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Title:          Bone morphogenetic protein 4 gene therapy in mice inhibits myeloma tumor growth, but has a negative impact on bone
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      Title: Bone Morphogenetic Protein 4 Gene Therapy in Mice Inhibits Myeloma Tumor Growth, But Has a Negative Impact on Bone
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       Item: 
        Title: 1  Introduction
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       Item: 
        Title: 2  Materials and Methods
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         Item: 
          Title: 2.1  Cell culture and reagents
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         Item: 
          Title: 2.2  Generation of iRFP-labeled KJON myeloma cells
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         Item: 
          Title: 2.3  Cell viability in vitro
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          Title: 2.4  RAG2-/- γC-/-
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          Title: 2.5  Human-mouse scaffold myeloma model and imaging
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          Title: 2.6  Western blotting
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         Item: 
          Title: 2.7  Real-time RT-PCR
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          Title: 2.8  Osteoclast differentiation
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          Title: 2.9  Scaffold bone analysis
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          Title: 2.10  μCt analysis
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          Title: 2.11  Statistical analysis
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        Title: 3  Results
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         Item: 
          Title: 3.1  BMP4 gene therapy reduces tumor growth in vivo
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          Title: 3.2  The presence of myeloma cells reduces the amount of bone in scaffolds
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         Item: 
          Title: 3.3  High circulating levels of BMP4 reduces mouse trabecular bone volume
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         Item: 
          Title: 3.4  Effects of BMP4 gene therapy on osteoblasts and osteoclasts
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        Title: 4  Discussion
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       Item: 
        Title:   Disclosures
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        Title: Acknowledgments
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       Item: 
        Title:   Authorship Contribution
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       Item: 
        Title: References
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