e43e4c9982a1fddc2a62942c858c045f pmc_31649540.pdf 2a0fc629e5d15705eccd1c00424ac85006666abf pmc_31649540.pdf 3f004e7e559265078c46813a3f3e74dc8b39c9b4ae3473a1e62b3d085caf2612 pmc_31649540.pdf Title: Evolutionary Trends in RNA Base Selectivity Within the RNase A Superfamily Subject: There is a growing interest in the pharmaceutical industry to design novel tailored drugs for RNA targeting. Keywords: RNase, RNA, purine, catalysis, molecular dynamics, evolution, RNase A superfamily Author: Guillem Prats-Ejarque Creator: Adobe InDesign CC 2015 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Tue Oct 8 06:41:49 2019 CEST ModDate: Tue Oct 8 07:20:41 2019 CEST Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 3491088 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HelveticaNeueLTStd-Bd Type 1C Custom yes no yes 2120 0 HelveticaNeueLTStd-Lt Type 1C Custom yes no yes 2121 0 HelveticaNeueLTStd-MdIt Type 1C WinAnsi yes no yes 2133 0 HelveticaNeueLTStd-LtIt Type 1C Custom yes no yes 2134 0 KUCCXE+STIXGeneral Type 1C Custom yes yes yes 2140 0 HelveticaNeueLTStd-BdIt Type 1C WinAnsi yes no yes 2145 0 MinionPro-Regular Type 1C Custom yes no yes 226 0 MinionPro-It Type 1C Custom yes no yes 221 0 MinionPro-Bold Type 1C Custom yes no yes 269 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:23:15 CEST RepresentationInformation: pmc_31649540.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-06 04:07:39 CEST Size: 3491088 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 2193 FreeObjects: 1721 IncrementalUpdates: 3 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Evolutionary Trends in RNA Base Selectivity Within the RNase A Superfamily Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Expression and Purification of the Recombinant Proteins Item: Title: Spectrophotometric Kinetic Analysis Item: Title: Molecular Dynamics Simulations Item: Title: Results Children: Item: Title: Comparison of Canonical RNases’ Catalytic Activity on Dinucleotide Substrates: A Trend From Guanine to Adenine Selectivity At the B2 Secondary Base Site Item: Title: B2 Base Selectivity Within the RNase A Superfamily by Molecular Dynamics Item: Title: An Evolutionary Trend Shaping the B2 Selectivity Within the RNase A Superfamily Lineage Item: Title: Discussion Item: Title: Conclusions Item: Title: Data Availability Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: Evolutionary Trends in RNA Base Selectivity Within the RNase A Superfamily Author: Guillem Prats-Ejarque Subject: There is a growing interest in the pharmaceutical industry to design novel tailored drugs for RNA targeting. Keywords: RNase, RNA, purine, catalysis, molecular dynamics, evolution, RNase A superfamily Creator: Adobe InDesign CC 2015 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Tue Oct 08 06:41:49 CEST 2019 ModDate: Tue Oct 08 07:20:41 CEST 2019 ID: 0xedafdd9477b13c4d8a3ac4ae272bc72c, 0xd695402fecc2b44ebd3d7fe72cd04062 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2063 ImageHeight: 944 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1536 ImageHeight: 968 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1772 ImageHeight: 997 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1772 ImageHeight: 1218 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 976 ImageHeight: 527 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 976 ImageHeight: 415 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Fonts: Type1: Font: BaseFont: HelveticaNeueLTStd-BdIt FirstChar: 0 LastChar: 255 FontDescriptor: FontName: HelveticaNeueLTStd-BdIt Flags: Nonsymbolic, Italic FontBBox: -166, -218, 1129, 975 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-Regular FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Regular Flags: Serif, Nonsymbolic FontBBox: -290, -360, 1684, 989 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: HelveticaNeueLTStd-MdIt FirstChar: 0 LastChar: 255 FontDescriptor: FontName: HelveticaNeueLTStd-MdIt Flags: Nonsymbolic, Italic FontBBox: -165, -218, 1125, 947 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: HelveticaNeueLTStd-LtIt FirstChar: 0 LastChar: 255 FontDescriptor: FontName: HelveticaNeueLTStd-LtIt Flags: Nonsymbolic, Italic FontBBox: -165, -214, 1099, 951 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: HelveticaNeueLTStd-Bd FirstChar: 0 LastChar: 255 FontDescriptor: FontName: HelveticaNeueLTStd-Bd Flags: Nonsymbolic FontBBox: -166, -218, 1078, 975 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: HelveticaNeueLTStd-Lt FirstChar: 0 LastChar: 255 FontDescriptor: FontName: HelveticaNeueLTStd-Lt Flags: Nonsymbolic FontBBox: -166, -214, 1050, 967 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: KUCCXE+STIXGeneral FontSubset: true FirstChar: 29 LastChar: 98 FontDescriptor: FontName: KUCCXE+STIXGeneral Flags: Serif, Nonsymbolic FontBBox: -178, -443, 2000, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MinionPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-It Flags: Serif, Nonsymbolic, Italic FontBBox: -201, -360, 1684, 1002 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MinionPro-Bold FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Bold Flags: Serif, Nonsymbolic FontBBox: -319, -360, 1684, 1024 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true XMP: application/pdf Evolutionary Trends in RNA Base Selectivity Within the RNase A Superfamily There is a growing interest in the pharmaceutical industry to design novel tailored drugs for RNA targeting. Guillem Prats-Ejarque Lu Lu Vivian A. Salazar Mohammed Moussaoui Ester Boix RNase RNA purine catalysis molecular dynamics evolution RNase A superfamily 2019-10-08T12:41:49+08:00 2019-10-08T13:20:41+08:00 2019-10-08T13:20:41+08:00 Adobe InDesign CC 2015 (Windows) uuid:5024cc33-0fca-4c2d-ba6a-83094ed86ba5 xmp.did:2aecd8bd-cbd3-4539-b083-9de383973cbe xmp.id:4b2d0997-ce1b-c14a-b49e-00a2b4b23f23 proof:pdf xmp.iid:cc0536ed-ba6d-5440-8115-107b34619571 xmp.did:c5b2faf7-74d8-fc4f-8159-2a338f8fe2f3 xmp.did:2aecd8bd-cbd3-4539-b083-9de383973cbe default converted from application/x-indesign to application/pdf Adobe InDesign CC 2015 (Windows) / 2019-10-08T12:41:49+08:00 Adobe PDF Library 15.0 False RNase, RNA, purine, catalysis, molecular dynamics, evolution, RNase A superfamily Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 419, 39, 551, 30 Annotation: Subtype: Link Rect: 453, 745, 551, 736 Annotation: Subtype: Link Rect: 468, 728, 549, 720 Annotation: Subtype: Link Rect: 453, 737, 551, 728 Annotation: Subtype: 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