A    # comparing to control                                                         B                                        30
                                    30
                                         & comparing to at least one nanoparticle alone                                                                                                                                       & &                 &
                                                                                                                                                                 25
                                                                                                                                                                                              *                   *




                                                                                                                                    Apoptotic cells per field
                                    25                                                                                                                                                                                            # #     # #
         Mitotic cells per field




                                            5h           24h                                            *           *
                                                                                                                                                                 20                                                   #
                                    20                                                                                                                                        # #       # #       #
                                                     *                                                                                                           15
                                    15                                              &                       &                                                                                                 #
                                    10                                                                                                                           10
                                                               #       # #
                                                 #                                                  #
                                    5                                                                                                                             5

                                    0                                                                                                                             0




                                                                                        *
                                    C                                   *
                                                                                            &   &               &       D
                                                                                                                                                                100                                                           *
                                    30                                                      #   # #             # #
                                                                            #                                                                                                                                 *
                                    25                   #         #                &                                                                           80                                                                &
             Dead cells per field




                                                                                                                              Tumor necrosis %
                                                                                    #                                                                                                         #                   #               #
                                    20                                                                                                                          60
                                                                                                                                                                                                                                              #
                                    15
                                                                                                                                                                40
                                    10
                                                                                                                                                                20
                                     5
                                                                                                                                                                 0
                                     0




                                    E                                                                                   F
                                    25                                                                                                                          25
                                                                                                                                                                          *         *
Staining active-caspase 3




                                                                                                                        Staining proteolyzed PARP




                                    20                                                                                                                          20    *        *
                                                                                                                                                                                                                          *
      cells per field




                                                                                                                               cells per field




                                    15                                                                                                                          15                        *
                                                                                                                                                                                                      *                   *

                                    10
                                                                                *               *               *                                               10


                                     5                                                                                                                           5

                                                                                    &               &                                                                                                     &                   &         & &
                                     0                                                                                                                           0