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Title:          Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Subject:        Microbial Cell Factories, https://doi.org/10.1186/s12934-019-1240-8
Keywords:       AOX1 promoter,Heterologous gene dosage,Transcription analysis,Pichia pastoris,Gene expression/regulation,MIT1,Specific growth rate influence,Operational mode
Author:         Javier Garrigós-Martínez 
Creator:        Springer
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     Item: 
      Title: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
      Children: 
       Item: 
        Title: Abstract 
        Children: 
         Item: 
          Title: Background: 
         Item: 
          Title: Results: 
         Item: 
          Title: Conclusions: 
       Item: 
        Title: Background
       Item: 
        Title: Results and discussion
        Children: 
         Item: 
          Title: Effect of increasing CRL1 gene dosage on culture physiological state
         Item: 
          Title: Relationship between Mit1 limitation and a decreased AOX1 relative expression (RE)
         Item: 
          Title: Influence of operational mode on production-related parameters
         Item: 
          Title: Increasing CRL1 gene dosage boosts protein production
         Item: 
          Title: Transcriptional efficiency differences between AOX1 and CRL1 genes, and their impact on Crl1 production
       Item: 
        Title: Conclusions
       Item: 
        Title: Materials and methods
        Children: 
         Item: 
          Title: Plasmid construction and strain generation
         Item: 
          Title: Gene dosage determination
         Item: 
          Title: Total RNA extraction
         Item: 
          Title: Synthesis of cDNA and determination of transcriptional levels
         Item: 
          Title: Chemostat cultivation
         Item: 
          Title: Fed-batch cultivation
         Item: 
          Title: Biomass determination as dry cell weight (DCW)
         Item: 
          Title: Quantification of the carbon source and byproducts
         Item: 
          Title: Off-gas analyses
         Item: 
          Title: Lipolytic activity
         Item: 
          Title: Process parameters determination, consistency checking and data reconciliation
          Children: 
           Item: 
            Title: Mass balance and stoichiometric equations
           Item: 
            Title: Consistency checking and data reconciliation
       Item: 
        Title: Acknowledgements
       Item: 
        Title: References
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    Title: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
    Author: Javier Garrigós-Martínez 
    Subject: Microbial Cell Factories, https://doi.org/10.1186/s12934-019-1240-8
    Keywords: AOX1 promoter,Heterologous gene dosage,Transcription analysis,Pichia pastoris,Gene expression/regulation,MIT1,Specific growth rate influence,Operational mode
    Creator: Springer
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