7b3585a1851a95e2eefbc2f38128f7fd pmc_31554159.pdf ce6097106f2226ea8c1fa8828161b47f11a55e7a pmc_31554159.pdf cb85475357f3f46f469f878182b88db99c450ddccab402b5f07815d7078ad994 pmc_31554159.pdf Title: Microwave-Assisted Synthesis of SPION-Reduced Graphene Oxide Hybrids for Magnetic Resonance Imaging (MRI) Subject: Magnetic resonance imaging (MRI) is a useful tool for disease diagnosis and treatment monitoring. Superparamagnetic iron oxide nanoparticles (SPION) show good performance as transverse relaxation (T2) contrast agents, thus facilitating the interpretation of the acquired images. Attachment of SPION onto nanocarriers prevents their agglomeration, improving the circulation time and efficiency. Graphene derivatives, such as graphene oxide (GO) and reduced graphene oxide (RGO), are appealing nanocarriers since they have both high surface area and functional moieties that make them ideal substrates for the attachment of nanoparticles. We have employed a fast, simple and environmentally friendly microwave-assisted approach for the synthesis of SPION-RGO hybrids. Different iron precursor/GO ratios were used leading to SPION, with a median diameter of 7.1 nm, homogeneously distributed along the RGO surface. Good relaxivity (r2*) values were obtained in MRI studies and no significant toxicity was detected within in vitro tests following GL261 glioma and J774 macrophage-like cells for 24 h with SPION-RGO, demonstrating the applicability of the hybrids as T2-weighted MRI contrast agents. Keywords: contrast agents; biomedical imaging; ultrasmall superparamagnetic iron oxide nanoparticles Author: Marina Llenas, Stefania Sandoval, Pedro M. Costa, Judith Oró-Solé, Silvia Lope-Piedrafita, Belén Ballesteros, Khuloud T. Al-Jamal and Gerard Tobias Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Tue Sep 24 05:29:58 2019 CEST ModDate: Tue Sep 24 05:29:58 2019 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5628529 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HZGKKI+URWPalladioL-Ital Type 1 Custom yes yes no 79 0 PCLBRO+URWPalladioL-Bold Type 1 Custom yes yes no 80 0 HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes no 81 0 LMEOCP+URWPalladioL-Roma Type 1 Custom yes yes no 82 0 XVFHJX+VnURWPalladioL Type 1 Custom yes yes no 83 0 QANVCD+Rpxr Type 1 Builtin yes yes no 84 0 NPLVPL+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 89 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 106 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom 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IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰げづちでづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰こべのぴとづびどびぜ〴ぜ〰はてぜ〴ぜ〰ぇひちばとづのづぜ〴ぜ〰くへどつづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰いづっはひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰げぇくぜ〴ぜ〰ぷどぴとぜ〴ぜ〰こぐぉくぎぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃとちひちっぴづひどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きげぉぜ〴ぜ〰びぴふつどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰こぴちぢどぬどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きはつどてどづつぜ〴ぜ〰がちっぴちぴづぜ〴ぜ〰いづとべつひはでづのちびづぜ〴ぜ〰ぜ〵ぜ〰がいえぜ〵ㅜ〰ぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: げづちでづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰こべのぴとづびどびぜ〴ぜ〰はてぜ〴ぜ〰ぇひちばとづのづぜ〴ぜ〰くへどつづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰いづっはひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰げぇくぜ〴ぜ〰ぷどぴとぜ〴ぜ〰こぐぉくぎぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃとちひちっぴづひどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きげぉぜ〴ぜ〰びぴふつどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰こぴちぢどぬどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きはつどてどづつぜ〴ぜ〰がちっぴちぴづぜ〴ぜ〰いづとべつひはでづのちびづぜ〴ぜ〰ぜ〵ぜ〰がいえぜ〵ㅜ〰ぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こべのぴとづびどびぜ〴ぜ〰はてぜ〴ぜ〰ぇひちばとづのづぜ〴ぜ〰くへどつづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰いづっはひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰げぇくぜ〴ぜ〰ぷどぴとぜ〴ぜ〰こぐぉくぎぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃとちひちっぴづひどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きげぉぜ〴ぜ〰びぴふつどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰こぴちぢどぬどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きはつどてどづつぜ〴ぜ〰がちっぴちぴづぜ〴ぜ〰いづとべつひはでづのちびづぜ〴ぜ〰ぜ〵ぜ〰がいえぜ〵ㅜ〰ぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: いづっはひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰げぇくぜ〴ぜ〰ぷどぴとぜ〴ぜ〰こぐぉくぎぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぃとちひちっぴづひどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きげぉぜ〴ぜ〰びぴふつどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰こぴちぢどぬどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きはつどてどづつぜ〴ぜ〰がちっぴちぴづぜ〴ぜ〰いづとべつひはでづのちびづぜ〴ぜ〰ぜ〵ぜ〰がいえぜ〵ㅜ〰ぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぃとちひちっぴづひどぺちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きげぉぜ〴ぜ〰びぴふつどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰こぴちぢどぬどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きはつどてどづつぜ〴ぜ〰がちっぴちぴづぜ〴ぜ〰いづとべつひはでづのちびづぜ〴ぜ〰ぜ〵ぜ〰がいえぜ〵ㅜ〰ぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きげぉぜ〴ぜ〰びぴふつどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰こぴちぢどぬどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きはつどてどづつぜ〴ぜ〰がちっぴちぴづぜ〴ぜ〰いづとべつひはでづのちびづぜ〴ぜ〰ぜ〵ぜ〰がいえぜ〵ㅜ〰ぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰こぴちぢどぬどぴべぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ぉのぜ〴ぜ〰ざどぴひはぜ〴ぜ〰きはつどてどづつぜ〴ぜ〰がちっぴちぴづぜ〴ぜ〰いづとべつひはでづのちびづぜ〴ぜ〰ぜ〵ぜ〰がいえぜ〵ㅜ〰ぜ〴ぜ〰ぁびびちべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: 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