4239f78f856101545abc10aac91518cd pmc_30886213.pdf d01e65cfab3c0e6e04f65f07704904b25826f37e pmc_30886213.pdf 7994d03b8b73592c9c40d9d220a395b362a6ac9509af0f16d271dc4f3a8727b3 pmc_30886213.pdf Title: Distinct triterpene synthases in the laticifers of Euphorbia lathyris Subject: Scientific Reports, doi:10.1038/s41598-019-40905-y Keywords: Author: Edith Forestier Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Mon Mar 4 19:06:32 2019 CET ModDate: Wed Mar 13 18:36:58 2019 CET Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 3979288 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FPGJWO+Corbel-Bold CID TrueType Identity-H yes yes yes 111 0 NOKNCL+Corbel-BoldItalic Type 1C WinAnsi yes yes yes 75 0 MinionPro-It Type 1C Custom yes no yes 79 0 ICFEMY+Corbel-Bold2 Type 1C WinAnsi yes yes yes 78 0 MinionPro-Regular Type 1C Custom yes no yes 77 0 XFQODD+Corbel2 Type 1C WinAnsi yes yes yes 80 0 HCJAYK+Corbel CID TrueType Identity-H yes yes yes 81 0 MyriadPro-It Type 1C WinAnsi yes no yes 82 0 UGDYYK+TimesNewRomanPSMT Type 1C WinAnsi yes yes yes 416 0 XFQODD+Corbel2 Type 1C WinAnsi yes yes yes 318 0 EuclidSymbol Type 1C Custom yes no yes 76 0 MinionPro-Bold Type 1C WinAnsi yes no yes 112 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:49:53 CEST RepresentationInformation: pmc_30886213.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-03-02 04:24:22 CET Size: 3979288 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 3. Metcalfe, C. R. Distribution of latex in the plant kingdom. Econ. Bot. 21, 115–127 (1967). :28' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 23. Eschenmoser, A., RužiŜ爀欀愀Ⰰ 䰀⸀Ⰰ 䨀攀最攀爀Ⰰ 伀⸀ ☀ 䄀爀椀最漀渀椀Ⰰ 䐀⸀ 娀甀爀 䬀攀渀渀琀渀椀猀 搀攀爀 吀爀椀琀攀爀瀀攀渀攀⸀ ㄀㤀　⸀ 䴀椀琀琀攀椀氀甀渀最⸀ 䔀椀渀攀 猀琀攀爀攀漀挀栀攀洀椀猀挀栀攀 䤀渀琀攀爀瀀爀攀琀愀琀椀漀渀 搀攀爀 戀椀漀最攀渀攀琀椀猀挀栀攀渀 䤀猀漀瀀爀攀渀爀攀最攀氀 戀攀椀 搀攀渀 吀爀椀琀攀爀瀀攀渀攀渀⸀⃾＀䠀攀氀瘀⸀ 䌀栀椀洀⸀ 䄀挀琀懾＀⃾＀㌀㣾＀Ⰰ ㄀㠀㤀〠ጀ㄀㤀　㐀 ㄀㤀㔀㔀⻾＀㨀㐀㤩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㔱㈮㘷㤠㘶㐮㠸‵ㄸ⸳㜳‶㔲⸶㈲崯䠯丯䅐㰼⽎‴㐠〠刾㸾㸊敮摯扪਱㐠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㐀　㤀　㔀⸀椀渀搀搀㫾＀￾＀㈀㐀⻾￾＀￾￾￾￾＀䄀戀攀Ⰰ 䤀⸀Ⰰ 刀漀栀洀攀爀Ⰰ 䴀⸀ ☀ 倀爀攀猀琀眀椀挀栀Ⰰ 䜀⸀ 䐀⸀ 䔀渀稀礀洀愀琀椀挀 挀礀挀氀椀稀愀琀椀漀渀 漀昀 猀焀甀愀氀攀渀攀 愀渀搀 漀砀椀搀漀猀焀甀愀氀攀渀攀 琀漀 猀琀攀爀漀氀猀 愀渀搀 琀爀椀琀攀爀瀀攀渀攀猀⸀⃾＀䌀栀攀洀⸀ 刀攀瘀⻾＀⃾＀㤀㏾＀Ⰰ ㈀㄀㠀㤠ጀ㈀㈀　㘀 ㄀㤀㤀㌀⻾＀㨀㔀〩⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈳㠮㔶㈠㘳㐮㜶㌠㈴㐮ㄴ‶㈲⸶㈲崯䠯丯䅐㰼⽎‴㔠〠刾㸾㸊敮摯扪਱㔠〠潢樊㰼⽆‴⽔祰支䅮湯琯䑥獴⣾＀匀瀀爀椀渀最攀爀一愀琀甀爀攀开匀挀椀刀攀瀀开㐀　㤀　㔀⸀椀渀搀搀㫾＀￾＀㌀㌀⻾￾＀￾￾￾￾＀吀栀漀洀愀Ⰰ 刀⸀⃾＀攀琀 愀泾＀⸀ 䤀渀猀椀最栀琀 椀渀琀漀 猀琀攀爀漀椀搀 猀挀愀昀昀漀氀搀 昀漀爀洀愀琀椀漀渀 昀爀漀洀 琀栀攀 猀琀爀甀挀琀甀爀攀 漀昀 栀甀洀愀渀 漀砀椀搀漀猀焀甀愀氀攀渀攀 挀礀挀氀愀猀攀⸀⃾＀一愀琀甀爀旾＀⃾＀㐀㌀㋾＀Ⰰ ㄀㄀㠠ጀ㄀㈀㈀ ㈀　　㈀⻾＀㨀㔀ㄩ⽂潲摥牛〠〠そ⽓畢瑹灥⽌楮欯䉓㰼⽗‰⽔祰支䉯牤敲⽓⽓㸾⽒散瑛㈴㜮ㄶ㌠㘳㐮㜶㌠㈵㈮㜴ㄠ㘲㈮㘲㉝⽈⽎⽁值㰯丠㐶‰⁒㸾㸾੥湤潢樊ㄶ‰扪਼㰯䘠㐯呹灥⽁湮潴⽄敳琨SpringerNature_SciRep_40905.indd: 34. Phillips, D. R., Rasbery, J. M., Bartel, B. & Matsuda, S. P. Biosynthetic diversity in plant triterpene cyclization. Curr. Opin. Plant Biol. 9, 305–314 (2006). :52' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 24. Abe, I., Rohmer, M. & Prestwich, G. D. Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes. Chem. Rev. 93, 2189–2206 (1993). :50' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 33. Thoma, R. et al. Insight into steroid scaffold formation from the structure of human oxidosqualene cyclase. Nature 432, 118–122 (2002). :51' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 11. Luo, D. et al. Oxidation and cyclization of casbene in the biosynthesis of Euphorbia factors from mature seeds of Euphorbia lathyris L. Proc. Natl. Acad. Sci. USA 113, E5082–9 (2016). :54' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 24. Abe, I., Rohmer, M. & Prestwich, G. D. Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes. Chem. Rev. 93, 2189–2206 (1993). :55' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 1. Cyclization of 2,3-oxidosqualene into tetracyclic triterpenes. Lanosterol or cycloartenol synthases cyclize the protonated 2,3-oxidosqualene in a chair-boat-chair conformation via a protosteryl cation, whereas butyrospermol or euphol s:59' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 28. Meyer, M. M., Xu, R. & Matsuda, S. P. Directed evolution to generate cycloartenol synthase mutants that produce lanosterol. Org. Lett. 4, 1395–8 (2002). :65' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 