49cb083ab182cf966aaca1887fdeba63 cancers_a2020v12n9p2644.pdf 42d53d15caf36bdd24b11de4cf3f5858344da700 cancers_a2020v12n9p2644.pdf 1f16939845a77d22050e7147b81ce613809ed82febcbc1d2ac22cbe855a3e0d7 cancers_a2020v12n9p2644.pdf Title: B Cells and Tertiary Lymphoid Structures Influence Survival in Lung Cancer Patients with Resectable Tumors Subject: Immune profile of B and T cells and tertiary lymphoid structures (TLSs) may differ in tumors of lung cancer (LC) patients with/without chronic obstructive pulmonary disease (COPD), and may also influence patient survival. We sought to analyze: (1) TLSs, germinal centers (GCs), B and T cells, and (2) associations of the immune biomarkers with the patients’ 10-year overall survival (OS). TLSs (numbers and area), B [cluster of differentiation (CD) 20], and T (CD3), and GCs cells were identified in both tumor and non-tumor specimens (thoracotomy) from 90 LC-COPD patients and 43 LC-only patients. Ten-year OS was analyzed in the patients. Immune profile in tumors of LC-COPD versus LC: TLS numbers and areas significantly decreased in tumors of LC-COPD compared to LC patients. No significant differences were observed in tumors between LC-COPD and LC patients for B or T cells. Immune profile in tumors versus non-tumor specimens: TLS areas and B cells significantly increased, T cells significantly decreased in tumors of both LC and LC-COPD patients. Survival: in LC-COPD patients: greater area of TLSs and proportion of B cells were associated with longer survival rates. The immune tumor microenvironment differs in patients with underlying COPD and these different phenotypes may eventually impact the response to immunotherapy in patients with LC. Keywords: lung cancer; chronic respiratory diseases; tertiary lymphoid structures; B cells; overall survival Author: Jun Tang, Daniel Ramis-Cabrer, Víctor Curull, Xuejie Wang, Mercé Mateu-Jiménez, Lara Pijuan, Xavier Duran, Liyun Qin, Alberto Rodríguez-Fuster, Rafael Aguiló and Esther Barreiro Creator: LaTeX with hyperref Producer: MiKTeX pdfTeX-1.40.21 CreationDate: Wed Sep 16 12:39:15 2020 CEST ModDate: Wed Sep 16 12:39:15 2020 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2820246 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JJLEGW+URWPalladioL-Ital Type 1 Custom yes yes no 104 0 AOEYJF+URWPalladioL-Bold Type 1 Custom yes yes no 105 0 XWOCXD+VnURWPalladioL-Bold Type 1 Custom yes yes no 106 0 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FNOBFJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 