0adfc510e7ac32e4a3318371efefb290 pmc_31275156.pdf d4894a3174a70c7dd20ce6c3f440710e97ec649d pmc_31275156.pdf 93e8912d7010e21d3264ccc5bda3cd317c6dbc2d818b19c364c7716f2e7b9645 pmc_31275156.pdf Title: Brain and Pituitary Response to Vaccination in Gilthead Seabream (Sparus aurata L.) Subject: Vaccination is a widely used therapeutical strategy in aquaculture, but whether vaccination elicits stress responses in the central neuroendocrine system and enhances the crosstalk between the immune and endocrine systems in the brain or pituitary after vaccination is unclear. Keywords: brain, pituitary, vaccination, immune response, stress response Author: F. E. Reyes-López and L. Tort Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Sat Jun 15 14:14:18 2019 CEST ModDate: Sat Jun 15 14:14:18 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 3823423 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WQERFV+MinionPro-Regular Type 1 Custom yes yes no 32 0 VYFQHI+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 33 0 YNBLCB+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 34 0 CCVXXJ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 39 0 SDSZMO+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 40 0 FLNLJY+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 41 0 QHWLRN+MTGU Type 1 Builtin yes yes no 42 0 TOMIIF+MinionPro-It Type 1 Custom yes yes no 134 0 PUIKHS+MTSYN Type 1 Builtin yes yes no 187 0 ASGAQK+HelveticaNeueLTStd-Md Type 1 Custom yes yes no 194 0 XODHDR+RBLMI Type 1 Builtin yes yes no 198 0 LLZFTU+MinionPro-Bold Type 1 Custom yes yes no 205 0 XDWDPG+MTSYB Type 1 Builtin yes yes no 225 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:38:34 CEST RepresentationInformation: pmc_31275156.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-06 04:10:09 CEST Size: 3823423 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 760 FreeObjects: 6 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Brain and Pituitary Response to Vaccination in Gilthead Seabream (Sparus aurata L.) Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Fish Husbandry and Experimental Design Item: Title: Vaccines and Sample Collection Children: Item: Title: Experiment 1 Item: Title: Experiment 2 Item: Title: Plasma Isolation and Test of Biochemical Parameters Item: Title: Total RNA Extraction and Reverse Transcription Quantitative Real-Time PCR (RT-qPCR) Item: Title: Statistics Item: Title: Results Children: Item: Title: Brain and Pituitary Stress Response to Bath Vaccine Item: Title: Brain and Pituitary Stress and Immune Responses to Injected Vaccine Item: Title: mRNA Expression of Immune Genes in Brain and Pituitary Item: Title: Discussion Children: Item: Title: Brain and Pituitary Stress Response to Vaccines Item: Title: Immune Gene Response in Brain and Pituitary After Vaccine Injection Item: Title: Conclusion Item: Title: Data Availability Item: Title: Ethics Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: References Info: Title: Brain and Pituitary Response to Vaccination in Gilthead Seabream (Sparus aurata L.) Author: F. E. Reyes-López and L. Tort Subject: Vaccination is a widely used therapeutical strategy in aquaculture, but whether vaccination elicits stress responses in the central neuroendocrine system and enhances the crosstalk between the immune and endocrine systems in the brain or pituitary after vaccination is unclear. Keywords: brain, pituitary, vaccination, immune response, stress response Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Sat Jun 15 14:44:18 CEST 2019 ModDate: Sat Jun 15 14:44:18 