3d0fd3499d390e9367e684c661406b38 biology_a2020v9n9p262.pdf 4a672c47a6a50588bb4b9287805f23462342b38c biology_a2020v9n9p262.pdf 92f0cce13b8f996d475423f5e1d9a43f9cd328d56c5f91c17cabeeb2a5f71687 biology_a2020v9n9p262.pdf Title: Relationship of Seminal Oxidation-Reduction Potential with Sperm DNA Integrity and pH in Idiopathic Infertile Patients Subject: Seminal oxidative stress (OS) is one of the most promising factors to describe the causes of idiopathic male infertility. Redox balance is essential in several biological processes related to fertility, so alterations such as high reactive oxygen species (ROS) levels or low antioxidant agent levels can compromise it. MiOXSYS has been developed to evaluate the seminal static oxidation-reduction potential (sORP) and it has been proposed as an effective diagnostic biomarker. However, its relationship with parameters like sperm DNA fragmentation (SDF), chromatin compaction status or seminal pH requires further analysis, making it the object of this study. Semen and sORP analysis were performed for all samples. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay (TUNEL) and Comet assay were used to assess SDF and chromomycin a3 (CMA3) test to assess sperm chromatin compaction. Regarding sORP measures, it was found that alkaline pH has an effect on sample reproducibility. To our knowledge, this unexpected effect has not been previously described. A statistical analysis showed that sORP correlated negatively with CMA3 positive cells and sperm motility, but not with SDF. As redox dysregulation, which occurs mainly at the testicular and epididymal level, causes chromatin compaction problems and leaves DNA exposed to damage, an excess of ROS could be counterbalanced further by a seminal supply of antioxidant molecules, explaining the negative correlation with CMA3 positive cells but no correlation with SDF. Our results show that the study of idiopathic infertility would benefit from a combined approach comprising OS analysis, SDF and chromatin compaction analysis. Keywords: sperm; oxidative stress; oxidation-reduction potential; sperm DNA fragmentation; DNA damage; comet assay; DNA compaction; chromomycin A3; pH; male infertility Author: Sergio Garcia-Segura, Jordi Ribas-Maynou, Sandra Lara-Cerrillo, Agustín Garcia-Peiró, Ana Belén Castel, Jordi Benet and Maria Oliver-Bonet Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Tue Sep 1 12:23:47 2020 CEST ModDate: Tue Sep 1 12:23:47 2020 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 522421 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BGYZTK+URWPalladioL-Ital Type 1 Custom yes yes no 85 0 KBGJQP+URWPalladioL-Bold Type 1 Custom yes yes no 86 0 XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes no 87 0 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