213963c76dcd0d190a10302881be4e7a pathogens_a2020v9n7p533.pdf 587bd8cdc0507e1b410c6360c7d18357db401e7d pathogens_a2020v9n7p533.pdf 7c3e3464808387b964151fe29cb8e05fd22c1d45805081a32aa2c4032708fd2a pathogens_a2020v9n7p533.pdf Title: Frequency of Detection and Phylogenetic Analysis of Porcine circovirus 3 (PCV-3) in Healthy Primiparous and Multiparous Sows and Their Mummified Fetuses and Stillborn Subject: Porcine circovirus 3 (PCV-3) has been suggested as a putative causal agent of swine reproductive disease. A number of different studies have pointed out this association, but there is still a lack of information regarding the normal rates of PCV-3 infection in farms with normal reproductive parameters. The objective of the present study was to assess the frequency of PCV-3 detection in primiparous and multiparous sows and in tissues from their respective fetuses from farms with average reproductive parameters. Sera from 57 primiparous and 64 multiparous sows from 3 different farms were collected at two time points. Brain and lung tissues from 49 mummies and 206 stillborn were collected at farrowing. Samples were tested by PCR, and when positive, quantified by quantitative PCR. Thirty-nine complete genomes were obtained and phylogenetically analyzed. All sera from multiparous sows were negative, while 19/57 (33.3%) primiparous sows were PCV-3 PCR positive. From the 255 tested fetuses, 86 (33.7%) had at least one tissue positive to PCV-3. The frequency of detection in fetuses from primiparous sows (73/91, 80.2%) was significantly higher than those from multiparous ones (13/164, 7.9%). It can be concluded that PCV-3 is able to cause intrauterine infections in absence of overt reproductive disorders. Keywords: mummified fetus; stillborn; sow; Porcine circovirus 3; vertical transmission; intra-uterine infection Author: Viviane Saporiti, Susanna Martorell, Taís F. Cruz, Francini Klaumann, Florencia Correa-Fiz, Mònica Balasch, Marina Sibila and Joaquim Segalés Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.17 CreationDate: Thu Jul 2 12:58:50 2020 CEST ModDate: Thu Jul 2 12:58:50 2020 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 797602 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BFVCMW+URWPalladioL-Ital Type 1 Custom yes yes no 80 0 WCVFVS+URWPalladioL-Bold Type 1 Custom yes yes no 81 0 AHHNSI+URWPalladioL-BoldItal Type 1 Custom yes yes no 82 0 BFQLJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 83 0 QTLELQ+Pxbsy Type 1 Builtin yes yes no 84 0 HJXXOE+URWPalladioL-Roma Type 1 Custom yes yes no 85 0 HEOTNM+VnURWPalladioL Type 1 Custom yes yes no 86 0 GJQQCT+Rpxr Type 1 Builtin yes yes no 87 0 YXTFSB+Pxsy Type 1 Builtin yes yes no 88 0 UXHOAG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 94 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 111 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 112 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 113 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 149 0 MFIGJL+Rpxb Type 1 Builtin yes yes no 177 0 XIRVNA+EURB10 Type 1 Builtin yes yes no 178 0 KJCNAO+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 196 0 KJCNIM+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 197 0 KJCNJN+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 198 0 KJCNJP+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 199 0 KJCNKP+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 200 0 KJCOHN+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 201 0 YICMPF+Rpcxr Type 1 Builtin yes yes no 298 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2020-12-19 06:14:24 CET RepresentationInformation: pathogens_a2020v9n7p533.