c2e7611174fec8e39ff2ec88a0c59efa pmc_31275278.pdf 0a0d20b039b67a5d73fb3617381277c933cb305f pmc_31275278.pdf 8144fad5eb0e6b184db10cfe5f3259f5f3a51416ceee796698ec2e9bf4cf036d pmc_31275278.pdf Title: Testing a Human Antimicrobial RNase Chimera Against Bacterial Resistance Subject: The emergence of bacterial resistance to the most commonly used antibiotics encourages the design of novel antimicrobial drugs. Keywords: RNase, antimicrobial resistance, antibiotic adjuvant, gram-negative bacteria, antimicrobial peptides Author: Ester Boix Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Tue Jun 18 12:30:59 2019 CEST ModDate: Tue Jun 18 12:30:59 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 2716154 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LALJJS+MinionPro-Regular Type 1 Custom yes yes no 39 0 LAMECP+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 40 0 JEBMYB+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 41 0 NRTAUV+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 46 0 AURBTD+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 47 0 AWEQPH+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 48 0 OCMQPJ+MTSYN Type 1 Builtin yes yes no 152 0 PRCSFS+MinionPro-It Type 1 Custom yes yes no 155 0 QHWLRN+MTGU Type 1 Builtin yes yes no 217 0 WOPSSR+RBLMI Type 1 Builtin yes yes no 338 0 CIAIIM+MinionPro-Bold Type 1 Custom yes yes no 346 0 BJPRMX+MTGUB Type 1 Builtin yes yes no 397 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:38:32 CEST RepresentationInformation: pmc_31275278.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-06 04:10:06 CEST Size: 2716154 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 961 FreeObjects: 28 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Testing a Human Antimicrobial RNase Chimera Against Bacterial Resistance Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Materials Item: Title: Protein Expression and Purification Item: Title: Circular Dichroism (CD) Item: Title: Activity Staining Gel Item: Title: Minimum Bactericidal Concentration (MBC) Determination Item: Title: Cytotoxicity Assay Item: Title: LPS Binding Assay Item: Title: Liposome Preparation Item: Title: Liposome Leakage Item: Title: Synergy Determination Assay Item: Title: Antimicrobial Resistance Evolution Assay by Colistin Exposure Item: Title: Minimum Inhibition Concentration (MIC) Determination Item: Title: Statistical Analysis Item: Title: Results Children: Item: Title: RNase 3/1 Rational Design Item: Title: RNase 3/1 Shows Synergy Activity With Colistin Against A. baumannii Item: Title: Exposure of Bacterial Culture to RNase 3/1 Reduces the Acquisition of Bacterial Resistance to Colistin Item: Title: Discussion Item: Title: Conclusion Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: Testing a Human Antimicrobial RNase Chimera Against Bacterial Resistance Author: Ester Boix Subject: The emergence of bacterial resistance to the most commonly used antibiotics encourages the design of novel antimicrobial drugs. 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