CEST 2019 ID: 0xbf362b7a41786928c0e6d0d530fc4c7b, 0xbf362b7a41786928c0e6d0d530fc4c7b Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: WQERFV+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 250 FontDescriptor: FontName: WQERFV+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: VYFQHI+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: VYFQHI+HelveticaNeueLTStd-Bd Flags: Symbolic FontBBox: -166, -218, 1078, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XDWDPG+MTSYB FontSubset: true FirstChar: 48 LastChar: 48 FontDescriptor: FontName: XDWDPG+MTSYB Flags: Symbolic FontBBox: -16, -935, 1043, 896 FontFile: true Font: BaseFont: YNBLCB+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: YNBLCB+HelveticaNeueLTStd-Lt Flags: Symbolic FontBBox: -166, -214, 1050, 967 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: ASGAQK+HelveticaNeueLTStd-Md FontSubset: true FirstChar: 49 LastChar: 120 FontDescriptor: FontName: ASGAQK+HelveticaNeueLTStd-Md Flags: Symbolic FontBBox: -165, -221, 1066, 952 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TOMIIF+MinionPro-It FontSubset: true FirstChar: 2 LastChar: 243 FontDescriptor: FontName: TOMIIF+MinionPro-It Flags: Symbolic FontBBox: -201, -360, 1684, 919 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XODHDR+RBLMI FontSubset: true FirstChar: 60 LastChar: 62 FontDescriptor: FontName: XODHDR+RBLMI Flags: Symbolic FontBBox: -11, -230, 987, 750 FontFile: true Font: BaseFont: CCVXXJ+HelveticaNeueLTStd-BdIt FontSubset: true FirstChar: 42 LastChar: 121 FontDescriptor: FontName: CCVXXJ+HelveticaNeueLTStd-BdIt Flags: Symbolic FontBBox: -166, -218, 1129, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: SDSZMO+HelveticaNeueLTStd-LtIt FontSubset: true FirstChar: 40 LastChar: 243 FontDescriptor: FontName: SDSZMO+HelveticaNeueLTStd-LtIt Flags: Symbolic FontBBox: -165, -214, 1099, 951 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: FLNLJY+HelveticaNeueLTStd-MdIt FontSubset: true FirstChar: 42 LastChar: 243 FontDescriptor: FontName: FLNLJY+HelveticaNeueLTStd-MdIt Flags: Symbolic FontBBox: -165, -218, 1125, 947 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: QHWLRN+MTGU FontSubset: true FirstChar: 97 LastChar: 109 FontDescriptor: FontName: QHWLRN+MTGU Flags: Symbolic FontBBox: 25, -213, 839, 683 FontFile: true Font: BaseFont: LLZFTU+MinionPro-Bold FontSubset: true FirstChar: 3 LastChar: 117 FontDescriptor: FontName: LLZFTU+MinionPro-Bold Flags: Symbolic FontBBox: -318, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: PUIKHS+MTSYN FontSubset: true FirstChar: 0 LastChar: 21 FontDescriptor: FontName: PUIKHS+MTSYN Flags: Symbolic FontBBox: 0, -954, 1043, 900 FontFile: true XMP: brain, pituitary, vaccination, immune response, stress response pdfTeX-1.40.15 1.5 application/pdf Brain and Pituitary Response to Vaccination in Gilthead Seabream (Sparus aurata L.) Vaccination is a widely used therapeutical strategy in aquaculture, but whether vaccination elicits stress responses in the central neuroendocrine system and enhances the crosstalk between the immune and endocrine systems in the brain or pituitary after vaccination is unclear. F. E. Reyes-López and L. Tort brain pituitary vaccination immune response stress response 2019-06-15T17:44:18+05:30 fphys-10-00717.tex 2019-06-15T17:44:18+05:30 2019-06-15T17:44:18+05:30 2019-06-15T17:44:18+05:30 LaTeX with hyperref package uuid:d7f7fd13-9bbb-4620-8aea-88820a88d188 uuid:1882b133-3905-4fd7-b7f2-054c4cea33ef Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 43, 718, 147, 754 Annotation: Subtype: Link Rect: 