2a5533696bc7013dc959008a15488718 pmc_31316491.pdf 4be32fa5a034253d4f345fb7ce53dfb6efc8d233 pmc_31316491.pdf a75f8d076ffc1e57eac6fc7566358f5870f7a48a43ce8e5332e9c77377b2b9cf pmc_31316491.pdf Title: Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris Subject: Antimicrobial peptides (AMPs) have potent and durable antimicrobial activity to a wide range of fungi and bacteria. Keywords: antimicrobial peptides, antifungal peptides, Pichia pastoris, plant oleosin, cecropin A, PAF peptides, lipid droplets Author: María Coca Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed Jun 26 12:14:33 2019 CEST ModDate: Wed Jun 26 12:14:33 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 5588127 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LKZXXL+MinionPro-Regular Type 1 Custom yes yes no 29 0 YSZANT+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 30 0 WINLSQ+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 31 0 BQESRV+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 36 0 HLFAAY+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 37 0 SVDUHO+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 38 0 FFIBFB+MinionPro-It Type 1 Custom yes yes no 165 0 KDBHNV+MinionPro-Bold Type 1 Custom yes yes no 175 0 JYDOCF+MTGU Type 1 Builtin yes yes no 238 0 PBIRRK+MTSYN Type 1 Builtin yes yes no 240 0 PAARCL+RBLMI Type 1 Builtin yes yes no 429 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-11-19 06:12:09 CET RepresentationInformation: pmc_31316491.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-08-03 03:41:19 CEST Size: 5588127 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 831 FreeObjects: 6 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Plasmid Constructs, Yeast Strains and Growth Conditions Item: Title: Lipid Droplets Fractionation Item: Title: Immunoblot Analysis of LD-Associated Proteins and PAF102 Purification Item: Title: Confocal and Fluorescence Microscopy Item: Title: Antifungal Activity Assays Item: Title: Results Children: Item: Title: Ole18-GFP Fusion Protein Is Targeted to P. pastoris LDs Item: Title: Ole18-PAF102 Accumulates in P. pastoris LDs Item: Title: Ole18-CecA Is Susceptible to P. pastoris Proteases Item: Title: Ole18-PAF102 Production Induces Proliferation of LDs in P. pastoris Item: Title: A. thaliana Dgat1 Expression Induces LD Formation in Ole18-PAF102 Yeast Cells Item: Title: Biologically Active PAF102 Is Recovered From Yeast Cells Item: Title: Discussion Item: Title: Data Availability Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris Author: María Coca Subject: Antimicrobial peptides (AMPs) have potent and durable antimicrobial activity to a wide range of fungi and bacteria. Keywords: antimicrobial peptides, antifungal peptides, Pichia pastoris, plant oleosin, cecropin A, PAF peptides, lipid droplets Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed Jun 26 12:44:33 CEST 2019 ModDate: Wed Jun 26 12:44:33 CEST 2019 ID: 0x94e8d8ac78814a47fcfc94f587f38fe5, 0x94e8d8ac78814a47fcfc94f587f38fe5 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: PBIRRK+MTSYN FontSubset: true FirstChar: 2 LastChar: 14 FontDescriptor: FontName: PBIRRK+MTSYN Flags: Symbolic FontBBox: 0, -954, 1043, 900 FontFile: true Font: BaseFont: BQESRV+HelveticaNeueLTStd-BdIt FontSubset: true FirstChar: 42 LastChar: 121 FontDescriptor: FontName: BQESRV+HelveticaNeueLTStd-BdIt Flags: Symbolic FontBBox: -166, -218, 1129, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HLFAAY+HelveticaNeueLTStd-LtIt FontSubset: true FirstChar: 40 LastChar: 243 FontDescriptor: FontName: HLFAAY+HelveticaNeueLTStd-LtIt Flags: Symbolic FontBBox: -165, -214, 1099, 951 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: FFIBFB+MinionPro-It FontSubset: true FirstChar: 3 LastChar: 169 FontDescriptor: FontName: FFIBFB+MinionPro-It Flags: Symbolic FontBBox: -201, -360, 1684, 919 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: SVDUHO+HelveticaNeueLTStd-MdIt FontSubset: true FirstChar: 42 LastChar: 237 FontDescriptor: FontName: SVDUHO+HelveticaNeueLTStd-MdIt Flags: Symbolic FontBBox: -165, -218, 1125, 947 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: LKZXXL+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: LKZXXL+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: PAARCL+RBLMI FontSubset: true FirstChar: 60 LastChar: 60 FontDescriptor: FontName: PAARCL+RBLMI Flags: Symbolic FontBBox: -11, -230, 987, 750 FontFile: true Font: BaseFont: YSZANT+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: YSZANT+HelveticaNeueLTStd-Bd Flags: Symbolic FontBBox: -166, -218, 1078, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: JYDOCF+MTGU FontSubset: true FirstChar: 109 LastChar: 109 FontDescriptor: FontName: JYDOCF+MTGU Flags: Symbolic FontBBox: 25, -213, 839, 683 FontFile: true Font: BaseFont: WINLSQ+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 2 LastChar: 150 FontDescriptor: FontName: WINLSQ+HelveticaNeueLTStd-Lt Flags: Symbolic FontBBox: -166, -214, 1050, 967 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: KDBHNV+MinionPro-Bold FontSubset: true FirstChar: 3 LastChar: 121 FontDescriptor: FontName: KDBHNV+MinionPro-Bold Flags: Symbolic FontBBox: -318, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true XMP: antimicrobial peptides, antifungal peptides, Pichia pastoris, plant oleosin, cecropin A, PAF peptides, lipid droplets pdfTeX-1.40.15 1.5 application/pdf Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris Antimicrobial peptides (AMPs) have potent and durable antimicrobial activity to a wide range of fungi and bacteria. 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