2. Alignment of higher plant OSCs implied in tetracyclic triterpene synthesis. Multiple sequence alignments were performed with MacVector software using the ClustalW program and BLOSUM 62 matrix. Important conserved or variable amino acid:79' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 2. Alignment of higher plant OSCs implied in tetracyclic triterpene synthesis. Multiple sequence alignments were performed with MacVector software using the ClustalW program and BLOSUM 62 matrix. Important conserved or variable amino aci:80' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 3. Maximum likelihood phylogenetic tree based on amino acid sequences of squalene-hopene synthase (SHS) and OSCs. Squalene-hopane cyclase accessions: Neosartorya fischeri (DS027688.1), Zymomonas mobilis (X73561), Adiantum capillus-ve:82' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 3. Maximum likelihood phylogenetic tree based on amino acid sequences of squalene-hopene synthase (SHS) and OSCs. Squalene-hopane cyclase accessions: Neosartorya fischeri (DS027688.1), Zymomonas mobilis (X73561), Adiantum capillus-v:83' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 24. Abe, I., Rohmer, M. & Prestwich, G. D. Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes. Chem. Rev. 93, 2189–2206 (1993). :72' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 28. Meyer, M. M., Xu, R. & Matsuda, S. P. Directed evolution to generate cycloartenol synthase mutants that produce lanosterol. Org. Lett. 4, 1395–8 (2002). :84' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 32. Wendt, K. U., Poralla, K. & Schulz, G. E. Structure and function of a squalene cyclase. Science 277, 1811–1815 (1997). :86' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 33. Thoma, R. et al. Insight into steroid scaffold formation from the structure of human oxidosqualene cyclase. Nature 432, 118–122 (2002). :87' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 2. Alignment of higher plant OSCs implied in tetracyclic triterpene synthesis. Multiple sequence alignments were performed with MacVector software using the ClustalW program and BLOSUM 62 matrix. Important conserved or variable amino ac:91' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 33. Thoma, R. et al. Insight into steroid scaffold formation from the structure of human oxidosqualene cyclase. Nature 432, 118–122 (2002). :95' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 39. Kolesnikova, M. D., Xiong, Q., Lodeiro, S., Hua, L. & Matsuda, S. P. Lanosterol biosynthesis in plants. Arch. Biochem. Biophys. 447, 87–95 (2006). :97' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 40. Suzuki, M. et al. Lanosterol synthase in dicotyledonous plants. Plant Cell. Physiol. 47, 565–571 (2006). :99' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 25. Corey, E. J., Matsuda, S. P. & Bartel, B. Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen. Proc. Natl.:101' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 45. Lodeiro, S. et al. An oxidosqualene cyclase makes numerous products by diverse mechanisms: a challenge to prevailing concepts of triterpene biosynthesis. J. Am. Chem. Soc. 129, 11213–11222 (2007). :103' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 52. Morlacchi, P. et al. Product profile of PEN3: the last unexamined oxidosqualene cyclase in Arabidopsis thaliana. Org. Lett. 11, 2627–2630 (2009). :105' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 4. Chromatograms (TIC, GC-MS) showing the triterpene profiles of the yeast erg7 transformed with triterpene synthases from Euphorbia lathyris. (A) erg7::ElLAS1; (B) erg7::ElBUT1; (C) erg7::ElCAS1; (D) latex triterpenes s:112' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 4. Chromatograms (TIC, GC-MS) showing the triterpene profiles of the yeast erg7 transformed with triterpene synthases from Euphorbia lathyris. (A) erg7::ElLAS1; (B) erg7::ElBUT1; (C) erg7::ElCAS1; (D) latex triterpenes :113' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd: 22. Xu, R., Fazio, G. C. & Matsuda, S. P. On the origins of triterpenoid