474, 736, 551, 745 Annotation: Subtype: Link Rect: 474, 727, 551, 737 Annotation: Subtype: Link Rect: 453, 718, 551, 729 Annotation: Subtype: Link Rect: 86, 435, 160, 445 Annotation: Subtype: Link Rect: 75, 66, 160, 74 Annotation: Subtype: Link Rect: 521, 677, 551, 707 Annotation: Subtype: Link Rect: 176, 577, 551, 645 Annotation: Subtype: Link Rect: 176, 557, 213, 567 Annotation: Subtype: Link Rect: 227, 557, 288, 567 Annotation: Subtype: Link Rect: 297, 557, 334, 567 Annotation: Subtype: Link Rect: 340, 557, 436, 567 Annotation: Subtype: Link Rect: 443, 557, 493, 567 Annotation: Subtype: Link Rect: 176, 546, 237, 558 Annotation: Subtype: Link Rect: 244, 546, 320, 558 Annotation: Subtype: Link Rect: 346, 546, 374, 558 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 202, 649, 265, 660 ActionDest: 605 Annotation: Subtype: Link Rect: 270, 649, 290, 660 ActionDest: 605 Annotation: Subtype: Link Rect: 43, 637, 120, 648 ActionDest: 562 Annotation: Subtype: Link Rect: 127, 637, 148, 648 ActionDest: 562 Annotation: Subtype: Link Rect: 189, 557, 236, 568 ActionDest: 496 Annotation: Subtype: Link Rect: 244, 557, 264, 568 ActionDest: 496 Annotation: Subtype: Link Rect: 113, 523, 190, 534 ActionDest: 496 Annotation: Subtype: Link Rect: 196, 523, 216, 534 ActionDest: 496 Annotation: Subtype: Link Rect: 47, 477, 108, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 113, 477, 133, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 138, 477, 186, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 191, 477, 211, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 130, 419, 210, 431 ActionDest: 496 Annotation: Subtype: Link Rect: 216, 419, 236, 431 ActionDest: 496 Annotation: Subtype: Link Rect: 242, 419, 292, 431 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 409, 70, 419 ActionDest: 562 Annotation: Subtype: Link Rect: 75, 409, 95, 419 ActionDest: 562 Annotation: Subtype: Link Rect: 99, 409, 206, 419 ActionDest: 562 Annotation: Subtype: Link Rect: 210, 409, 231, 419 ActionDest: 562 Annotation: Subtype: Link Rect: 105, 363, 159, 374 ActionDest: 496 Annotation: Subtype: Link Rect: 164, 363, 185, 374 ActionDest: 496 Annotation: Subtype: Link Rect: 190, 363, 292, 374 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 351, 63, 362 ActionDest: 562 Annotation: Subtype: Link Rect: 67, 351, 88, 362 ActionDest: 562 Annotation: Subtype: Link Rect: 138, 340, 157, 351 ActionDest: 562 Annotation: Subtype: Link Rect: 162, 340, 182, 351 ActionDest: 562 Annotation: Subtype: Link Rect: 180, 316, 246, 328 ActionDest: 562 Annotation: Subtype: Link Rect: 252, 316, 272, 328 ActionDest: 562 Annotation: Subtype: Link Rect: 148, 271, 196, 282 ActionDest: 562 Annotation: Subtype: Link Rect: 202, 271, 222, 282 ActionDest: 562 Annotation: Subtype: Link Rect: 104, 248, 212, 259 ActionDest: 496 Annotation: Subtype: Link Rect: 215, 248, 235, 259 ActionDest: 496 Annotation: Subtype: Link Rect: 47, 214, 116, 225 ActionDest: 496 Annotation: Subtype: Link Rect: 118, 214, 139, 225 ActionDest: 496 Annotation: Subtype: Link Rect: 47, 180, 122, 190 ActionDest: 562 Annotation: Subtype: Link Rect: 127, 180, 148, 190 ActionDest: 562 Annotation: Subtype: Link Rect: 99, 134, 164, 144 ActionDest: 562 Annotation: Subtype: Link Rect: 171, 134, 191, 144 ActionDest: 562 Annotation: Subtype: Link Rect: 256, 87, 292, 99 ActionDest: 496 Annotation: Subtype: Link Rect: 43, 76, 67, 87 ActionDest: 496 Annotation: Subtype: Link Rect: 73, 76, 93, 87 ActionDest: 496 Annotation: Subtype: Link Rect: 494, 683, 549, 694 ActionDest: 562 Annotation: Subtype: Link Rect: 