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Destination: 1 Item: Title: The R. solanacearum PilI and ChpA proteins are involved in twitching but not in swimming motility or chemotaxis. Destination: 1 Item: Title: The R. solanacearum pilI and chpA mutants present reduced natural transformation abilities. Destination: 1 Item: Title: TFP- and flagellin-associated genes are involved in biofilm formation and attachment of R. solanacearum to plant roots. Destination: 1 Item: Title: The pilI and chpA mutants show reduced virulence in soil-soak inoculations but not when directly inoculated in the stem of tomato plants. Destination: 1 Item: Title: Deletion of pilA but not pilI, chpA, and fliC limits bacterial spread in plant tissues. Destination: 1 Item: Title: DISCUSSION Destination: 1 Children: Item: Title: A conserved cluster involved in twitching motility. Destination: 1 Item: Title: TFP influence swimming motility. Destination: 1 Item: Title: R. solanacearum PilI and ChpA play a role in all known TFP-related functions. Destination: 1 Item: Title: Novel roles of flagella in R. solanacearum GMI1000. Destination: 1 Item: Title: Conclusion. Destination: 1 Item: Title: MATERIALS AND METHODS Destination: 1 Children: Item: Title: Bacterial strains, plasmids, plant material, and growth conditions. Destination: 1 Item: Title: RNA extraction and RT-PCR. Destination: 1 Item: Title: Gene identification and construction of R. solanacearum knockouts and of complemented and reporter strains. Destination: 1 Item: Title: Twitching and swimming assays. Destination: 1 Item: Title: Chemotaxis capillary assays. Destination: 1 Item: Title: Natural transformation assays. Destination: 1 Item: Title: Biofilm quantification. Destination: 1 Item: Title: Root attachment quantification. Destination: 1 Item: Title: Bacterial leaf multiplication assays. Destination: 1 Item: Title: Bacterial virulence experiments. Destination: 1 Item: Title: Luminescence assays. Destination: 1 Item: Title: Statistics. Destination: 1 Item: Title: Data accessibility. 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