skeletal diversity. Phytochemistry 65, 261–291 (2004). :114' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 4. Chromatograms (TIC, GC-MS) showing the triterpene profiles of the yeast erg7 transformed with triterpene synthases from Euphorbia lathyris. (A) erg7::ElLAS1; (B) erg7::ElBUT1; (C) erg7::ElCAS1; (D) latex triterpenes:115' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 4. Chromatograms (TIC, GC-MS) showing the triterpene profiles of the yeast erg7 transformed with triterpene synthases from Euphorbia lathyris. (A) erg7::ElLAS1; (B) erg7::ElBUT1; (C) erg7::ElCAS1; (D) latex triterpene:116' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 2. Alignment of higher plant OSCs implied in tetracyclic triterpene synthesis. Multiple sequence alignments were performed with MacVector software using the ClustalW program and BLOSUM 62 matrix. Important conserved or variable amino a:107' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 2. Alignment of higher plant OSCs implied in tetracyclic triterpene synthesis. Multiple sequence alignments were performed with MacVector software using the ClustalW program and BLOSUM 62 matrix. Important conserved or variable amino :108' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 5. Chromatograms (TIC, GC-MS) displaying the triterpene profile of Nicotiana benthamiana leaf tissues after in folia agroinfiltration of ElBUT1. (A) TLC-purified triterpene fraction of leaves infiltrated with T-DNA constructs driv:119' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 5. Chromatograms (TIC, GC-MS) displaying the triterpene profile of Nicotiana benthamiana leaf tissues after in folia agroinfiltration of ElBUT1. (A) TLC-purified triterpene fraction of leaves infiltrated with T-DNA constructs dri:120' cannot be found Offset: 3416829 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'SpringerNature_SciRep_40905.indd:Figure 5. Chromatograms (TIC, GC-MS) displaying the triterpene profile of Nicotiana benthamiana leaf tissues after in folia agroinfiltration of ElBUT1. (A) TLC-purified triterpene fraction of leaves infiltrated with T-DNA constructs dr:121' cannot be found Offset: 3416829 MIMEtype: application/pdf PDFMetadata: Objects: 501 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Distinct triterpene synthases in the laticifers of Euphorbia lathyris Children: Item: Title: Results and Discussion Children: Item: Title: A triterpene “lathyris” signature. Item: Title: Latex-specific triterpene synthases. Item: Title: Functional analysis of the latex triterpene synthases. Item: Title: Accuracy of the steroidal triterpene synthases of Euphorbia lathyris. Item: Title: Laticifers as a triterpene factory. Item: Title: Material and Methods Children: Item: Title: Plant material. Item: Title: Generation of latex transcriptome database. Item: Title: Identification and isolation of Euphorbia lathyris oxidosqualene cyclases (OSCs). Item: Title: Yeast expression experiments. Item: Title: In folia expression experiments with Nicotiana benthamiana. Item: Title: Triterpene and sterol analysis. Item: Title: Accession numbers. Item: Title: Acknowledgements Item: Title: Figure 1 Cyclization of 2,3-oxidosqualene into tetracyclic triterpenes. Item: Title: Figure 2 Alignment of higher plant OSCs implied in tetracyclic triterpene synthesis. Item: Title: Figure 3 Maximum likelihood phylogenetic tree based on amino acid sequences of squalene-hopene synthase (SHS) and OSCs. Item: Title: Figure 4 Chromatograms (TIC, GC-MS) showing the triterpene profiles of the yeast erg7 transformed with triterpene synthases from Euphorbia lathyris. Item: Title: Figure 5 Chromatograms (TIC, GC-MS) displaying the triterpene profile of Nicotiana benthamiana leaf tissues after in folia agroinfiltration of ElBUT1. Info: Title: Distinct triterpene synthases in the laticifers of Euphorbia lathyris Author: Edith Forestier Subject: Scientific Reports, doi:10.1038/s41598-019-40905-y Keywords: Creator: Springer Producer: iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Mon Mar 04 19:36:32 CET 2019 ModDate: Wed Mar 13 18:36:58 