302, 671, 322, 683 ActionDest: 562 Annotation: Subtype: Link Rect: 329, 671, 395, 683 ActionDest: 562 Annotation: Subtype: Link Rect: 402, 671, 422, 683 ActionDest: 562 Annotation: Subtype: Link Rect: 355, 637, 388, 648 ActionDest: 562 Annotation: Subtype: Link Rect: 394, 637, 414, 648 ActionDest: 562 Annotation: Subtype: Link Rect: 419, 637, 479, 648 ActionDest: 496 Annotation: Subtype: Link Rect: 484, 637, 504, 648 ActionDest: 496 Annotation: Subtype: Link Rect: 305, 591, 360, 603 ActionDest: 496 Annotation: Subtype: Link Rect: 362, 591, 382, 603 ActionDest: 496 Annotation: Subtype: Link Rect: 350, 557, 425, 568 ActionDest: 562 Annotation: Subtype: Link Rect: 429, 557, 450, 568 ActionDest: 562 Annotation: Subtype: Link Rect: 520, 546, 551, 557 ActionDest: 496 Annotation: Subtype: Link Rect: 302, 535, 325, 545 ActionDest: 496 Annotation: Subtype: Link Rect: 331, 535, 351, 545 ActionDest: 496 Annotation: Subtype: Link Rect: 472, 523, 522, 534 ActionDest: 496 Annotation: Subtype: Link Rect: 528, 523, 549, 534 ActionDest: 496 Annotation: Subtype: Link Rect: 302, 512, 360, 522 ActionDest: 562 Annotation: Subtype: Link Rect: 365, 512, 385, 522 ActionDest: 562 Annotation: Subtype: Link Rect: 305, 477, 369, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 375, 477, 395, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 401, 477, 463, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 469, 477, 489, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 338, 408, 378, 419 ActionDest: 562 Annotation: Subtype: Link Rect: 383, 408, 403, 419 ActionDest: 562 Annotation: Subtype: Link Rect: 305, 386, 340, 396 ActionDest: 562 Annotation: Subtype: Link Rect: 342, 386, 363, 396 ActionDest: 562 Annotation: Subtype: Link Rect: 428, 99, 490, 110 ActionDest: 562 Annotation: Subtype: Link Rect: 493, 99, 513, 110 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 47, 347, 89, 359 ActionDest: 496 Annotation: Subtype: Link Rect: 91, 347, 112, 359 ActionDest: 496 Annotation: Subtype: Link Rect: 490, 312, 549, 323 ActionDest: 562 Annotation: Subtype: Link Rect: 302, 300, 322, 312 ActionDest: 562 Annotation: Subtype: Link Rect: 458, 64, 488, 76 ActionDest: 222 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 223, 321, 245, 333 ActionDest: 562 Annotation: Subtype: Link Rect: 250, 321, 270, 333 ActionDest: 562 Annotation: Subtype: Link Rect: 246, 264, 276, 275 ActionDest: 222 Annotation: Subtype: Link Rect: 302, 308, 338, 319 ActionDest: 222 Annotation: Subtype: Link Rect: 574, 127, 576, 140 ActionDest: 5 Annotation: Subtype: Link Rect: 574, 127, 576, 140 ActionDest: 5 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 134, 545, 168, 557 ActionDest: 259 Annotation: Subtype: Link Rect: 82, 465, 117, 477 ActionDest: 279 Annotation: Subtype: Link Rect: 341, 568, 376, 580 ActionDest: 279 Annotation: Subtype: Link Rect: 415, 463, 449, 475 ActionDest: 299 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 123, 134, 158, 145 ActionDest: 299 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 121, 85, 161, 96 ActionDest: 337 Annotation: Subtype: Link Rect: 334, 73, 369, 85 ActionDest: 337 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 178, 423, 215, 434 ActionDest: 381 Annotation: Subtype: Link Rect: 497, 354, 549, 365 ActionDest: 496 Annotation: Subtype: Link Rect: 302, 343, 322, 354 ActionDest: 496 Annotation: Subtype: Link Rect: 329, 343, 397, 354 ActionDest: 562 Annotation: Subtype: Link Rect: 403, 343, 423, 354 ActionDest: 562 Annotation: Subtype: Link Rect: 473, 320, 522, 331 ActionDest: 496 Annotation: Subtype: Link