e8eddb35a34073b2c506c489f7f9c451 pmc_31387236.pdf 6346ed4567c08bb63485abd7ca86f5a0a65b5925 pmc_31387236.pdf ca6046e661c6c5fb3f37b5fdf7310e3b88427cc66eeeb4c8ba913eeecf494860 pmc_31387236.pdf Title: Protein Phosphatase Ppz1 Is Not Regulated by a Hal3-Like Protein in Plant Pathogen Ustilago maydis Subject: Ppz enzymes are type-1 related Ser/Thr protein phosphatases that are restricted to fungi. In S. cerevisiae and other fungi, Ppz1 is involved in cation homeostasis and is regulated by two structurally-related inhibitory subunits, Hal3 and Vhs3, with Hal3 being the most physiologically relevant. Remarkably, Hal3 and Vhs3 have moonlighting properties, as they participate in an atypical heterotrimeric phosphopantothenoyl cysteine decarboxylase (PPCDC), a key enzyme for Coenzyme A biosynthesis. Here we identify and functionally characterize Ppz1 phosphatase (UmPpz1) and its presumed regulatory subunit (UmHal3) in the plant pathogen fungus Ustilago maydis. UmPpz1 is not an essential protein in U. maydis and, although possibly related to the cell wall integrity pathway, is not involved in monovalent cation homeostasis. The expression of UmPpz1 in S. cerevisiae Ppz1-deficient cells partially mimics the functions of the endogenous enzyme. In contrast to what was found in C. albicans and A. fumigatus, UmPpz1 is not a virulence determinant. UmHal3, an unusually large protein, is the only functional PPCDC in U. maydis and, therefore, an essential protein. However, when overexpressed in U. maydis or S. cerevisiae, UmHal3 does not reproduce Ppz1-inhibitory phenotypes. Indeed, UmHal3 does not inhibit UmPpz1 in vitro (although ScHal3 does). Therefore, UmHal3 might not be a moonlighting protein. Keywords: protein phosphorylation; protein phosphatase; CoA biosynthesis; Ustilago maydis; fungi Author: Chunyi Zhang, Antonio de la Torre, José Pérez-Martín and Joaquín Ariño Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Mon Aug 5 09:55:16 2019 CEST ModDate: Mon Aug 5 09:55:16 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4161864 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HIABTM+URWPalladioL-Ital Type 1 Custom yes yes no 122 0 DSTHUQ+URWPalladioL-Bold Type 1 Custom yes yes no 123 0 ZLSXRX+URWPalladioL-BoldItal Type 1 Custom yes yes no 124 0 YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 125 0 KFUNZT+URWPalladioL-Roma Type 1 Custom yes yes no 126 0 AHRQMW+VnURWPalladioL Type 1 Custom yes yes no 127 0 QANVCD+Rpxr Type 1 Builtin yes yes no 128 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 133 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 134 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 153 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 154 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 155 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 216 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 217 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 218 0 Times New Roman TrueType WinAnsi no no no 219 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 255 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 258 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 259 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 260 0 Times New Roman TrueType WinAnsi no no no 261 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 290 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 291 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 292 0 Times New Roman TrueType WinAnsi no no no 293 0 HZWWXG+Pxsy Type 1 Builtin yes yes no 316 0 EUIVHG+Pxsyc Type 1 Builtin yes yes no 317 0 VJOJFS+Pxsya Type 1 Builtin yes yes no 318 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 322 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 323 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 324 0 Times New Roman TrueType WinAnsi no no no 325 0 ABCDEE+Wingdings 2 CID TrueType Identity-H yes yes yes 326 0 EBLKOD+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 351 0 EBLKOF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 352 0 EBLKOH+PalatinoLinotype TrueType WinAnsi yes yes no 353 0 EBLKPH+TimesNewRoman CID TrueType Identity-H yes yes yes 354 0 EBLLBH+Wingdings2 CID TrueType Identity-H yes yes yes 355 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 389 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 390 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 391 0 Times New Roman TrueType WinAnsi no no no 392 0 Symbol CID TrueType Identity-H yes no yes 393 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 424 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 427 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 428 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 429 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 430 0 Times New Roman TrueType WinAnsi no no no 431 0 Symbol CID TrueType Identity-H yes no yes 432 0 ABCDEE+Palatino Linotype,Italic CID TrueType Identity-H yes yes yes 433 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 481 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 482 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 483 0 Times New Roman TrueType WinAnsi no no no 484 0 ABCDEE+Palatino Linotype,Italic CID TrueType Identity-H yes yes yes 485 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 506 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 507 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 508 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 509 0 Times New Roman TrueType WinAnsi no no no 510 0 ABCDEE+Palatino Linotype,Italic CID TrueType Identity-H yes yes yes 511 0 ABCDEE+Palatino Linotype TrueType WinAnsi yes yes no 562 0 ABCDEE+Palatino Linotype,Italic TrueType WinAnsi yes yes no 563 0 ABCDEE+Palatino Linotype,Bold TrueType WinAnsi yes yes no 564 0 ABCDEE+Palatino Linotype CID TrueType Identity-H yes yes yes 565 0 Times New Roman TrueType WinAnsi no no no 566 0 ABCDEE+Palatino Linotype,Italic CID TrueType Identity-H yes yes yes 567 0 MFIGJL+Rpxb Type 1 Builtin yes yes no 663 0 YWGLGW+Rpxi Type 1 Builtin yes yes no 664 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 776 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-11-19 06:11:19 CET RepresentationInformation: pmc_31387236.pdf ReportingModule: BYTESTREAM, Rel. 1.4 (2018-10-01) LastModified: 2020-08-03 03:40:42 CEST Size: 4161864 Format: bytestream Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/octet-stream Checksum: 3884420e Type: CRC32 Checksum: e8eddb35a34073b2c506c489f7f9c451 Type: MD5 Checksum: 6346ed4567c08bb63485abd7ca86f5a0a65b5925 Type: SHA-1