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Title:          An Alternative Perfusion Approach for the Intensification of Virus-Like Particle Production in HEK293 Cultures
Subject:        Virus-like particles (VLPs) have gained interest over the last years as recombinant vaccine formats, as they generate a strong immune response and present storage and distribution advantages compared to conventional vaccines.
Keywords:       bioreactor, perfusion, ATF, design of experiments, VLP, HFM
Author:         Jesús Lavado-Garcı́a
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      Title: An Alternative Perfusion Approach for the Intensification of Virus-Like Particle Production in HEK293 Cultures
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       Item: 
        Title: Introduction
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       Item: 
        Title: Experimental Section
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         Item: 
          Title: HEK293 Mammalian Cell Line, Culture Conditions
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         Item: 
          Title: Bioreactor Culture Conditions and Setup Description
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         Item: 
          Title: Transient Transfection
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         Item: 
          Title: Flow Cytometry
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         Item: 
          Title: HIV-1 Gag VLP Quantification by Fluorimetry
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         Item: 
          Title: HIV-1 Gag VLP Quantification by Nanoparticle Tracking Analysis
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         Item: 
          Title: Field Emission Scanning Electron Microscopy (FESEM) Visualization
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         Item: 
          Title: Confocal Microscopy Visualization
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         Item: 
          Title: Optimization of Retransfection Parameters Using Design of Experiments
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       Item: 
        Title: Results and Discussion
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         Item: 
          Title: Study of the Cell Growth Upon Different Media Replacement Rates
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         Item: 
          Title: Optimization of Retransfection by Design of Experiment (DoE) Method
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         Item: 
          Title: Optimization of the Second Retransfection Parameters Shake Flasks Study
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         Item: 
          Title: Intensification of the Optimized Protocol in Bioreactor Using ATF
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         Item: 
          Title: Optimization of the Second Retransfection Parameters Bioreactor Study
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         Item: 
          Title: VLP Retention by the ATF Hollow Fiber Module
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         Item: 
          Title: Quantification of VLP Retention
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         Item: 
          Title: Selection of Conditions for a VLP Production Platform
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       Item: 
        Title: Conclusions
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       Item: 
        Title: Data Availability Statement
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       Item: 
        Title: Author Contributions
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       Item: 
        Title: Funding
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       Item: 
        Title: Acknowledgments
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       Item: 
        Title: References
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    Author: Jesús Lavado-Garcı́a
    Subject: Virus-like particles (VLPs) have gained interest over the last years as recombinant vaccine formats, as they generate a strong immune response and present storage and distribution advantages compared to conventional vaccines.
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   Type: CRC32
  Checksum: 123c74df56e29829a4bda840030dd2d9
   Type: MD5
  Checksum: 3a34608ab301c9568c663a821662e1a9392c0854
   Type: SHA-1