Rect: 528, 320, 549, 331 ActionDest: 496 Annotation: Subtype: Link Rect: 302, 308, 356, 320 ActionDest: 562 Annotation: Subtype: Link Rect: 359, 308, 380, 320 ActionDest: 562 Annotation: Subtype: Link Rect: 420, 274, 523, 285 ActionDest: 562 Annotation: Subtype: Link Rect: 528, 274, 549, 285 ActionDest: 562 Annotation: Subtype: Link Rect: 302, 262, 358, 274 ActionDest: 562 Annotation: Subtype: Link Rect: 360, 262, 380, 274 ActionDest: 562 Annotation: Subtype: Link Rect: 305, 145, 366, 156 ActionDest: 562 Annotation: Subtype: Link Rect: 368, 145, 389, 156 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 227, 434, 266, 445 ActionDest: 337 Annotation: Subtype: Link Rect: 268, 434, 275, 445 ActionDest: 381 Annotation: Subtype: Link Rect: 83, 353, 152, 365 ActionDest: 562 Annotation: Subtype: Link Rect: 157, 353, 177, 365 ActionDest: 562 Annotation: Subtype: Link Rect: 199, 353, 262, 365 ActionDest: 562 Annotation: Subtype: Link Rect: 267, 353, 287, 365 ActionDest: 562 Annotation: Subtype: Link Rect: 155, 227, 209, 239 ActionDest: 496 Annotation: Subtype: Link Rect: 213, 227, 233, 239 ActionDest: 496 Annotation: Subtype: Link Rect: 180, 217, 232, 227 ActionDest: 496 Annotation: Subtype: Link Rect: 236, 217, 256, 227 ActionDest: 496 Annotation: Subtype: Link Rect: 261, 217, 292, 227 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 205, 66, 216 ActionDest: 562 Annotation: Subtype: Link Rect: 71, 205, 91, 216 ActionDest: 562 Annotation: Subtype: Link Rect: 96, 205, 162, 216 ActionDest: 605 Annotation: Subtype: Link Rect: 167, 205, 188, 216 ActionDest: 605 Annotation: Subtype: Link Rect: 106, 170, 170, 181 ActionDest: 496 Annotation: Subtype: Link Rect: 176, 170, 196, 181 ActionDest: 496 Annotation: Subtype: Link Rect: 139, 136, 228, 147 ActionDest: 496 Annotation: Subtype: Link Rect: 232, 136, 252, 147 ActionDest: 496 Annotation: Subtype: Link Rect: 255, 136, 292, 147 ActionDest: 605 Annotation: Subtype: Link Rect: 43, 124, 65, 136 ActionDest: 605 Annotation: Subtype: Link Rect: 70, 124, 91, 136 ActionDest: 605 Annotation: Subtype: Link Rect: 47, 80, 136, 90 ActionDest: 562 Annotation: Subtype: Link Rect: 138, 80, 158, 90 ActionDest: 562 Annotation: Subtype: Link Rect: 341, 399, 372, 411 ActionDest: 496 Annotation: Subtype: Link Rect: 375, 399, 395, 411 ActionDest: 496 Annotation: Subtype: Link Rect: 415, 365, 471, 376 ActionDest: 496 Annotation: Subtype: Link Rect: 475, 365, 495, 376 ActionDest: 496 Annotation: Subtype: Link Rect: 323, 331, 373, 342 ActionDest: 496 Annotation: Subtype: Link Rect: 377, 331, 397, 342 ActionDest: 496 Annotation: Subtype: Link Rect: 305, 274, 381, 284 ActionDest: 562 Annotation: Subtype: Link Rect: 383, 274, 404, 284 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 253, 500, 292, 511 ActionDest: 496 Annotation: Subtype: Link Rect: 43, 488, 64, 500 ActionDest: 496 Annotation: Subtype: Link Rect: 66, 488, 86, 500 ActionDest: 496 Annotation: Subtype: Link Rect: 89, 488, 159, 500 ActionDest: 562 Annotation: Subtype: Link Rect: 162, 488, 182, 500 ActionDest: 562 Annotation: Subtype: Link Rect: 185, 488, 242, 500 ActionDest: 562 Annotation: Subtype: Link Rect: 244, 488, 265, 500 ActionDest: 562 Annotation: Subtype: Link Rect: 267, 488, 292, 500 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 477, 64, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 67, 477, 87, 488 ActionDest: 562 Annotation: Subtype: Link Rect: 239, 397, 290, 408 ActionDest: 605 Annotation: Subtype: Link Rect: 43, 385, 64, 396 ActionDest: 605 Annotation: Subtype: Link Rect: 67, 385, 144, 396 ActionDest: 605 