CET 2019 ID: 0x7955febbb99a5fc83ac1904836345e6c, 0xcdf6fec5ec6300493c2bd9724ed247e9 Filters: FilterPipeline: FlateDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1100 ImageHeight: 90 ColorSpace: black is zero BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1350 ImageHeight: 1883 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1650 ImageHeight: 1275 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1000 ImageHeight: 1341 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 850 ImageHeight: 2253 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 850 ImageHeight: 1665 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Fonts: Type0: Font: BaseFont: HCJAYK+Corbel Encoding: Identity-H ToUnicode: true Font: BaseFont: FPGJWO+Corbel-Bold Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: XFQODD+Corbel2 FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: XFQODD+Corbel2 Flags: Nonsymbolic FontBBox: -553, -239, 1110, 952 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: UGDYYK+TimesNewRomanPSMT FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: UGDYYK+TimesNewRomanPSMT Flags: Serif, Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-Bold FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Bold Flags: Serif, Nonsymbolic FontBBox: -319, -360, 1684, 1024 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MyriadPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MyriadPro-It Flags: Nonsymbolic, Italic FontBBox: -185, -250, 1090, 953 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: NOKNCL+Corbel-BoldItalic FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: NOKNCL+Corbel-BoldItalic Flags: Nonsymbolic, Italic FontBBox: -494, -239, 1194, 952 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: EuclidSymbol FirstChar: 0 LastChar: 255 FontDescriptor: FontName: EuclidSymbol Flags: Symbolic FontBBox: -166, -253, 1090, 950 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MinionPro-Regular FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Regular Flags: Serif, Nonsymbolic FontBBox: -290, -360, 1684, 989 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: ICFEMY+Corbel-Bold2 FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: ICFEMY+Corbel-Bold2 Flags: Nonsymbolic FontBBox: -556, -239, 1155, 952 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-It Flags: Serif, Nonsymbolic, Italic FontBBox: -201, -360, 1684, 1002 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: FPGJWO+Corbel-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: FPGJWO+Corbel-Bold Flags: Symbolic FontBBox: -556, -239, 1155, 952 FontFile2: true Font: BaseFont: HCJAYK+Corbel CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: HCJAYK+Corbel Flags: Symbolic FontBBox: -553, -239, 1110, 952 FontFile2: true XMP: application/pdf doi:10.1038/s41598-019-40905-y Springer US Scientific Reports, doi:10.1038/s41598-019-40905-y Distinct triterpene synthases in the laticifers of Euphorbia lathyris Edith Forestier Carmen Romero-Segura Irini Pateraki Emilio Centeno Vincent Compagnon Myriam Preiss Anne Berna Albert Boronat Thomas J. Bach Sylvain Darnet Hubert Schaller 10.1038/s41598-019-40905-y http://dx.doi.org/10.1038/s41598-019-40905-y journal Scientific Reports © 2019, The Author(s) 2045-2322 10.1038/s41598-019-40905-y Springer 2019-03-13T18:36:58+01:00 2019-03-04T23:36:32+05:30 2019-03-13T18:36:58+01:00 True iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) VoR uuid:0ae57f8a-1c2a-4b43-a08d-3148d953ac74 uuid:85d47349-9ce9-4b77-83af-abfe29e620e0 default 1 converted uuid:74ce3fab-a101-4640-9a54-6f9fe1e243b7 converted to PDF/A-2b pdfToolbox 2019-03-04T23:37:18+05:30 2 B http://ns.adobe.com/pdf/1.3/ pdf Adobe PDF Schema internal A name object indicating whether the document has been modified to include trapping information Trapped Text http://ns.adobe.com/xap/1.0/mm/ xmpMM XMP Media Management Schema internal UUID based identifier for specific incarnation of a document InstanceID URI internal The common identifier for all versions and renditions of a document. 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