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2020-12-18 11:56:03 CET Size: 797602 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 2 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐぃざ〭〳ぜ〴ぜ〰いづぴづっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ざどひふびぜ〴ぜ〰けふちのぴどてどっちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぐぃざ〭〳ぜ〴ぜ〰ぐとべぬはでづのづぴどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぐぃざ〭〳ぜ〴ぜ〰いづぴづっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ざどひふびぜ〴ぜ〰けふちのぴどてどっちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ぐぃざ〭〳ぜ〴ぜ〰ぐとべぬはでづのづぴどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぐぃざ〭〳ぜ〴ぜ〰ぐとべぬはでづのづぴどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮ㄩ‾㸊⡜㌷㙜㌷㝜〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ぐぃげぜ〴ぜ〰ちのつぜ〴ぜ〰ぱぐぃげぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㌩‾㸊⡜㌷㙜㌷㝜〰ぐぃざ〭〳ぜ〴ぜ〰ぐとべぬはでづのづぴどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Children: Item: Title: こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㈩‾㸊⡜㌷㙜㌷㝜〰いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ぐぃげぜ〴ぜ〰ちのつぜ〴ぜ〰ぱぐぃげぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㌩‾㸊⡜㌷㙜㌷㝜〰ぐぃざ〭〳ぜ〴ぜ〰ぐとべぬはでづのづぴどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: いぎぁぜ〴ぜ〰ぅへぴひちっぴどはの〬ぜ〴ぜ〰ぐぃげぜ〴ぜ〰ちのつぜ〴ぜ〰ぱぐぃげぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㌩‾㸊⡜㌷㙜㌷㝜〰ぐぃざ〭〳ぜ〴ぜ〰ぐとべぬはでづのづぴどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: ぐぃざ〭〳ぜ〴ぜ〰ぐとべぬはでづのづぴどっぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: こぴちぴどびぴどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㐮㐩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: YICMPF+Rpcxr FontSubset: true FirstChar: 151 LastChar: 174 FontDescriptor: FontName: YICMPF+Rpcxr Flags: Symbolic FontBBox: -212, -286, 1344, 973 FontFile: true Font: BaseFont: BFVCMW+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 121 FontDescriptor: FontName: BFVCMW+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WCVFVS+URWPalladioL-Bold FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: WCVFVS+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MFIGJL+Rpxb FontSubset: true FirstChar: 47 LastChar: 47 FontDescriptor: FontName: MFIGJL+Rpxb Flags: Symbolic FontBBox: -57, -266, 932, 729 FontFile: true Font: BaseFont: AHHNSI+URWPalladioL-BoldItal FontSubset: true FirstChar: 51 LastChar: 118 FontDescriptor: FontName: AHHNSI+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XIRVNA+EURB10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: XIRVNA+EURB10 Flags: Symbolic FontBBox: -30, -247, 1178, 723 FontFile: true Font: BaseFont: BFQLJI+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 225 FontDescriptor: FontName: BFQLJI+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: QTLELQ+Pxbsy FontSubset: true FirstChar: 121 LastChar: 121 FontDescriptor: FontName: QTLELQ+Pxbsy Flags: Symbolic FontBBox: 5, -978, 1382, 913 FontFile: true Font: BaseFont: HJXXOE+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 246 FontDescriptor: FontName: HJXXOE+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: