001e1af3277d0bc3c19b880a6d3ea9bf pmc_31723265.pdf 5fca3cc61daaca5d762fcbff1f6f9f7db3a59c6e pmc_31723265.pdf 76192f09e48965ac8ae557c47fe6476b0db391e0770e0cea9e1093127af0e763 pmc_31723265.pdf Title: Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease Author: Yi Duan, Cristina Llorente, Sonja Lang, Katharina Brandl, Huikuan Chu, Lu Jiang, Richard C. White, Thomas H. Clarke, Kevin Nguyen, Manolito Torralba, Yan Shao, Jinyuan Liu, Adriana Hernandez-Morales, Lauren Lessor, Imran R. Rahman, Yukiko Miyamoto, Melissa Ly, Bei Gao, Weizhong Sun, Roman Kiesel, Felix Hutmacher, Suhan Lee, Meritxell Ventura-Cots, Francisco Bosques-Padilla, Elizabeth C. Verna, Juan G. Abraldes, Robert S. Brown, Victor Vargas, Jose Altamirano, Juan Caballería, Debbie L. Shawcross, Samuel B. Ho, Alexandre Louvet, Michael R. Lucey, Philippe Mathurin, Guadalupe Garcia-Tsao, Ramon Bataller, Xin M. Tu, Lars Eckmann, Wilfred A. van der Donk, Ry Young, Trevor D. Lawley, Peter Stärkel, David Pride, Derrick E. Fouts, Bernd Schnabl Creator: AH XSL Formatter V6.3 R1 for Linux64 : 6.3.1.23495 (2016/02/03 14:39JST) Producer: Antenna House PDF Output Library 6.3.752 (Linux64) CreationDate: Fri Nov 22 10:32:27 2019 CET ModDate: Fri Nov 22 10:32:27 2019 CET Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 46 Encrypted: no Page size: 612 x 792 pts (letter) Page rot: 0 File size: 5882018 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- Times-Roman Type 1 Custom no no yes 11 0 Helvetica-Bold Type 1 Custom no no yes 13 0 Helvetica Type 1 Custom no no yes 16 0 Helvetica-BoldOblique Type 1 Standard no no no 490 0 XQOWCX+Code2000 CID TrueType Identity-H yes yes yes 549 0 Times-Bold Type 1 Standard no no no 629 0 Times-BoldItalic Type 1 Standard no no no 659 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-08-10 12:18:52 CEST RepresentationInformation: pmc_31723265.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-07-13 03:39:41 CEST Size: 5882018 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Tagged PDF PDFMetadata: Objects: 0 FreeObjects: 140 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: true NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: SUMMARY Item: Title: Cytolysin presence associates with increased mortality Item: Title: Cytolysin promotes ethanol-induced liver disease Item: Title: Bacteriophage treatment attenuates alcohol-related liver disease in mice Item: Title: Discussion Item: Title: Methods Children: Item: Title: Patient cohorts. Item: Title: Mice. Item: Title: Bacteriophages isolation and amplification. Item: Title: Whole-genome sequencing for bacteriophages. Item: Title: Bacteriophage phylogenetic tree. Item: Title: Electron microscopy. Item: Title: Bacterial DNA extraction and 16S rRNA sequencing. Item: Title: Real-time quantitative PCR. Item: Title: E. faecalis isolation and whole-genome sequencing. Item: Title: E. faecalis culture. Item: Title: Fecal Enterococcus level determination. Item: Title: Cytolysin expression and purification. Item: Title: Primary mouse hepatocytes. Item: Title: Biochemical analysis. Item: Title: Staining procedures. Item: Title: Data availability. Item: Title: Code availability. Item: Title: Statistical analysis. Item: Title: Extended Data Item: Title: Extended Data Figure 1. Item: Title: Extended Data Figure 2. Item: Title: Extended Data Figure 3. Item: Title: Extended Data Figure 4. Item: Title: Extended Data Figure 5. Item: Title: Extended Data Figure 6. Item: Title: Extended Data Figure 7. Item: Title: Extended Data Figure 8. Item: Title: Extended Data Figure 9. Item: Title: References Item: Title: Figure 1. Item: Title: Figure 2. Item: Title: Figure 3. Item: Title: Figure 4. 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