6b2881fe989621291fc3f89155677bc6 pmc_31861817.pdf 8de5617966454644e6f45cdfeef06ee50b544ced pmc_31861817.pdf 2fb84bc0ed08c1f212f2537adcb55f5ac9f2217000bb7abb77c4346d43ae4f27 pmc_31861817.pdf Title: Rapid Quantification of Riboflavin in Milk by Front-Face Fluorescence Spectroscopy: A Preliminary Study Subject: The front-face fluorescence spectroscopy technique was used to establish a rapid prediction model of riboflavin concentration in milk without prior sample preparation. The prediction model developed was then compared with two conventional high performance liquid chromatography (HPLC)-based quantification methods. The method of standard addition allowed detecting a linear correlation between fluorescence intensity and riboflavin concentration in 12% (w/w) reconstituted low-heat milk powder. Validation of the model yielded an R2 of 0.99 with a standard error of prediction of 0.13 mg/L. The results suggest a potential use of front-face fluorescence spectroscopy as a simple method for off- and in-line determinations of riboflavin in milk. Keywords: front-face fluorescence; milk; riboflavin; prediction; rapid quantification Author: Ulises Alvarado, Anna Zamora, Jinfang Liu, Jordi Saldo and Manuel Castillo Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri Dec 20 09:53:04 2019 CET ModDate: Fri Dec 20 09:53:04 2019 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 6 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 587192 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YAFSJH+URWPalladioL-Ital Type 1 Custom yes yes no 73 0 WQVYQO+URWPalladioL-Bold Type 1 Custom yes yes no 74 0 SOIQDK+URWPalladioL-Roma Type 1 Custom yes yes no 75 0 OHKNFJ+VnURWPalladioL Type 1 Custom yes yes no 76 0 GGBCKI+Rpxr Type 1 Builtin yes yes no 77 0 XBNVAE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 82 0 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