OVNEXW+EURM10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: OVNEXW+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: HEOTNM+VnURWPalladioL FontSubset: true FirstChar: 161 LastChar: 243 FontDescriptor: FontName: HEOTNM+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GJQQCT+Rpxr FontSubset: true FirstChar: 11 LastChar: 61 FontDescriptor: FontName: GJQQCT+Rpxr Flags: Symbolic FontBBox: -40, -283, 904, 728 FontFile: true Font: BaseFont: YXTFSB+Pxsy FontSubset: true FirstChar: 2 LastChar: 121 FontDescriptor: FontName: YXTFSB+Pxsy Flags: Symbolic FontBBox: -21, -994, 1292, 912 FontFile: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 75, 757, 208, 793 Annotation: Subtype: Link Rect: 474, 757, 519, 793 Annotation: Subtype: Link Rect: 336, 621, 347, 634 Annotation: Subtype: Link Rect: 352, 607, 363, 621 Annotation: Subtype: Link Rect: 492, 607, 503, 621 Annotation: Subtype: Link Rect: 466, 396, 518, 415 Annotation: Subtype: Link Rect: 408, 76, 415, 85 ActionDest: 312 Annotation: Subtype: Link Rect: 167, 38, 262, 50 Annotation: Subtype: Link Rect: 394, 38, 519, 50 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 436, 743, 443, 752 ActionDest: 312 Annotation: Subtype: Link Rect: 428, 715, 435, 725 ActionDest: 312 Annotation: Subtype: Link Rect: 392, 702, 399, 711 ActionDest: 347 Annotation: Subtype: Link Rect: 344, 688, 351, 698 ActionDest: 347 Annotation: Subtype: Link Rect: 143, 675, 150, 684 ActionDest: 347 Annotation: Subtype: Link Rect: 401, 661, 408, 671 ActionDest: 347 Annotation: Subtype: Link Rect: 411, 661, 418, 671 ActionDest: 347 Annotation: Subtype: Link Rect: 259, 634, 266, 644 ActionDest: 347 Annotation: Subtype: Link Rect: 177, 621, 184, 630 ActionDest: 312 Annotation: Subtype: Link Rect: 184, 621, 191, 630 ActionDest: 312 Annotation: Subtype: Link Rect: 192, 621, 199, 630 ActionDest: 347 Annotation: Subtype: Link Rect: 436, 621, 443, 630 ActionDest: 347 Annotation: Subtype: Link Rect: 443, 621, 455, 630 ActionDest: 347 Annotation: Subtype: Link Rect: 176, 594, 183, 603 ActionDest: 347 Annotation: Subtype: Link Rect: 405, 567, 412, 576 ActionDest: 347 Annotation: Subtype: Link Rect: 412, 567, 419, 576 ActionDest: 347 Annotation: Subtype: Link Rect: 420, 567, 432, 576 ActionDest: 347 Annotation: Subtype: Link Rect: 435, 567, 447, 576 ActionDest: 347 Annotation: Subtype: Link Rect: 202, 540, 214, 549 ActionDest: 347 Annotation: Subtype: Link Rect: 309, 526, 321, 535 ActionDest: 347 Annotation: Subtype: Link Rect: 321, 526, 333, 535 ActionDest: 347 Annotation: Subtype: Link Rect: 446, 499, 453, 508 ActionDest: 347 Annotation: Subtype: Link Rect: 454, 499, 461, 508 ActionDest: 347 Annotation: Subtype: Link Rect: 455, 472, 462, 481 ActionDest: 347 Annotation: Subtype: Link Rect: 297, 459, 304, 468 ActionDest: 347 Annotation: Subtype: Link Rect: 305, 459, 312, 468 ActionDest: 347 Annotation: Subtype: Link Rect: 414, 432, 426, 441 ActionDest: 347 Annotation: Subtype: Link Rect: 426, 432, 438, 441 ActionDest: 347 Annotation: Subtype: Link Rect: 172, 418, 184, 427 ActionDest: 347 Annotation: Subtype: Link Rect: 114, 391, 126, 400 ActionDest: 369 Annotation: Subtype: Link Rect: 200, 228, 207, 240 ActionDest: 173 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 456, 621, 462, 633 ActionDest: 173 Annotation: Subtype: Link Rect: 205, 314, 212, 326 ActionDest: 173 Annotation: Subtype: Link Rect: 465, 