b8cc8d308222a6391e081f1f0e1f8bb8 pmc_30922370.pdf fe2c31bbfbcb8dbd453c4590efde3847b5d8a753 pmc_30922370.pdf 32677049765d8a78bba581eeefc7d473ff091f0e3229146918d03b45dc5dffcf pmc_30922370.pdf Title: Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain Subject: Parasites & Vectors, https://doi.org/10.1186/s13071-019-3359-1 Keywords: Aedes albopictus,Zika virus,Spanish populations,Vector competence,Viral copies,RT-qPCR,Plaque assay Author: Mikel A. González Creator: Springer Producer: Acrobat Distiller 10.1.8 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (AGPL-version) CreationDate: Wed Mar 27 15:48:03 2019 CET ModDate: Fri Mar 29 04:28:17 2019 CET Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 790.866 pts Page rot: 0 File size: 1366827 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MyriadPro-Light Type 1C Custom yes no yes 38 0 MyriadPro-It Type 1C Custom yes no yes 32 0 MyriadPro-Semibold Type 1C Custom yes no yes 35 0 MyriadPro-Bold Type 1C WinAnsi yes no yes 33 0 MyriadPro-LightIt Type 1C WinAnsi yes no yes 39 0 TSMJCB+MTSY Type 1C Custom yes yes yes 36 0 MyriadPro-Regular Type 1C Custom yes no yes 37 0 WarnockPro-It Type 1C Custom yes no yes 31 0 WarnockPro-Regular Type 1C Custom yes no yes 34 0 MJUMPV+OpenSans Type 1C WinAnsi yes yes no 251 0 MyriadPro-SemiboldIt Type 1C WinAnsi yes no yes 55 0 FSXBQD+EuclidSymbol Type 1C Custom yes yes yes 56 0 MBUFUV+OregonLDOBookSinistral Type 1C WinAnsi yes yes no 184 0 MBUFUV+OpenSans-Light Type 1C Custom yes yes no 178 0 MBUFUV+OpenSans-Bold Type 1C WinAnsi yes yes no 181 0 MBUFUV+OpenSans-Extrabold Type 1C WinAnsi yes yes no 174 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:49:10 CEST RepresentationInformation: pmc_30922370.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-06 04:14:02 CEST Size: 1366827 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 356 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain Children: Item: Title: Abstract Children: Item: Title: Background: Item: Title: Methods: Item: Title: Results: Item: Title: Conclusions: Item: Title: Background Item: Title: Methods Children: Item: Title: Mosquito populations Item: Title: Virus strain Item: Title: Experiment 1: Vector competence of five Ae. albopictus populations from Spain Item: Title: Experiment 2: Effect of an additional uninfected blood meal on the Ae. albopictus vector competence to ZIKV Item: Title: Sample processing and virus detection by RT-qPCR Item: Title: Collection of saliva and virus detection by plaque assay in Vero cells Item: Title: Viral quantification and statistical analysis Item: Title: Results Children: Item: Title: Indicators of Ae. albopictus infestation Item: Title: Viral competence parameters Item: Title: Viral loads in mosquito tissues Item: Title: Effect of a second blood meal on vector competence Item: Title: Discussion Item: Title: Conclusions Item: Title: Acknowledgements Item: Title: References Info: Title: Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain Author: Mikel A. 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González Márcio G. Pavan Rosilainy S. Fernandes Núria Busquets Mariana R. David Ricardo Lourenço-Oliveira Ana L. 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