2c9db4e3ddb38d0af4cda3ee431907c1 cells_a2020v9n10p2235.pdf c19b8e00df980838693a4549a421e78de03a83e9 cells_a2020v9n10p2235.pdf 77277e0151c9c69131787545e02e4e0e0e54b837da69149d7cbdd592b889f40d cells_a2020v9n10p2235.pdf Title: Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells Subject: Background: The increase in the incidence of obesity and obesity-related cardiovascular risk factors (CVRFs) over the last decades has brought attention on adipose tissue (AT) pathobiology. The expansion of AT is associated with the development of new vasculature needed to perfuse the tissue; however, not all fat depots have the same ability to induce angiogenesis that requires recruitment of their own endothelial cells. In this study we have investigated the effect of different CVRFs, on the angiogenic capacity of the subcutaneous (SAT) and visceral (VAT) adipose tissue and on the function of their mesenchymal cell reservoir. Methods: A transcriptomic approach was used to compare the different angiogenic and inflammatory profiles of the subcutaneous and visceral fat depots from individuals with obesity, as well as their resident stem cells (ASCs). Influence of other risk factors on fat composition was also measured. Finally, the microvesicles (MVs) released by ASCs were isolated and their regenerative potential analyzed by molecular and cellular methodologies. Results: Obesity decreases the angiogenic capacity of AT. There are differences between SAT and VAT; from the 21 angiogenic-related genes analyzed, only three were decreased in SAT compared with those decreased in VAT. ASCs isolated from both fat depots showed significant differences; there was a significant up-regulation of the VEGF-pathway on visceral derived ASCs. ASCs release MVs that stimulate endothelial cell migration and angiogenic capacity. Conclusions: In patients with obesity, SAT expresses a greater number of angiogenic molecules than VAT, independent of the presence of other CVRFs. Keywords: adipose stem cells; endothelial cells; microvesicles; obesity; cardiovascular risk factors; and angiogenesis Author: Gemma Arderiu, Carmen Lambert, Carlos Ballesta, Fabrizio Moscatiello, Gemma Vilahur and Lina Badimon Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Sat Oct 10 14:31:06 2020 CEST ModDate: Mon Oct 12 00:48:58 2020 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5393557 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LLAMBZ+Pxsy Type 1 Builtin yes yes no 10 0 AZBFJT+VnURWPalladioL Type 1 Custom yes yes no 14 0 NSJOBV+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 FHKZMZ+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 RQODNN+Rpxb Type 1 Builtin yes yes no 28 0 IQJJXZ+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 SYXPPI+Rpxr Type 1 Builtin yes yes no 36 0 QTLELQ+Pxbsy Type 1 Builtin yes yes no 40 0 PFKOHU+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 70 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 74 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 78 0 LEVXPH+EURM10 Type 1 Builtin yes yes no 88 0 JNLIIF+Rpxi Type 1 Builtin yes yes no 95 0 YICMPF+Rpcxr Type 1 Builtin yes yes no 99 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 103 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 119 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:25:05 CEST RepresentationInformation: cells_a2020v9n10p2235.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:39:31 CEST Size: 5393557 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 418 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Patient Recruitment and Adipose Tissue Sampling Destination: subsection.2.1 Item: Title: RNA and cDNA Isolation from Adipose Tissue Destination: subsection.2.2 Item: Title: Transcriptomic Analysis and RT-PCR Destination: subsection.2.3 Item: Title: Bioinformatic Analysis Destination: subsection.2.4 Item: Title: ASCs Isolation and Characterization Destination: subsection.2.5 Item: Title: MTS Viability/Proliferation Analysis Destination: subsection.2.6 Item: Title: Microvesicles Isolation Destination: subsection.2.7 Item: Title: Cell Migration Destination: subsection.2.8 Item: Title: Gene Expression Analysis from ASCs Destination: subsection.2.9 Item: Title: Western Blot Analysis Destination: subsection.2.10 Item: Title: Statistical Analysis Destination: subsection.2.11 Item: Title: Results Destination: section.3 Children: Item: Title: Influence of Obesity on the Subcutaneous Adipose Tissue Destination: subsection.3.1 Item: Title: Influence of Obesity in Fat Depot Genomic Profile Destination: subsection.3.2 Item: Title: Angiogenic Related Gene Expression in Subcutaneous or Visceral Derived ASCs from Diabetic/No Diabetic Patients with Obesity Destination: subsection.3.3 Item: Title: Subcutaneous and Visceral ASCs Derived Microvesicles and Effects on Wound Repair Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: References Destination: figure.caption.12 Info: Title: Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells Author: Gemma Arderiu, Carmen Lambert, Carlos Ballesta, Fabrizio Moscatiello, Gemma Vilahur and Lina Badimon Subject: Background: The increase in the incidence of obesity and obesity-related cardiovascular risk factors (CVRFs) over the last decades has brought attention on adipose tissue (AT) pathobiology. The expansion of AT is associated with the development of new vasculature needed to perfuse the tissue; however, not all fat depots have the same ability to induce angiogenesis that requires recruitment of their own endothelial cells. In this study we have investigated the effect of different CVRFs, on the angiogenic capacity of the subcutaneous (SAT) and visceral (VAT) adipose tissue and on the function of their mesenchymal cell reservoir. Methods: A transcriptomic approach was used to compare the different angiogenic and inflammatory profiles of the subcutaneous and visceral fat depots from individuals with obesity, as well as their resident stem cells (ASCs). Influence of other risk factors on fat composition was also measured. Finally, the microvesicles (MVs) released by ASCs were isolated and their regenerative potential analyzed by molecular and cellular methodologies. Results: Obesity decreases the angiogenic capacity of AT. There are differences between SAT and VAT; from the 21 angiogenic-related genes analyzed, only three were decreased in SAT compared with those decreased in VAT. ASCs isolated from both fat depots showed significant differences; there was a significant up-regulation of the VEGF-pathway on visceral derived ASCs. ASCs release MVs that stimulate endothelial cell migration and angiogenic capacity. Conclusions: In patients with obesity, SAT expresses a greater number of angiogenic molecules than VAT, independent of the presence of other CVRFs. 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