233, 472, 245 ActionDest: 173 Annotation: Subtype: Link Rect: 349, 125, 356, 137 ActionDest: 186 Annotation: Subtype: Link Rect: 104, 98, 111, 110 ActionDest: 186 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 175, 365, 182, 377 ActionDest: 191 Annotation: Subtype: Link Rect: 323, 354, 335, 363 ActionDest: 369 Annotation: Subtype: Link Rect: 125, 300, 137, 309 ActionDest: 369 Page: Sequence: 5 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 161, 625, 168, 634 ActionDest: 347 Annotation: Subtype: Link Rect: 397, 597, 409, 607 ActionDest: 347 Annotation: Subtype: Link Rect: 261, 571, 273, 580 ActionDest: 347 Annotation: Subtype: Link Rect: 362, 543, 374, 553 ActionDest: 347 Annotation: Subtype: Link Rect: 267, 503, 279, 512 ActionDest: 347 Annotation: Subtype: Link Rect: 417, 489, 429, 499 ActionDest: 369 Annotation: Subtype: Link Rect: 423, 435, 430, 445 ActionDest: 347 Annotation: Subtype: Link Rect: 466, 381, 478, 391 ActionDest: 347 Annotation: Subtype: Link Rect: 478, 381, 490, 391 ActionDest: 347 Annotation: Subtype: Link Rect: 491, 381, 503, 391 ActionDest: 347 Annotation: Subtype: Link Rect: 503, 381, 515, 391 ActionDest: 369 Annotation: Subtype: Link Rect: 89, 325, 462, 336 Annotation: Subtype: Link Rect: 442, 246, 454, 255 ActionDest: 369 Annotation: Subtype: Link Rect: 228, 206, 240, 215 ActionDest: 369 Annotation: Subtype: Link Rect: 430, 192, 442, 201 ActionDest: 369 Annotation: Subtype: Link Rect: 311, 111, 318, 120 ActionDest: 347 Annotation: Subtype: Link Rect: 318, 111, 330, 120 ActionDest: 369 Annotation: Subtype: Link Rect: 330, 111, 342, 120 ActionDest: 369 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 201, 702, 213, 711 ActionDest: 369 Annotation: Subtype: Link Rect: 184, 675, 196, 684 ActionDest: 369 Annotation: Subtype: Link Rect: 457, 634, 469, 644 ActionDest: 347 Annotation: Subtype: Link Rect: 108, 580, 120, 590 ActionDest: 347 Annotation: Subtype: Link Rect: 169, 567, 181, 576 ActionDest: 369 Annotation: Subtype: Link Rect: 503, 540, 515, 549 ActionDest: 369 Annotation: Subtype: Link Rect: 365, 499, 377, 508 ActionDest: 369 Annotation: Subtype: Link Rect: 347, 337, 359, 346 ActionDest: 347 Annotation: Subtype: Link Rect: 360, 337, 372, 346 ActionDest: 369 Annotation: Subtype: Link Rect: 372, 337, 384, 346 ActionDest: 369 Annotation: Subtype: Link Rect: 385, 337, 396, 346 ActionDest: 369 Annotation: Subtype: Link Rect: 397, 337, 409, 346 ActionDest: 369 Annotation: Subtype: Link Rect: 471, 283, 482, 292 ActionDest: 369 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 332, 629, 493, 641 Annotation: Subtype: Link Rect: 105, 508, 112, 520 ActionDest: 173 Annotation: Subtype: Link Rect: 358, 467, 365, 479 ActionDest: 173 Annotation: Subtype: Link Rect: 293, 443, 305, 452 ActionDest: 369 Annotation: Subtype: Link Rect: 318, 429, 330, 439 ActionDest: 369 Annotation: Subtype: Link Rect: 124, 319, 131, 328 ActionDest: 347 Annotation: Subtype: Link Rect: 271, 292, 278, 301 ActionDest: 347 Annotation: Subtype: Link Rect: 278, 292, 290, 301 ActionDest: 369 Annotation: Subtype: Link Rect: 433, 209, 445, 218 ActionDest: 347 Annotation: Subtype: Link Rect: 117, 87, 129, 97 ActionDest: 369 Annotation: Subtype: Link Rect: 504, 87, 516, 97 ActionDest: 369 Annotation: Subtype: Link Rect: 444, 74, 456, 83 ActionDest: 369 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 