3052bb039e42131c0462091f4dc5a863 pmc_31684085.pdf f7e1f3bdbf7c08f6623d8c3070bf5237fd469a5a pmc_31684085.pdf f83f972cbc565a2eda35e2a02c10bc676e59b30e5bb9662dd018101fa3340159 pmc_31684085.pdf Title: Evaluation of Continuous UVC Treatments and its Combination with UHPH on Spores of Bacillus subtilis in Whole and Skim Milk Subject: The aim of this study was to evaluate the effectiveness of different UVC treatments, alone or in combination with ultra-high pressure homogenization (UHPH) on Bacillus subtilis spores in milk. Spores of B. subtilis (CECT4002) were inoculated in whole and skim milk to an initial concentration about 6 log CFU/mL. Milk was subjected to different ultraviolet radiation treatments at 254 nm (UVC) using a concentric tubular reactor in a dose ranging from 10 to 160 J/mL. Different number of passes were used to adjust the final dose received by the matrix. In general, increasing the number of passes (defined as number of entries to the tunnel-NET) increased the inactivation of spores of B. subtilis. The best lethality results (above 4 Log CFU/mL) were obtained by applying doses from 100 J/mL with several NET. When the same doses were achieved with a single pass lethality in most cases did not exceed 1 log CFU/mL. Increasing the NET also increased the likelihood for the spores to remain longer in the effective distance from the UVC source, estimated as 0.02 mm for whole milk and 0.06 mm for skim milk. Combination of UHPH and UVC did not clearly increase the efficiency of a single UVC treatment, and a lower lethality was even observed in some cases. UHPH treatments increased the turbidity and absorption coefficient (254 nm) of both whole and skim milk. Keywords: UVC; UHPH; milk; Bacillus subtilis Author: María Martinez-Garcia, Jezer N. Sauceda-Gálvez, Idoia Codina-Torrella, Mª Manuela Hernández-Herrero, Ramón Gervilla and Artur X. Roig-Sagués Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Sat Nov 2 10:48:55 2019 CET ModDate: Sat Nov 2 10:48:55 2019 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1931758 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XMRRDM+URWPalladioL-Ital Type 1 Custom yes yes no 135 0 HRUAWX+URWPalladioL-Bold Type 1 Custom yes yes no 136 0 CMKMIC+URWPalladioL-BoldItal Type 1 Custom yes yes no 137 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 138 0 YKIOEA+URWPalladioL-Roma Type 1 Custom yes yes no 139 0 OHKNFJ+VnURWPalladioL Type 1 Custom yes yes no 140 0 FATHTC+Rpxr Type 1 Builtin yes yes no 141 0 XBNVAE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 146 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 163 0 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LONAKE+PalatinoLinotype CID TrueType Identity-H yes yes yes 324 0 LONAKF+PalatinoLinotype TrueType WinAnsi yes yes no 325 0 LONBAE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 326 0 LONBBG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 327 0 LONAKF+PalatinoLinotype TrueType WinAnsi yes yes no 358 0 LONBAE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 359 0 LONBBG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 360 0 LONFCB+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 374 0 LONAKF+PalatinoLinotype TrueType WinAnsi yes yes no 375 0 LONBAE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 376 0 LONBBG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 377 0 MTRAWD+Pxsya Type 1 Builtin