2000


A 1600                                             1800

                                                   1600
                                                                                                                      BB
                                                                                                                       1200                                              1200

                                                                                                                                                                                     -AMD3100
                                                                                                                                                                                     - AMD
                                                                                                                                                                                     + AMD
                                                                                                                                                                                                                            C           Control 0 h
                                                                                                                                                                         1000        +AMD3100
                                                   1400
                          Internalization (a.u.)
Internalization (units)




                                                                                                                      Internalization (units)
                                                                                                                                                Internalization (a.u.)
                          1200
                            1200                                                                                                                                800
                                                                                                                                                                  800

                                                   1000
                                                                                                                                                                         600
                                     800
                                       800

                                                   600
                                                                                                                                                                400
                                                                                                                                                                  400


                                     400
                                       400

                                                   200
                                                                                                                                                                         200
                                                                                                                                                                                                                                      Bar size: 20 µm
                                                    00                                                                                                                    00
                                                          T22-BAK-GFP-H6    H6                H6                 H6
                                                                                            P- T22-BAXPORO-GFP-H6                                                               00    100        500         1000    2000
                                                                    - GF
                                                                         P-
                                                                                       - GF              - GF
                                                                                                              P-                                                                     100        500         1000     2000
                                                                  AK                  A                O                                                                                        Concentration (nM)
                                                           T2
                                                              2-B              T22-PUMA-GFP-H6
                                                                                2-P
                                                                                    UM
                                                                                                 XP
                                                                                                    OR                                                                                       Concentration      (nM)
                                                                        T2               A
                                                                                      2-B
                                                                                    T2

                                                                  30 min                                                                                                 1h                                          3h                           24 h




                                                                                                                                                                                                                            Size bar: 20 µm