195, 729, 207, 738 ActionDest: 369 Annotation: Subtype: Link Rect: 163, 715, 175, 725 ActionDest: 369 Annotation: Subtype: Link Rect: 364, 688, 376, 698 ActionDest: 369 Annotation: Subtype: Link Rect: 143, 661, 154, 671 ActionDest: 369 Annotation: Subtype: Link Rect: 187, 634, 199, 644 ActionDest: 369 Annotation: Subtype: Link Rect: 406, 621, 418, 630 ActionDest: 377 Annotation: Subtype: Link Rect: 425, 607, 437, 617 ActionDest: 377 Annotation: Subtype: Link Rect: 446, 419, 519, 430 Annotation: Subtype: Link Rect: 75, 408, 179, 419 Annotation: Subtype: Link Rect: 259, 102, 297, 112 Annotation: Subtype: Link Rect: 208, 76, 245, 86 Annotation: Subtype: Link Rect: 252, 76, 288, 86 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 132, 715, 169, 725 Annotation: Subtype: Link Rect: 176, 715, 212, 725 Annotation: Subtype: Link Rect: 360, 675, 398, 686 Annotation: Subtype: Link Rect: 404, 675, 440, 686 Annotation: Subtype: Link Rect: 184, 637, 221, 648 Annotation: Subtype: Link Rect: 227, 637, 263, 648 Annotation: Subtype: Link Rect: 272, 598, 310, 609 Annotation: Subtype: Link Rect: 316, 598, 352, 609 Annotation: Subtype: Link Rect: 127, 560, 165, 570 Annotation: Subtype: Link Rect: 360, 521, 398, 531 Annotation: Subtype: Link Rect: 154, 483, 192, 493 Annotation: Subtype: Link Rect: 378, 456, 415, 467 Annotation: Subtype: Link Rect: 234, 417, 272, 428 Annotation: Subtype: Link Rect: 278, 417, 314, 428 Annotation: Subtype: Link Rect: 478, 391, 516, 402 Annotation: Subtype: Link Rect: 100, 379, 136, 389 Annotation: Subtype: Link Rect: 243, 340, 280, 351 Annotation: Subtype: Link Rect: 286, 340, 322, 351 Annotation: Subtype: Link Rect: 386, 301, 424, 312 Annotation: Subtype: Link Rect: 397, 262, 434, 273 Annotation: Subtype: Link Rect: 367, 224, 405, 234 Annotation: Subtype: Link Rect: 212, 185, 250, 196 Annotation: Subtype: Link Rect: 209, 147, 246, 157 Annotation: Subtype: Link Rect: 100, 108, 137, 118 Annotation: Subtype: Link Rect: 295, 69, 333, 80 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 349, 727, 387, 738 Annotation: Subtype: Link Rect: 393, 727, 429, 738 Annotation: Subtype: Link Rect: 136, 689, 174, 699 Annotation: Subtype: Link Rect: 180, 689, 216, 699 Annotation: Subtype: Link Rect: 225, 638, 262, 648 Annotation: Subtype: Link Rect: 269, 638, 305, 648 Annotation: Subtype: Link Rect: 299, 599, 337, 609 Annotation: Subtype: Link Rect: 181, 560, 219, 570 Annotation: Subtype: Link Rect: 172, 521, 210, 531 Annotation: Subtype: Link Rect: 455, 443, 493, 454 Annotation: Subtype: Link Rect: 132, 392, 169, 402 Annotation: Subtype: Link Rect: 145, 354, 182, 364 Annotation: Subtype: Link Rect: 437, 249, 474, 260 Annotation: Subtype: Link Rect: 481, 249, 516, 260 Annotation: Subtype: Link Rect: 172, 199, 210, 209 Annotation: Subtype: Link Rect: 216, 199, 252, 209 Annotation: Subtype: Link Rect: 281, 172, 319, 183 Annotation: Subtype: Link Rect: 325, 172, 361, 183 Annotation: Subtype: Link Rect: 359, 146, 397, 157 Annotation: Subtype: Link Rect: 222, 121, 259, 131 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 252, 727, 290, 738 Annotation: Subtype: Link Rect: 76, 659, 166, 690 Annotation: Subtype: Link Rect: 234, 654, 407, 664 Checksum: b73b38a9 Type: CRC32 Checksum: 213963c76dcd0d190a10302881be4e7a Type: MD5 Checksum: 587bd8cdc0507e1b410c6360c7d18357db401e7d Type: SHA-1