Annotation: Subtype: Link Rect: 148, 385, 168, 396 ActionDest: 605 Annotation: Subtype: Link Rect: 47, 339, 91, 351 ActionDest: 562 Annotation: Subtype: Link Rect: 92, 339, 113, 351 ActionDest: 562 Annotation: Subtype: Link Rect: 114, 339, 207, 351 ActionDest: 562 Annotation: Subtype: Link Rect: 209, 339, 229, 351 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 271, 102, 282 ActionDest: 605 Annotation: Subtype: Link Rect: 105, 271, 125, 282 ActionDest: 605 Annotation: Subtype: Link Rect: 84, 236, 132, 247 ActionDest: 562 Annotation: Subtype: Link Rect: 133, 236, 154, 247 ActionDest: 562 Annotation: Subtype: Link Rect: 156, 236, 235, 247 ActionDest: 496 Annotation: Subtype: Link Rect: 237, 236, 257, 247 ActionDest: 496 Annotation: Subtype: Link Rect: 259, 236, 292, 247 ActionDest: 562 Annotation: Subtype: Link Rect: 43, 226, 88, 236 ActionDest: 562 Annotation: Subtype: Link Rect: 93, 226, 114, 236 ActionDest: 562 Annotation: Subtype: Link Rect: 235, 99, 290, 110 ActionDest: 496 Annotation: Subtype: Link Rect: 43, 87, 64, 99 ActionDest: 496 Annotation: Subtype: Link Rect: 411, 706, 447, 717 ActionDest: 562 Annotation: Subtype: Link Rect: 450, 706, 470, 717 ActionDest: 562 Annotation: Subtype: Link Rect: 385, 545, 431, 557 ActionDest: 605 Annotation: Subtype: Link Rect: 436, 545, 456, 557 ActionDest: 605 Annotation: Subtype: Link Rect: 461, 545, 523, 557 ActionDest: 562 Annotation: Subtype: Link Rect: 528, 545, 549, 557 ActionDest: 562 Annotation: Subtype: Link Rect: 302, 534, 371, 545 ActionDest: 562 Annotation: Subtype: Link Rect: 375, 534, 396, 545 ActionDest: 562 Annotation: Subtype: Link Rect: 468, 511, 523, 522 ActionDest: 496 Annotation: Subtype: Link Rect: 525, 511, 545, 522 ActionDest: 496 Annotation: Subtype: Link Rect: 305, 385, 360, 396 ActionDest: 605 Annotation: Subtype: Link Rect: 362, 385, 383, 396 ActionDest: 605 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 240, 392, 293, 402 Annotation: Subtype: Link Rect: 53, 385, 86, 391 Annotation: Subtype: Link Rect: 250, 365, 293, 373 Annotation: Subtype: Link Rect: 53, 356, 88, 363 Annotation: Subtype: Link Rect: 251, 317, 293, 326 Annotation: Subtype: Link Rect: 53, 309, 93, 316 Annotation: Subtype: Link Rect: 53, 270, 166, 279 Annotation: Subtype: Link Rect: 230, 241, 293, 250 Annotation: Subtype: Link Rect: 53, 233, 117, 241 Annotation: Subtype: Link Rect: 181, 203, 293, 212 Annotation: Subtype: Link Rect: 53, 195, 71, 202 Annotation: Subtype: Link Rect: 87, 156, 200, 165 Annotation: Subtype: Link Rect: 53, 127, 153, 137 Annotation: Subtype: Link Rect: 189, 99, 293, 108 Annotation: Subtype: Link Rect: 53, 91, 71, 98 Annotation: Subtype: Link Rect: 409, 421, 498, 430 Annotation: Subtype: Link Rect: 377, 392, 469, 402 Annotation: Subtype: Link Rect: 337, 355, 468, 364 Annotation: Subtype: Link Rect: 384, 308, 497, 316 Annotation: Subtype: Link Rect: 420, 289, 489, 297 Annotation: Subtype: Link Rect: 312, 241, 396, 250 Annotation: Subtype: Link Rect: 372, 212, 460, 222 Annotation: Subtype: Link Rect: 312, 186, 378, 193 Annotation: Subtype: Link Rect: 488, 165, 551, 174 Annotation: Subtype: Link Rect: 312, 157, 375, 165 Annotation: Subtype: Link Rect: 489, 137, 551, 146 Annotation: Subtype: Link Rect: 312, 128, 365, 137 Annotation: Subtype: Link Rect: 470, 81, 551, 89 Annotation: Subtype: Link Rect: 312, 72, 332, 79 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 218, 678, 293, 687 Annotation: Subtype: Link Rect: 53, 670, 75, 677 Annotation: Subtype: Link Rect: 233, 640, 293, 649 Annotation: Subtype: Link Rect: 53, 631, 122, 640 Annotation: