6002bfdb85c8e64927753278cf101e00 pmc_31344882.pdf 9202cd2ef5ebdc370e95f018dccd5996364e8750 pmc_31344882.pdf 83a33fe50daf5d8a1bf1e181fadab462b5be7f0c73dce6db9ab504296a0b4a28 pmc_31344882.pdf Title: In Vitro Cytotoxicity of Oleanolic/Ursolic Acids-Loaded in PLGA Nanoparticles in Different Cell Lines Subject: Oleanolic (OA) and ursolic (UA) acids are recognized triterpenoids with anti-cancer properties, showing cell-specific activity that can be enhanced when loaded into polymeric nanoparticles. The cytotoxic activity of OA and UA was assessed by Alamar Blue assay in three different cell lines, i.e., HepG2 (Human hepatoma cell line), Caco-2 (Human epithelial colorectal adenocarcinoma cell line) and Y-79 (Human retinoblastoma cell line). The natural and synthetic mixtures of these compounds were tested as free and loaded in polymeric nanoparticles in a concentration range from 2 to 32 mol/L. The highest tested concentrations of the free triterpene mixtures produced statistically significant cell viability reduction in HepG2 and Caco-2 cells, compared to the control (untreated cells). When loaded in the developed PLGA nanoparticles, no differences were recorded for the tested concentrations in the same cell lines. However, in the Y-79 cell line, a decrease on cell viability was observed when testing the lowest concentration of both free triterpene mixtures, and after their loading into PLGA nanoparticles. Keywords: oleanolic acid; ursolic acid; cytotoxicity; PLGA; polymeric nanoparticles; retinoblastoma cell line Author: Amélia M. Silva, Helen L. Alvarado, Guadalupe Abrego, Carlos Martins-Gomes, Maria L. Garduño-Ramirez, María L. García, Ana C. Calpena and Eliana B. 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IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぃとづねどっちぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぐひはつふっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちぴふひちぬぜ〴ぜ〰ぜ〵ぜ〰ぎきぜ〵ㅜ〰ぜ〴ぜ〰ちのつぜ〴ぜ〰こべのぴとづぴどっぜ〴ぜ〰ぜ〵ぜ〰こきぜ〵ㅜ〰ぜ〴ぜ〰きどへぴふひづびぜ〴ぜ〰はてぜ〴ぜ〰ぐづのぴちっべっぬどっぜ〴ぜ〰ごひどぴづひばづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちのはばちひぴどっぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぃとづねどっちぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぐひはつふっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちぴふひちぬぜ〴ぜ〰ぜ〵ぜ〰ぎきぜ〵ㅜ〰ぜ〴ぜ〰ちのつぜ〴ぜ〰こべのぴとづぴどっぜ〴ぜ〰ぜ〵ぜ〰こきぜ〵ㅜ〰ぜ〴ぜ〰きどへぴふひづびぜ〴ぜ〰はてぜ〴ぜ〰ぐづのぴちっべっぬどっぜ〴ぜ〰ごひどぴづひばづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちのはばちひぴどっぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぐひはつふっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちぴふひちぬぜ〴ぜ〰ぜ〵ぜ〰ぎきぜ〵ㅜ〰ぜ〴ぜ〰ちのつぜ〴ぜ〰こべのぴとづぴどっぜ〴ぜ〰ぜ〵ぜ〰こきぜ〵ㅜ〰ぜ〴ぜ〰きどへぴふひづびぜ〴ぜ〰はてぜ〴ぜ〰ぐづのぴちっべっぬどっぜ〴ぜ〰ごひどぴづひばづのづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちのはばちひぴどっぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちのはばちひぴどっぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃづぬぬぜ〴ぜ〰ぃふぬぴふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴ぜ〰ちのつぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぃとちひちっぴづひどぺちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちのはばちひぴどっぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ぃとちひちっぴづひどぺちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぎちのはばちひぴどっぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃべぴはぴはへどっどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: THGEKM+Rpxsc FontSubset: true FirstChar: 100 LastChar: 108 FontDescriptor: FontName: THGEKM+Rpxsc Flags: Symbolic FontBBox: -37, -172, 852, 718 FontFile: true Font: BaseFont: UNQNNY+Pxsy FontSubset: true FirstChar: 0 LastChar: 21 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