yes yes no 414 0 LONAKE+PalatinoLinotype CID TrueType Identity-H yes yes yes 419 0 LONAKF+PalatinoLinotype TrueType WinAnsi yes yes no 420 0 LONBAE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 421 0 LONBBG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 422 0 LONAKE+PalatinoLinotype CID TrueType Identity-H yes yes yes 452 0 LONAKF+PalatinoLinotype TrueType WinAnsi yes yes no 453 0 LONBAE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 454 0 LONBBG+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 455 0 NUUXUW+VnURWPalladioL-Italic Type 1 Custom yes yes no 577 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-11-19 06:08:40 CET RepresentationInformation: pmc_31684085.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-08-17 03:37:04 CEST Size: 1931758 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 5 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こばはひづぜ〴ぜ〰こふびばづのびどはのぜ〴ぜ〰はてぜ〴ぜ〰あ〮ぜ〴ぜ〰こふぢぴどぬどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ごべばづびぜ〴ぜ〰はてぜ〴ぜ〰きどぬにぜ〴ぜ〰さびづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸲⸱ Children: Item: Title: ぐひづばちひちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こばはひづぜ〴ぜ〰こふびばづのびどはのぜ〴ぜ〰はてぜ〴ぜ〰あ〮ぜ〴ぜ〰こふぢぴどぬどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ごべばづびぜ〴ぜ〰はてぜ〴ぜ〰きどぬにぜ〴ぜ〰さびづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸲⸱ Item: Title: ごべばづびぜ〴ぜ〰はてぜ〴ぜ〰きどぬにぜ〴ぜ〰さびづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸲⸱ Children: Item: Title: ぁぢびはひばぴどはのぜ〴ぜ〰ぃはづててどっどづのぴぜ〴ぜ〰ちぴぜ〴ぜ〰〲〵〴ぜ〴ぜ〰のねぜ〴ぜ〰ぜ〵ぜ〰ぜ〵ぜ〰〲〵〴ぜ〵ㅜ〰ぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸲⸲ Item: Title: ごふひぢどつどぴべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぁばばぬどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰さざぃぜ〴ぜ〰げちつどちぴどはのぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Item: Title: ぁばばぬどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰さざぃぜ〴ぜ〰げちつどちぴどはのぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Children: Item: Title: さざぃぜ〴ぜ〰げづちっぴはひぜ〴ぜ〰うづちぴふひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸲ Item: Title: さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸳ Item: Title: いはびどねづぴひべぜ〴ぜ〰さびどのでぜ〴ぜ〰ちぜ〴ぜ〰ぐはぴちびびどふねぜ〴ぜ〰ぉはつどつづ〯ぉはつちぴづぜ〴ぜ〰ぁっぴどのはねづぴづひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸴ Item: Title: いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ごべばづぜ〴ぜ〰はてぜ〴ぜ〰うぬはぷぜ〴ぜ〰どのぴはぜ〴ぜ〰ぴとづぜ〴ぜ〰げづちっぴはひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸵ Item: Title: いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぅててづっぴどぶづぜ〴ぜ〰いづばぴとぜ〴ぜ〰はてぜ〴ぜ〰さざぃぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰さえぐえぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰きどっひはぢどはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こちねばぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰がづぴとちぬどぴべぜ〴ぜ〰ぁっとどづぶづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰かどのづぴどっびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴぜ〴ぜ〰ざちひどちぢぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰さざぃぜ〴ぜ〰うはふひぜ〴ぜ〰いづっどねちぬぜ〴ぜ〰げづつふっぴどはのぜ〴ぜ〰ざちぬふづぜ〴ぜ〰ぜ〵ぜ〰〴いふぶっぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: さえぐえぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰きどっひはぢどはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こちねばぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰がづぴとちぬどぴべぜ〴ぜ〰ぁっとどづぶづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰かどのづぴどっびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴぜ〴ぜ〰ざちひどちぢぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰さざぃぜ〴ぜ〰うはふひぜ〴ぜ〰いづっどねちぬぜ〴ぜ〰げづつふっぴどはのぜ〴ぜ〰ざちぬふづぜ〴ぜ〰ぜ〵ぜ〰〴いふぶっぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: きどっひはぢどはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰こちねばぬづびぜ〴ぜ〰ちのつぜ〴ぜ〰ぃちぬっふぬちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰がづぴとちぬどぴべぜ〴ぜ〰ぁっとどづぶづつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰かどのづぴどっびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴぜ〴ぜ〰ざちひどちぢぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰さざぃぜ〴ぜ〰うはふひぜ〴ぜ〰いづっどねちぬぜ〴ぜ〰げづつふっぴどはのぜ〴ぜ〰ざちぬふづぜ〴ぜ〰ぜ〵ぜ〰〴いふぶっぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰かどのづぴどっびぜ〴ぜ〰ちのつぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴぜ〴ぜ〰ざちひどちぢぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰さざぃぜ〴ぜ〰うはふひぜ〴ぜ〰いづっどねちぬぜ〴ぜ〰げづつふっぴどはのぜ〴ぜ〰ざちぬふづぜ〴ぜ〰ぜ〵ぜ〰〴いふぶっぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: いづぴづひねどのちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰さざぃぜ〴ぜ〰うはふひぜ〴ぜ〰いづっどねちぬぜ〴ぜ〰げづつふっぴどはのぜ〴ぜ〰ざちぬふづぜ〴ぜ〰ぜ〵ぜ〰〴いふぶっぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰がづぴとちぬぜ〴ぜ〰づててづっぴぜ〴ぜ〰はてぜ〴ぜ〰さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴ぜ〰はのぜ〴ぜ〰あちっどぬぬふびぜ〴ぜ〰こふぢぴどぬどびぜ〴ぜ〰こばはひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰がづぴとちぬぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰さえぐえ〭さざぃぜ〴ぜ〰ぃはねぢどのづつぜ〴ぜ〰ごひづちぴねづのぴびぜ〴ぜ〰はのぜ〴ぜ〰あちっどぬぬふびぜ〴ぜ〰こふぢぴどぬどびぜ〴ぜ〰こばはひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰きちぴとづねちぴどっちぬぜ〴ぜ〰きはつづぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰かどのづぴどっびぜ〴ぜ〰はてぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぅびぴどねちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いふぶっぜ〴ぜ〰ざちぬふづぜ〴ぜ〰てはひぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: がづぴとちぬぜ〴ぜ〰づててづっぴぜ〴ぜ〰はてぜ〴ぜ〰さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴ぜ〰はのぜ〴ぜ〰あちっどぬぬふびぜ〴ぜ〰こふぢぴどぬどびぜ〴ぜ〰こばはひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰がづぴとちぬぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰さえぐえ〭さざぃぜ〴ぜ〰ぃはねぢどのづつぜ〴ぜ〰ごひづちぴねづのぴびぜ〴ぜ〰はのぜ〴ぜ〰あちっどぬぬふびぜ〴ぜ〰こふぢぴどぬどびぜ〴ぜ〰こばはひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰きちぴとづねちぴどっちぬぜ〴ぜ〰きはつづぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰かどのづぴどっびぜ〴ぜ〰はてぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぅびぴどねちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いふぶっぜ〴ぜ〰ざちぬふづぜ〴ぜ〰てはひぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: がづぴとちぬぜ〴ぜ〰ぅててづっぴぜ〴ぜ〰はてぜ〴ぜ〰さえぐえ〭さざぃぜ〴ぜ〰ぃはねぢどのづつぜ〴ぜ〰ごひづちぴねづのぴびぜ〴ぜ〰はのぜ〴ぜ〰あちっどぬぬふびぜ〴ぜ〰こふぢぴどぬどびぜ〴ぜ〰こばはひづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰きちぴとづねちぴどっちぬぜ〴ぜ〰きはつづぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰かどのづぴどっびぜ〴ぜ〰はてぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぅびぴどねちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いふぶっぜ〴ぜ〰ざちぬふづぜ〴ぜ〰てはひぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: きちぴとづねちぴどっちぬぜ〴ぜ〰きはつづぬどのでぜ〴ぜ〰はてぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰かどのづぴどっびぜ〴ぜ〰はてぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぅびぴどねちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いふぶっぜ〴ぜ〰ざちぬふづぜ〴ぜ〰てはひぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: かどのづぴどっびぜ〴ぜ〰はてぜ〴ぜ〰ぉのちっぴどぶちぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぅびぴどねちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰いふぶっぜ〴ぜ〰ざちぬふづぜ〴ぜ〰てはひぜ〴ぜ〰ぴとづぜ〴ぜ〰いどててづひづのぴぜ〴ぜ〰さざぃぜ〴ぜ〰ごひづちぴねづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: NUUXUW+VnURWPalladioL-Italic FontSubset: true FirstChar: 178 LastChar: 178 FontDescriptor: 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