Subtype: Link Rect: 196, 593, 293, 602 Annotation: Subtype: Link Rect: 53, 585, 85, 593 Annotation: Subtype: Link Rect: 209, 557, 293, 564 Annotation: Subtype: Link Rect: 53, 547, 73, 554 Annotation: Subtype: Link Rect: 210, 517, 293, 526 Annotation: Subtype: Link Rect: 53, 509, 75, 516 Annotation: Subtype: Link Rect: 239, 470, 293, 479 Annotation: Subtype: Link Rect: 53, 462, 91, 469 Annotation: Subtype: Link Rect: 232, 422, 293, 432 Annotation: Subtype: Link Rect: 53, 415, 91, 422 Annotation: Subtype: Link Rect: 232, 375, 293, 384 Annotation: Subtype: Link Rect: 53, 367, 70, 374 Annotation: Subtype: Link Rect: 73, 339, 170, 347 Annotation: Subtype: Link Rect: 249, 309, 293, 318 Annotation: Subtype: Link Rect: 53, 301, 90, 309 Annotation: Subtype: Link Rect: 231, 252, 293, 261 Annotation: Subtype: Link Rect: 53, 244, 91, 251 Annotation: Subtype: Link Rect: 193, 224, 293, 233 Annotation: Subtype: Link Rect: 53, 216, 74, 223 Annotation: Subtype: Link Rect: 189, 197, 267, 205 Annotation: Subtype: Link Rect: 131, 176, 223, 186 Annotation: Subtype: Link Rect: 139, 158, 202, 167 Annotation: Subtype: Link Rect: 218, 138, 293, 148 Annotation: Subtype: Link Rect: 53, 131, 73, 138 Annotation: Subtype: Link Rect: 222, 91, 293, 101 Annotation: Subtype: Link Rect: 53, 84, 75, 90 Annotation: Subtype: Link Rect: 460, 698, 551, 706 Annotation: Subtype: Link Rect: 312, 689, 335, 696 Annotation: Subtype: Link Rect: 457, 659, 551, 668 Annotation: Subtype: Link Rect: 312, 651, 337, 658 Annotation: Subtype: Link Rect: 484, 631, 551, 640 Annotation: Subtype: Link Rect: 312, 623, 328, 630 Annotation: Subtype: Link Rect: 494, 583, 551, 593 Annotation: Subtype: Link Rect: 312, 576, 328, 583 Annotation: Subtype: Link Rect: 341, 536, 437, 545 Annotation: Subtype: Link Rect: 474, 498, 551, 508 Annotation: Subtype: Link Rect: 312, 491, 334, 497 Annotation: Subtype: Link Rect: 501, 460, 551, 470 Annotation: Subtype: Link Rect: 312, 453, 335, 460 Annotation: Subtype: Link Rect: 473, 432, 551, 441 Annotation: Subtype: Link Rect: 312, 424, 353, 431 Annotation: Subtype: Link Rect: 449, 356, 551, 366 Annotation: Subtype: Link Rect: 312, 349, 328, 355 Annotation: Subtype: Link Rect: 504, 309, 551, 318 Annotation: Subtype: Link Rect: 312, 300, 387, 309 Annotation: Subtype: Link Rect: 452, 262, 551, 271 Annotation: Subtype: Link Rect: 312, 254, 334, 261 Annotation: Subtype: Link Rect: 472, 214, 551, 224 Annotation: Subtype: Link Rect: 312, 207, 328, 213 Annotation: Subtype: Link Rect: 355, 176, 446, 186 Annotation: Subtype: Link Rect: 537, 120, 551, 129 Annotation: Subtype: Link Rect: 312, 110, 397, 119 Annotation: Subtype: Link Rect: 468, 91, 551, 101 Annotation: Subtype: Link Rect: 312, 84, 334, 90 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 209, 659, 293, 668 Annotation: Subtype: Link Rect: 53, 651, 75, 658 Annotation: Subtype: Link Rect: 97, 631, 192, 640 Annotation: Subtype: Link Rect: 137, 612, 246, 621 Annotation: Subtype: Link Rect: 82, 583, 177, 593 Annotation: Subtype: Link Rect: 312, 697, 400, 706 Annotation: Subtype: Link Rect: 323, 621, 349, 631 Annotation: Subtype: Link Rect: 350, 621, 382, 631 Annotation: Subtype: Link Rect: 384, 621, 419, 631 Annotation: Subtype: Link Rect: 420, 621, 443, 631 Annotation: Subtype: Link Rect: 445, 621, 473, 631 Annotation: Subtype: Link Rect: 43, 29, 113, 38 Annotation: Subtype: Link Rect: 116, 29, 176, 38 Annotation: Subtype: Link Rect: 442, 29, 551, 38 Checksum: bdb5feb2 Type: CRC32 Checksum: 0adfc510e7ac32e4a3318371efefb290 Type: MD5 Checksum: d4894a3174a70c7dd20ce6c3f440710e97ec649d Type: SHA-1