247 0 FNOBFK+PalatinoLinotype TrueType WinAnsi yes yes no 248 0 FNOBDG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 279 0 FNOBEI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 280 0 FNOBFK+PalatinoLinotype TrueType WinAnsi yes yes no 281 0 FNOBDG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 301 0 FNOBEI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 302 0 FNOBFJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 303 0 FNOBFK+PalatinoLinotype TrueType WinAnsi yes yes no 304 0 FNODHA+Arial CID TrueType Identity-H yes yes yes 305 0 FNOBDG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 330 0 FNOBEI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 331 0 FNOBFJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 332 0 FNOBFK+PalatinoLinotype TrueType WinAnsi yes yes no 333 0 VSHNAE+Rpcxr Type 1 Builtin yes yes no 541 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 04:24:56 CEST RepresentationInformation: cancers_a2020v12n9p2644.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:39:33 CEST Size: 2820246 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 606 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはの〠 Item: Title: げづびふぬぴび〠 Children: Item: Title: ぃぬどのどっちぬ〠ぃとちひちっぴづひどびぴどっび〠はて〠ぴとづ〠こぴふつべ〠ぐちぴどづのぴび〠 Item: Title: ぎふねぢづひ〠ちのつ〠ぁひづち〠はて〠ごがこび〠ちのつ〠ぎふねぢづひ〠はて〠ぇぃび〠どの〠がふので〠こちねばぬづび〠 Children: Item: Title: いどててづひづのっづび〠ぢづぴぷづづの〠がぃ〭ぃくぐい〠ちのつ〠がぃ〠どの〠ぅどぴとづひ〠ごふねはひ〠はひ〠ぎはの〭ごふねはひ〠がふので〠こちねばぬづび〠 Item: Title: いどててづひづのっづび〠ぢづぴぷづづの〠ごふねはひ〠ちのつ〠ぎはの〭ごふねはひ〠がふので〠こちねばぬづび〠どの〠がぃ〭ぃくぐい〠ちのつ〠がぃ〠ぐちぴどづのぴび〠 Item: Title: ご〠ちのつ〠あ〠ぃづぬぬ〠がづぶづぬび〠どの〠がふので〠こちねばぬづび〠 Children: Item: Title: いどててづひづのっづび〠ぢづぴぷづづの〠がぃ〭ぃくぐい〠ちのつ〠がぃ〠どの〠づどぴとづひ〠ごふねはひ〠はひ〠ぎはの〭ごふねはひ〠がふので〠こちねばぬづび〠 Item: Title: いどててづひづのっづび〠ぢづぴぷづづの〠ごふねはひ〠ちのつ〠ぎはの〭ごふねはひ〠がふので〠こちねばぬづび〠どの〠がぃ〭ぃくぐい〠ちのつ〠がぃ〠ぐちぴどづのぴび〠 Item: Title: ぁびびはっどちぴどはのび〠はて〠ごがこび〠ぷどぴと〠くこ〠どの〠がぃ〠ちのつ〠がぃ〭ぃくぐい〠ぐちぴどづのぴび〠 Item: Title: ぁびびはっどちぴどはのび〠はて〠あ〠ちのつ〠ご〠ぃづぬぬび〠ぷどぴと〠こふひぶどぶちぬ〠どの〠がぃ〠ちのつ〠がぃ〭ぃくぐい〠ぐちぴどづのぴび〠 Item: Title: いどびっふびびどはの〠 Item: Title: きちぴづひどちぬび〠ちのつ〠きづぴとはつび〠 Children: Item: Title: こぴふつべ〠いづびどでの〠ちのつ〠ぅぴとどっび〠 Item: Title: ぃぬどのどっちぬ〠ぁびびづびびねづのぴ〠 Item: Title: こちねばぬづ〠ぃはぬぬづっぴどはの〠ちのつ〠ぐひづびづひぶちぴどはの〠 Item: Title: ぉつづのぴどてどっちぴどはの〠はて〠あ〠ぃづぬぬび〬〠ご〠ぃづぬぬび〬〠ちのつ〠ごがこび〠どの〠ぴとづ〠がふので〠こばづっどねづのび〠 Item: Title: ぉつづのぴどてどっちぴどはの〠はて〠ぇぃび〠どの〠ごがこび〠はて〠がふので〠こばづっどねづのび〠 Item: Title: こぴちぴどびぴどっちぬ〠ぁのちぬべびづび〠 Item: Title: ぃはのっぬふびどはのび〠 Item: Title: げづてづひづのっづび Info: Title: あ〠ぃづぬぬび〠ちのつ〠ごづひぴどちひべ〠がべねばとはどつ〠こぴひふっぴふひづび〠ぉのてぬふづのっづ〠こふひぶどぶちぬ〠どの〠がふので〠ぃちのっづひ〠ぐちぴどづのぴび〠ぷどぴと〠げづびづっぴちぢぬづ〠ごふねはひび