and quantitative proteomics. Item: Title: Systems biology. Item: Title: Flow cytometry. Item: Title: Western blotting. Item: Title: Immunofluorescence. Item: Title: mRNAseq analysis. Item: Title: RT-qPCR. Item: Title: Statistics. Item: Title: Acknowledgements Item: Title: Figure 1 RNAseq analysis of CPC compared with MSC and HDF. Item: Title: Figure 2 Distribution and classification of CPC proteome compared with MSC and HDF proteomes. Item: Title: Figure 3 Validation of proteins identified by comparative proteomics and system biology analysis. Item: Title: Figure 4 Definition of the minimal core of preferentially expressed plasma membrane proteins in CPC compared with MSC and HDF. Item: Title: Figure 5 Validation of putative CPC membrane markers. Item: Title: Figure 6 Validation of highly differentially expressed membrane proteins in CPC at early passages after isolation. Item: Title: Table 1 Main CPC membrane proteins differentially expressed in comparison with MSC. 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doi:10.1038/s41598-019-39571-x Springer US Scientific Reports, doi:10.1038/s41598-019-39571-x Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization Josamp#x000E9; Luis Toramp#x000E1;n Juan Antonio Lamp#x000F3;pez Patricia Gomes-Alves Susana Aguilar Carlos Torroja Marco Trevisan-Herraz Isabel Moscoso Maria Joamp#x000E3;o Sebastiamp#x000E3;o Margarida Serra Catarina Brito Francisco Miguel Cruz Juan Carlos Sepamp#x000FA;lveda Josamp#x000E9; Luis Abad Carlos Galamp#x000E1;n-Arriola Borja Ibanez Fernando Martamp#x000ED;nez Maramp#x000ED;a Eugenia Fernamp#x000E1;ndez Francisco Fernamp#x000E1;ndez-Aviles Itziar Palacios Luis R-Borlado Jesamp#x000FA;s Vamp#x000E1;zquez Paula M. Alves Antonio Bernad 10.1038/s41598-019-39571-x http://dx.doi.org/10.1038/s41598-019-39571-x journal Scientific Reports © 2019, The Author(s) 2045-2322 10.1038/s41598-019-39571-x Springer 2019-03-11T11:33:02+01:00 2019-03-07T12:18:50+05:30 2019-03-11T11:33:02+01:00 True iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) VoR uuid:a62d0215-95b7-4510-97b3-c04879399ea3 uuid:c0582732-a136-4d2f-8453-817a67e09853 default 1 converted uuid:e1beff08-87a8-4d16-9c82-059bc71b5bf7 converted to PDF/A-2b pdfToolbox 2019-03-07T12:19:28+05:30 2 B http://ns.adobe.com/xap/1.0/mm/ xmpMM XMP Media Management Schema internal UUID based identifier for specific incarnation of a document InstanceID URI internal The common identifier for all versions and renditions of a document. OriginalDocumentID URI http://ns.adobe.com/pdf/1.3/ pdf Adobe PDF Schema internal A name object indicating whether the document has been modified to include trapping information Trapped Text http://ns.adobe.com/pdfx/1.3/ pdfx pdfx internal ID of PDF/X standard GTS_PDFXVersion Text internal Conformance level of PDF/X standard GTS_PDFXConformance Text internal Company creating the PDF Company Text internal Date when document was last modified SourceModified Text internal Mirrors crossmark:DOI doi Text http://www.aiim.org/pdfa/ns/id/ pdfaid PDF/A ID Schema internal Part of PDF/A standard part Integer internal Amendment of PDF/A standard amd Text internal Conformance level of PDF/A standard conformance Text http://prismstandard.org/namespaces/basic/2.0/ prism Prism external The aggregation type specifies the unit of aggregation for a content collection. Comment PRISM recommends that the PRISM Aggregation Type Controlled Vocabulary be used to provide values for this element. Note: PRISM recommends against the use of the #other value currently allowed in this controlled vocabulary. In lieu of using #other please reach out to the PRISM group at info@prismstandard.org to request addition of your term to the Aggregation Type Controlled Vocabulary. aggregationType Text external Copyright copyright Text external The Digital Object Identifier for the article. The DOI may also be used as the dc:identifier. If used as a dc:identifier, the URI form should be captured, and the bare identifier should also be captured using prism:doi. If an alternate unique identifier is used as the required dc:identifier, then the DOI should be specified as a bare identifier within prism:doi only. If the URL associated with a DOI is to be specified, then prism:url may be used in conjunction with prism:doi in order to provide the service endpoint (i.e. the URL). doi Text external ISSN for an electronic version of the issue in which the resource occurs. 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