Author: おふの〠ごちので〬〠いちのどづぬ〠げちねどび〭ぃちぢひづひ〬〠ざロっぴはひ〠ぃふひふぬぬ〬〠じふづなどづ〠しちので〬〠きづひっラ〠きちぴづふ〭おどねラのづぺ〬〠がちひち〠ぐどなふちの〬〠じちぶどづひ〠いふひちの〬〠がどべふの〠けどの〬〠ぁぬぢづひぴは〠げはつひロでふづぺ〭うふびぴづひ〬〠げちてちづぬ〠ぁでふどぬン〠ちのつ〠ぅびぴとづひ〠あちひひづどひは Subject: ぉねねふのづ〠ばひはてどぬづ〠はて〠あ〠ちのつ〠ご〠っづぬぬび〠ちのつ〠ぴづひぴどちひべ〠ぬべねばとはどつ〠びぴひふっぴふひづび〠〨ごがこび〩〠ねちべ〠つどててづひ〠どの〠ぴふねはひび〠はて〠ぬふので〠っちのっづひ〠〨がぃ〩〠ばちぴどづのぴび〠ぷどぴと〯ぷどぴとはふぴ〠っとひはのどっ〠はぢびぴひふっぴどぶづ〠ばふぬねはのちひべ〠つどびづちびづ〠〨ぃくぐい〩〬〠ちのつ〠ねちべ〠ちぬびは〠どのてぬふづのっづ〠ばちぴどづのぴ〠びふひぶどぶちぬ〮〠しづ〠びはふでとぴ〠ぴは〠ちのちぬべぺづ〺〠〨〱〩〠ごがこび〬〠でづひねどのちぬ〠っづのぴづひび〠〨ぇぃび〩〬〠あ〠ちのつ〠ご〠っづぬぬび〬〠ちのつ〠〨〲〩〠ちびびはっどちぴどはのび〠はて〠ぴとづ〠どねねふのづ〠ぢどはねちひにづひび〠ぷどぴと〠ぴとづ〠ばちぴどづのぴび〙〠〱〰〭べづちひ〠はぶづひちぬぬ〠びふひぶどぶちぬ〠〨くこ〩〮〠ごがこび〠〨のふねぢづひび〠ちのつ〠ちひづち〩〬〠あ〠せっぬふびぴづひ〠はて〠つどててづひづのぴどちぴどはの〠〨ぃい〩〠〲〰そ〬〠ちのつ〠ご〠〨ぃい〳〩〬〠ちのつ〠ぇぃび〠っづぬぬび〠ぷづひづ〠どつづのぴどてどづつ〠どの〠ぢはぴと〠ぴふねはひ〠ちのつ〠のはの〭ぴふねはひ〠びばづっどねづのび〠〨ぴとはひちっはぴはねべ〩〠てひはね〠〹〰〠がぃ〭ぃくぐい〠ばちぴどづのぴび〠ちのつ〠〴〳〠がぃ〭はのぬべ〠ばちぴどづのぴび〮〠ごづの〭べづちひ〠くこ〠ぷちび〠ちのちぬべぺづつ〠どの〠ぴとづ〠ばちぴどづのぴび〮〠ぉねねふのづ〠ばひはてどぬづ〠どの〠ぴふねはひび〠はて〠がぃ〭ぃくぐい〠ぶづひびふび〠がぃ〺〠ごがこ〠のふねぢづひび〠ちのつ〠ちひづちび〠びどでのどてどっちのぴぬべ〠つづっひづちびづつ〠どの〠ぴふねはひび〠はて〠がぃ〭ぃくぐい〠っはねばちひづつ〠ぴは〠がぃ〠ばちぴどづのぴび〮〠ぎは〠びどでのどてどっちのぴ〠つどててづひづのっづび〠ぷづひづ〠はぢびづひぶづつ〠どの〠ぴふねはひび〠ぢづぴぷづづの〠がぃ〭ぃくぐい〠ちのつ〠がぃ〠ばちぴどづのぴび〠てはひ〠あ〠はひ〠ご〠っづぬぬび〮〠ぉねねふのづ〠ばひはてどぬづ〠どの〠ぴふねはひび〠ぶづひびふび〠のはの〭ぴふねはひ〠びばづっどねづのび〺〠ごがこ〠ちひづちび〠ちのつ〠あ〠っづぬぬび〠びどでのどてどっちのぴぬべ〠どのっひづちびづつ〬〠ご〠っづぬぬび〠びどでのどてどっちのぴぬべ〠つづっひづちびづつ〠どの〠ぴふねはひび〠はて〠ぢはぴと〠がぃ〠ちのつ〠がぃ〭ぃくぐい〠ばちぴどづのぴび〮〠こふひぶどぶちぬ〺〠どの〠がぃ〭ぃくぐい〠ばちぴどづのぴび〺〠でひづちぴづひ〠ちひづち〠はて〠ごがこび〠ちのつ〠ばひはばはひぴどはの〠はて〠あ〠っづぬぬび〠ぷづひづ〠ちびびはっどちぴづつ〠ぷどぴと〠ぬはのでづひ〠びふひぶどぶちぬ〠ひちぴづび〮〠ごとづ〠どねねふのづ〠ぴふねはひ〠ねどっひはづのぶどひはのねづのぴ〠つどててづひび〠どの〠ばちぴどづのぴび〠ぷどぴと〠ふのつづひぬべどので〠ぃくぐい〠ちのつ〠ぴとづびづ〠つどててづひづのぴ〠ばとづのはぴべばづび〠ねちべ〠づぶづのぴふちぬぬべ〠どねばちっぴ〠ぴとづ〠ひづびばはのびづ〠ぴは〠どねねふのはぴとづひちばべ〠どの〠ばちぴどづのぴび〠ぷどぴと〠がぃ〮 Keywords: ぬふので〠っちのっづひ〻〠っとひはのどっ〠ひづびばどひちぴはひべ〠つどびづちびづび〻〠ぴづひぴどちひべ〠ぬべねばとはどつ〠びぴひふっぴふひづび〻〠あ〠っづぬぬび〻〠はぶづひちぬぬ〠びふひぶどぶちぬ Creator: LaTeX with hyperref Producer: MiKTeX pdfTeX-1.40.21 CreationDate: Wed Sep 16 12:39:15 CEST 2020 ModDate: Wed Sep 16 12:39:15 CEST 2020 ID: 0x6e0e2ee30b7f4dd921ec80b7fe130384, 0x6e0e2ee30b7f4dd921ec80b7fe130384 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: URYLRZ+URWPalladioL-BoldItal FontSubset: true FirstChar: 110 LastChar: 110 FontDescriptor: FontName: URYLRZ+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: VUPETJ+Rpxb FontSubset: true FirstChar: 61 LastChar: 61 FontDescriptor: FontName: VUPETJ+Rpxb Flags: Symbolic FontBBox: -57, -266, 932, 729 FontFile: true Font: BaseFont: JJLEGW+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 121 FontDescriptor: FontName: JJLEGW+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true 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